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Background and Objective: Knee osteoarthritis is one of the most common causes of musculoskeletal disability. This study was performed to determine the effect of endurance training and glucosamine consumption on Insulin-Like Growth Factor 1 (IGF-1) and Insulin-Like Growth Factor Binding Protein 3 (IGFBP-3) gene expression in knee tissue of mice with osteoarthritis.
Methods: This experimental study was performed on 30 adult male Wistar rats. Induction of osteoarthritis of the knee was performed by surgery. The animals were randomly allocated into 5 groups and each group consisted of 6 mice. Groups include: Control, Saline, glucosamine, train and train-glucosamine. The training program for eight weeks, three sessions per week and included 29-25 minutes running on a treadmill at a speed of 15 m/ min for the first week, and each week one meter per minute was added to reach 22 m/min for the eighth week. All animals followed 12-hour fasting and 48 hours after the last session of the session, anesthetized with intraperitoneal injection of Ketamine and Xylosin. The expression of IGF-1 and IGFBP-3 of cartilage was measured by Real Time PCR.
Results: Expression of IGF-1 and IGFBP-3 increased to be 22% and 6% in control group compared to Saline group. Also, level of IGF-1 in exercise groups, glucosamine and exercise-glucosamine group was significantly increased compared to control group (P<0.05). The gene expression of IGFBP-3 in the exercise-glucosamine group was significantly increased compared to the control group (P<0.05).
Conclusion: Probably Chondrocytes secrete higher levels of IGF-1 during tissue damage that causes the structural synthesis of collagen 2 and proteoglycans and Finally, endurance training with consumption of glucose amine increases the expression of IGFBP-3 gene.

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Background and Objective: Unhealthy lifestyle is the main cause of morbidity, mortality and preventable complications in the elderly. Performing health promotion behaviors are one of the most effective factors in maintaining and improving health. This study was done to determine the health promotion lifestyles in the elderly people.
Methods: This descriptive-analytical study was performed on 110 elderly people aged 60-88 years referred to teaching and medical centers in Gorgan, north of Iran during 2019. Data was collected using Health Promotion Lifestyle Profile 2 (HPLP-II) questionnaire. This questionnaire includes six dimensions of spiritual growth, responsibility for health, interpersonal relationships, stress management, physical activity and nutrition for evaluation health promotion behaviors status. The total score range of the instrument ranges from 52 to 208. A score of 52-103 was considered low level of compliance with health promotion behaviors and a score of 104-155 and scores above 156 was considered intermediate and high level, respectively.
Results: The mean±SD of health promotion lifestyle was 143.52±17.36. There was a significant relationship between lifestyle and demographic characteristics (P<0.05). Spiritual growth and interpersonal relationships had the highest score among the six dimensions of health promotion behaviors.
Conclusion: Health promotion lifestyle in the elderly was low in terms of physical activity and stress management.

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Background and Objective: Developmental disorder and environmental factors are considered as the main causes in postural abnormalities in children and adolescents. This study was done to evaluate the role of BMI, physical activity and the use of electronic device in the status of trunk abnormalities in male adolescents.
Methods: This descriptive-analytical study was conducted on 480 boys of 13 to 15 years old in Golestan province, northern Iran. Trunk abnormalities were measured using a spinal mouse. Physical activity was assessed using a standard physical activity questionnaire. Use of electronic device was measured using the form of rate of use of electronic device.
Results: Kyphosis abnormality in 13, 14 and 15 years-old male adolescents were reported 70%, 65% and 75%, respectively. In addition, 60% of 13 years-old, 40% of 14-years-old, and 50% of 15-years-old adolescents had lordosis abnormality. There was a direct and significant correlation between kyphosis abnormalities with BMI and using electronic device as well as a reverse significant correlation between lordosis abnormality and BMI was demonstrated.
Conclusion: This study indicated that trunk abnormalities are relatively common in male adolescents in Golestan province, northern Iran. Also, BMI and the use of electronic device can also affect these abnormalities.

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Selenium is an essential mineral found naturally in soil, water, and some foods. Today, selenium, as an antioxidant, is one of the vital elements in the human body. Although the human body needs a small amount of selenium daily, recent studies have shown that the same small amount of selenium is essential for maintaining healthy body function. It is challenging to choose the right dose of selenium. According to several studies in this present review, a daily supplement of 31.5-200 micrograms seems beneficial since the amount of selenium in various diseases is less than the amount in healthy people. The articles published in PubMed, Web of Science, Google Scholar and SCOPUS databases were screened and collected based on the keywords including selenium, oxidant, antioxidant, diabetes, cancer, toxicity, heart disease and liver disease. These keywords were chosen because of the antioxidant role of selenium as well as the prominent role of oxidation in the progression of these diseases. 150 articles published over a period of 20 years from 2000 to 2020 were used in this study. Articles about nano-seleniums, non-dietary supplements, and diseases caused by the underlying disease of metabolic syndrome were excluded and finally 121 articles were included in the study. A limited number of studies show that patients with multiple disorders may need to take higher doses of selenium (82.4 to 200 micrograms) than healthy people. In this study, selenium's antioxidant role in chronic metabolic disorders, including hyperlipidemia and hyperglycemia, and some cancers, were investigated. Also, the clinical significance of selenium deficiency in metabolic diseases, as well as clinical and experimental observations on the effect of selenium dietary supplements in the treatment of chronic metabolic diseases such as diabetes and atherosclerosis, and cancers, are briefly mentioned. Besides, the recommended toxicity and dose of selenium have been discussed.

