Thu, Feb 20, 2025
Volume 11, Issue 5 (Sep - Oct 2017)
mljgoums 2017, 11(5): 36-40 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Abouhosseini Tabari M, Youssefi M R, Hosseini Farash B R, Ebrahimi M A, Nabavi Mousavi N, Moghaddas E. Anti-Leishmanial Activity of Pelargonium roseum Essential Oil on Growth of Leishmania infantum Promastigotes . mljgoums 2017; 11 (5) :36-40
URL: http://mlj.goums.ac.ir/article-1-1009-en.html
URL: http://mlj.goums.ac.ir/article-1-1009-en.html
Mohaddeseh Abouhosseini Tabari1 
, Mohammad Reza Youssefi2 , Bibi Razieh Hosseini Farash3 , Mohammad Amin Ebrahimi4 , Niki Nabavi Mousavi5 , Elham Moghaddas 6
, Mohammad Reza Youssefi2 , Bibi Razieh Hosseini Farash3 , Mohammad Amin Ebrahimi4 , Niki Nabavi Mousavi5 , Elham Moghaddas 6
1- Faculty of Veterinary Medicine, Amol University of Special ModernTechnologies, Amol, Iran
2- Department of Veterinary Parasitology, Babol-Branch, Islamic Azad University, Babol, Iran
3- Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4- Young Researcher and Elite Club, Babol -Branch,Islamic Azad University, Babol-Branch, Babol, Iran
5- Young Researcher and Elite Club, Babol- Branch, Islamic Azad University, Babol-Branch, Babol, Iran
6- Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran ,moghaddase@mums.ac.ir
2- Department of Veterinary Parasitology, Babol-Branch, Islamic Azad University, Babol, Iran
3- Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4- Young Researcher and Elite Club, Babol -Branch,Islamic Azad University, Babol-Branch, Babol, Iran
5- Young Researcher and Elite Club, Babol- Branch, Islamic Azad University, Babol-Branch, Babol, Iran
6- Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran ,
Abstract: (11868 Views)
ABSTRACT
Background and Objectives: Due to adverse effects of pentavalent antimonial treatment of visceral leishmaniasis using herbal medicines has received a lot of attention. In this study, we evaluated inhibitory activity of Pelargonium roseum essential oil on growth of Leishmania infantum promastigotes, and compared it with that of Glucantime.
Methods: Inhibitory effects of different concentrations (1, 2.5, 5, 10, 25, 50 and 100 µL/ml) of P. roseum essential oil on the growth of L. infantum promastigotes were studied by MTT assay after 24 and 48 hours. All the data were analyzed by one-way analysis of variance. The effect of the essential oil was later compared with that of standard dose of Glucantime.
Results: After 24 hours, different concentrations of the essential oil had no significant inhibitory effect on promastigotes of L. infantum. After 48 hours, all concentrations except for 1µL/ml showed significant anti-leishmanial activity. In addition, the lowest concentration that had significant inhibitory effect on L. infantum promastigote was 5 µL/ml.
Conclusion: The essential oil of P. roseum has favorable inhibitory activity on the growth of L. infantum promastigotes after 48 hours. This indicates the therapeutic potential of this plant for treatment of leishmaniasis.
Keywords: Leishmania infantum, Pelargonium, Promastigote, Meglumine antimoniate.
Background and Objectives: Due to adverse effects of pentavalent antimonial treatment of visceral leishmaniasis using herbal medicines has received a lot of attention. In this study, we evaluated inhibitory activity of Pelargonium roseum essential oil on growth of Leishmania infantum promastigotes, and compared it with that of Glucantime.
Methods: Inhibitory effects of different concentrations (1, 2.5, 5, 10, 25, 50 and 100 µL/ml) of P. roseum essential oil on the growth of L. infantum promastigotes were studied by MTT assay after 24 and 48 hours. All the data were analyzed by one-way analysis of variance. The effect of the essential oil was later compared with that of standard dose of Glucantime.
Results: After 24 hours, different concentrations of the essential oil had no significant inhibitory effect on promastigotes of L. infantum. After 48 hours, all concentrations except for 1µL/ml showed significant anti-leishmanial activity. In addition, the lowest concentration that had significant inhibitory effect on L. infantum promastigote was 5 µL/ml.
Conclusion: The essential oil of P. roseum has favorable inhibitory activity on the growth of L. infantum promastigotes after 48 hours. This indicates the therapeutic potential of this plant for treatment of leishmaniasis.
Keywords: Leishmania infantum, Pelargonium, Promastigote, Meglumine antimoniate.