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Background and Objective: Using chewing tobacco is one of the most important health problems in ports of the southern coast of Iran. The prevention of this problem requires identifying the factors affecting this phenomenon. The aim of this study was to determine the prevalence of chewing tobacco and related factors among adolescents in South- East of Iran (2018).
Methods: This descriptive-analytical study was conducted on 204 male high school students through multistage sampling in two the ports of Konarak and Chabahar located in south - east of Iran during March to June 2018. The data collection tool was a self-administered standardized questionnaire including items about knowledge, attitude and prevalence of chewing tobacco among participants, intimate friends and family members of the study population.
Results: Prevalence of chewing tobacco in life time and current use (pervious 30 days) was 71.8% and 43.1%, respectively. Supari was the main chewing tobacco were used (80.8%) by participants. The negative attitudes about chewing tobacco was a protective factor (OR=0.41) and easy access (OR=6.88), using by family members (OR=2.28), and close friends (OR=3.28) were the most important risk factors.
Conclusion: The prevalence of chewing tobacco was alarming in male high school students in south - east of Iran.

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Background and Objective: Bisphenol A (BPA) is the monometer of epoxy resins and polycarbonate plastics that plays an important role in the pathogenesis of cardiovascular diseases. This study was done to evaluate the effect of aerobic exercises on oxidative stress indices in heart tissue of male Wistar rats after poisoning with Bisphenol A.
Methods: In this experimental study, 20 adult male Wistar rats were allocated into four groups including control, aerobic exercise, BPA and BPA + aerobic exercise. Animals in control and aerobic exercise groups were receieved olive oil and the BPA (100 μg/kg) intraperitoneally, respectively. Animals in BPA + aerobic training group were treated with BPA (100 μg/kg) intraperitoneally for 30 days. Exercise program consisted of 25-64 minutes of running with 57-75% oxygen consumption, 5 sessions per week for two months. Indicators of oxidative stress in heart tissue including Gpx, SOD, TAC, MDA, CAT and AOPP were evaluated.
Results: BPA intoxication induced a significant increase in the activity of SOD , GPX enzymes, AOPP and MDA levels and  reduction  in CAT enzyme activity and TAC level in compared to control group (P<0.05). While aerobic exercise could diminish the oxidative stress due to BPA intoxication. Aerobic exercise reduced the activity of the GPX, SOD and increased catalase activity in rats with BPA intoxication (P<0.05). Also, level of MDA and AOPP significantly reduced in in aerobic exercise group in comparison with with BPA intoxication (P<0.05).
Conclusion: Aerobic exercise activity has moderating effects on BPA intoxication in cardiac oxidative stress. Aerobic exercise can increase the amount of antioxidant enzymes that play an important role in modulating oxidative stress induced by BPA.

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Background and Objective: Hypertension is associated with changes in the structure and function of the vessels. This study was done to evaluate the effect of aerobic training with Garlic consumption on matrix metalloproteinase-3, 9 (MMP-3, MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in obese postmenopausal women with hypertension.
Methods: This double-blind clinical trial study was done on 36 obese postmenopausal women with hypertension. Participants were randomly divided into 4 groups including control group, Garlic consumption, aerobic training and training with Garlic consumption. Subjects in training groups participated in a progressive aerobic training for eight weeks, three sessions a week (55% to 65% of the reserved heart rate and for 30 to 55 min). Garlic and training with Garlic groups consumed 1000 mg of garlic supplement for eight weeks (after breakfast and dinner). Two days before and after the protocol, blood samples were taken in fasting state.
Results: The level of MMP-3 and MMP-9 were significantly decreased in Garlic, aerobic training and training with Garlic groups in compared to the control group (P<0.05). Also, the level of MMP-3 in the training with Garlic was significantly reduced in compared to the training and the Garlic groups (P<0.05). MMP-9 in the training with Garlic was significantly reduced in compared to the Garlic group (P<0.05). However, TIMP-1 level were significantly increased in the Garlic, training and training with Garlic in comparison with control group (P<0.05).
Conclusion: Aerobic training alone and in combination with Garlic affect on extracellular matrix thorough reduction of MMP-3, MMP-9 and increasing TIMP-1, improves systolic blood pressure in obese postmenopausal women with hypertension.

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Background and Objective: Gilbert's syndrome is a relatively common genetic disorder, which is caused by defection in uridine diphosphate glucuronosyl transferase enzyme. The indirect bilirubin increases in this syndrome, although the function of the liver is normal. Gilbert's syndrome can be seen in 3 to 10% of different populations. According to the differences in ethnic groups in Golestan Province, no studies have been conducted on the prevalence of the syndrome in the province, so far.This study was conducted to determine the prevalence of suspected Gilbertʼs syndrome in Golestan province in north of Iran.
Methods: This descriptive-analytical study was performed on 1664 subjects with 18-45 years old referring to rural and urban health centers in Golestan province, North of Iran during 2014. Liver function tests were normal in subjects. Fasting blood samples were taken from each subject and total bilirubin was tested. People with a total bilirubin of more than 1.5 mg/dl were treated with a single oral dose of rifampin 600 mg. After taking rifampicin, the individuals with an indirect bilirubin level of more than 1.3 mg/dl were found suspected of Gilbert’s syndrome.
Results: The prevalence of suspected Gilbert's syndrome was 5.8%. Moreover, suspected Gilbert’s syndrome was more common in males than females (10% in males and 4.3% in females) (P<0.05). According to ethnicity, the prevalence of suspected Gilbert's syndrome was 5.4%, 5.4%, and 6.8% in Sistani, Fars and Turkmen subjects, respectively. This difference was not significant. The prevalence of suspected Gilbert's syndrome in three ethnicities was higher in males than females and it was statistically significant in Sistani and Fars ethnicities (P<0.05) but not significant in Turkmen ethnicity.
Conclusion: Suspected cases of Gilbert's syndrome were more common in men than women, and more prevalent in the Turkmen ethnic group.

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Background and Objective: Exercise in cardiac patients after coronary artery bypass grafting (CABG) improves cardiovascular function by increasing the capillary density of the myocardium. Platelet-derived growth factor and stromal cell-derived factor-1 play an important role in angiogenesis and cell proliferation and differentiation. This study was performed to determine the effect of eight weeks of combined training on plasma levels of Platelet-derived growth factor (PDGF) and stromal cell-derived factor-1 (SDF1) in CABG patients.
Methods: In this clinical trial study, 24 patients who underwent coronary artery bypass grafting operation were selected using convenience and purposeful sampling method and randomly divided into two combined training and control groups. Subjects in combined training group were received aerobic and resistance training protocols for eight weeks, three sessions per week. Before and after training program, body composition assessments and blood sampling were performed to measure Platelet-derived growth factor (PDGF) and stromal cell-derived factor-1 (SDF1). ELISA method was used to measure PDGF and SDF1.
Results: The level of PDGF was significantly increased in intervention group in compared to control group (P<0.05). Although the level of SDF1 in the intervention group increased in intervention group in compared to control group; but this increase level of SDF1 was not significant.
Conclusion: This study showed that the eight weeks of combined rehabilitation training can promote desirable physiological adaptations in cardiovascular health by increasing the plasma levels of PDGF.

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Background and Objective: Foreign body aspiration is the cause of death in 7% of children under two year’s olds. Children’ molars teeth do not erupt and under devedoled ability to swallow food and generally likely to be distracted, playing or ambulatory while eating. This study was performed to determine the frequency of foreign body aspiration in children under ten years old in north of Iran.
Methods: This descriptive study was done on 50 children (21 boys, 29 girls) less than ten years old with the final detection of foreign body aspiration hospitalized in Taleghani hospital in Gorgan, north of Iran during 2016-17. Demographic characteristics, results of physical examination, radiography and bronchoscopy recorded in the file and the radiology report was collected by a pulmonologist and radiologist.
Results: The common age of foreign body aspiration was between 1-3 years (58%) followed by over 3 years (24%) and under 1 years old (18%). Symptoms of patients with foreign body aspiration were including cough (64%), rhonchi (52%), respiratory distress (32%), wheezing (32%), choking (30%). Common physical examination findings were wheezing (40%), unilateral pulmonary sound (34%), stridor (20%), and rhonchi (6%). The most common findings in Chest X-Rays were unilateral air trapping (48%) in right lung, left mediastan shift (30%), right lung atelectasia (20%), pneumonia (16%). Just in one case (2%) was found the body of radio opac in right lung and in 32% of cases, the graghs were normal. In 70% of patients less 24 hours, in 26% between 1-15 Dayes and in 4% of cases more than 15 Dayes were taken to medical centers. The most common foreign body was nuts (32%) followed by plastic objects (26%), aspiration by sunflower seeds (14%) and beens (10%) and other foreign objects (18%).
Conclusion: The most common clinical signs of foreign body aspiration were cough, wheezing and right lung pneumonia.