Research Article: Original Paper |
Received: 2017/11/15 | Accepted: 2017/11/15 | Published: 2017/11/15 | ePublished: 2017/11/15
Received: 2017/11/15 | Accepted: 2017/11/15 | Published: 2017/11/15 | ePublished: 2017/11/15
References
1. Bhattacharya SK, Dash AP. Treatment of visceral leishmaniasis: options and choice. The Lancet Infec Dis. 2016; 16(2): 142-3. doi: 10.1016/S1473-3099(15)00528-9. [DOI:10.1016/S1473-3099(15)00528-9]
2. Sarkari B, Naraki T, Ghatee MA, Khabisi SA, Davami MH. Visceral Leishmaniasis in Southwestern Iran: A Retrospective Clinico-Hematological Analysis of 380 Consecutive Hospitalized Cases (1999–2014). PloS one. 2016; 11(3): e0150406. doi: 10.1371/journal.pone.0150406. [DOI:10.1371/journal.pone.0150406]
3. Mohebali M, Javadian E, Yaghoobi Ershadi M, Akhavan A, Hajjaran H, Abaei M. Characterization of Leishmania infection in rodents from endemic areas of the Islamic Republic of Iran. East Mediterr Health J. 2004; 10(4-5): 591-9.
4. Sarkari B, Hatam G, Adnani S, Asgari Q. Seroprevalence of feline leishmaniasis in areas of Iran where Leishmania infantum is endemic. Ann Trop Med Parasitol. 2009; 103(3): 275-7. doi: 10.1179/136485909X398276. [DOI:10.1179/136485909X398276]
5. Mohebali M. Visceral leishmaniasis in Iran: review of the epidemiological and clinical features. Iran j parasitol. 2013; 8(3): 348.
6. Fakhrie-Kashan Z, Arbabi M, Delavari M, Taghi-Zadeh M, Hooshyar H, Solaymani F. The effect of aqueous and alcoholic extracts of Pelarqonium roseum on the growth of Trichomonas vaginalis in vitro. KAUMS Journal (FEYZ). 2014; 18(4): 369-75.
7. Frézard F, Demicheli C, Ribeiro RR. Pentavalent antimonials: new perspectives for old drugs. Molecules. 2009; 14(7): 2317- 36. doi: 10.3390/molecules14072317. [DOI:10.3390/molecules14072317]
8. Hejazi H, Layeghi Ghale S, pirali kheirabadi Kh. Cytotoxic effect of essential oil of Artemisia Siberi and Pelargonium roseum on Leishmania major Promastigotes. J Shahrekord Univ Med Sci. 2015; 17(5): 13-22.
9. Carmen G, Hancu G. Antimicrobial and Antifungal Activity of Pelargonium roseum Essential Oils. Adv Pharm Bull. 2014; 4(Suppl 2): 511-514. doi: 10.5681/apb.2014.075.
10. Piralikheirabadi KH, Razzaghi-Abyaneh M, Halajian A. Acaricidal effect of Pelargonium roseum and Eucalyptus globulus essential oils against adult stage of Rhipicephalus (Boophilus) annulatus in vitro. Vet Parasitol. 2009; 162(3-4): 346-9. doi: 10.1016/j.vetpar.2009.03.015. [DOI:10.1016/j.vetpar.2009.03.015]
11. Kozan E, Akkol EK, Süntar I. Potential anthelmintic activity of Pelargonium endlicherianum. Ethnopharmacol. 2016; 187: 183- 6. doi: 10.1016/j.jep.2016.04.044. [DOI:10.1016/j.jep.2016.04.044]
12. Oryan A. Plant-derived compounds in treatment of leishmaniasis. Iran J Vet Res. 2015; 16 (1): 1.
13. Hotez PJ, Savioli L, Fenwick A. Neglected Tropical Diseases of the Middle East and North Africa: Review of Their Prevalence, Distribution, and Opportunities for Control. PLoS Negl Trop Dis. 2012; 6(2):e1475. doi: 10.1371/journal.pntd.0001475. [DOI:10.1371/journal.pntd.0001475]
14. Machado M, Dinis A, Santos-Rosa M, Alves V, Salgueiro L, Cavaleiro C. Activity of Thymus capitellatus volatile extract, 1, 8-cineole and borneol against Leishmania species. Vet parasitol. 2014; 200(1): 39-49. [DOI:10.1016/j.vetpar.2013.11.016]
15. Colares AV, Almeida-Souza F, Taniwaki NN, Souza CdSF, da Costa JGM, Calabrese KdS. In vitro antileishmanial activity of essential oil of Vanillosmopsis arborea (Asteraceae) baker. J Evid Based Complementary Altern Med. 2013; 4: 7-11. doi: 10.1155/2013/727042. [DOI:10.1155/2013/727042]
16. Boukhatem MN, Kameli A, Ferhat MA, Saidi F. Mekarnia M. Rose geranium essential oil as a source of new and safe anti-inflammatory drugs. Libyan J Med. 2013; 8: 22520. doi: 10.3402/ljm.v8i0.22520. [DOI:10.3402/ljm.v8i0.22520]
17. Rodrigues IA, Azevedo MM, Chaves FC, Bizzo HR, Corte-Real S, Alviano DS. In vitro cytocidal effects of the essential oil from Croton cajucara (red sacaca) and its major constituent. BMC Complementary and Alternative Medicine 2013; 13: 249. DOI: 10.1186/1472-6882-13-249. [DOI:10.1186/1472-6882-13-249]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Enamad
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.