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Background and Objective: The incidence of tuberculosis in Golestan province in northern Iran is 26.7 cases in 100000 persons. Lack of energy and nutrients is one of the reasons for the increased risk of tuberculosis. Malnutrition is an important cause of high mortality in the Tuberculosis (TB) population. With decreasing level of visceral proteins, anthropometric indices, and micronutrients increase the risk of mortality. This study was performed to determine the status of nutritional status of patients with Tuberculosis in Gonbad-e-Kavous, north of Iran during 2014.
Methods: This descriptive-analytical study was performed on 45 pulmonary tuberculosis patients with mean age of 57±20 living in Gonbad city, north of Iran during 2014. Demographic information and history of tuberculosis, body mass index, albumin, hemoglobin, total protein and blood cell count and food intake were recorded for each patient.
Results: 58% of patients were women. 52% of patient was resident in rural area. There was a significant difference in terms of body mass index and previous history of tuberculosis in patients with different disease intensities. Among patients with different disease intensities, the difference in the distribution of anemia severity was not significant. There was no significant difference between the two sexes in terms of disease severity distribution. According to the fitted regression model, the age variable, even in the presence of other variables, had a significant effect on disease severity (P<0.05).
Conclusion: With increasing disease severity, patients' BMI decreases and the severity of malnutrition and anemia increases. The severity of the disease increases with age. Tuberculosis patients need nutritional support.

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Background and Objective: Colorectal cancer is one of the most common cancers and considered to be leading causes of cancer death in the world. One of the problems with this type of cancer is its inability to be detected in early stage. The aim of present study was to evaluate the correlation between the serum levels of Fucosyltransferase-4 and galactin-3 in patients with early-stage of colorectal cancer.
Methods: This case-control study was done on 40 patients with early stages of colorectal cancer and 40 healthy subjects. ELISA method was used to measure the serum levels of Fucosyltransferase-4 and galactin-3. To examine the correlation between the variables, correlation test and Pearson correlation coefficient index were used.
Results: Fucosyltransferase-4 and galactin-3 levels were higher in patients with early-stage of colorectal cancer compared to healthy subjects. This difference was not significant. In patients with colorectal cancer, there was a significant relationship between the level of Fucosyltransferase-4 and galactin-3 (r=0.71, P=0.01).
Conclusion: Simultaneous measurement of Fucosyltransferase-4 and galactin-3 is useful in identifying early-stage of colorectal cancer as a non-invasive laboratory method.

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Background and Objective: Spastic diplegia cerebral palsy is a movement disorder originating from brain injury before, during, or immediately after birth. Exercise plays an important role in motor recovery. This study was performed to determine the effect of high-intensity circuit training on balance and activity daily living in children with cerebral palsy (CP).
Methods: In this clinical trial, 34 children with cerebral palsy were randomly divided into two groups of 17 including occupational therapy (control group) and circuit training group with occupational therapy as interventional group. Berg balance scale and activity scale for kids were used to assess changes in balance and activity of daily living before and after 12 weeks of exercise, respectively. High-intensity circuit training consisting of 3 rounds and each round consisting of 6,
30-second stations and a 30-second break between stations, which were performed three times a week for 12 weeks. Occupational therapy was performed one session per week.
Results: The rate of balance and activities of daily living and its components in the circuit training group with occupational therapy showed a significant improvement compared to control group (P<0.05).
Conclusion: Circuit trainings with occupational therapy can further improve the performance of balance and activity of daily living in children with Spastic diplegia cerebral palsy compared to occupational therapy.

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Background and Objective: Stroke and cerebral ischemia are the second leading causes of death in the world. Currently, there are limited therapeutic interventions for patients with ischemia / reperfusion. This study was performed to determine the protective effect of aerobic exercise and adenosine on changes in inflammation mediators after transient ischemia of common carotid arteries in male Wistar rats.
Methods: In this experimental study, fifty male Wistar rats were randomly allocated into to 5 groups: control, cerebral ischemic control, aerobic exercise + cerebral ischemia, adenosine + cerebral ischemia and aerobic exercise + adenosine + cerebral ischemia. Ischemia was performed by blocking the common carotid artery for 45 minutes after a period of exercise and injection of adenosine. Neuronal structure was examined by Nissel tissue staining. The expression of NGF and Glutamate genes were measured in CA1 region of hippocampal tissue samples.
Results: Cell death was increased in neurons in the CA1 region of the hippocampus in the ischemia / reperfusion group, While a significant reduction in cell death in the adenosine + ischemia / reperfusion and aerobic exercise + ischemia /reperfusion groups was due to adenosine administration and aerobic exercise (P<0.05). NGF and glutamate gene expression in the adenosine + ischemia/reperfusion and adenosine + aerobic exersice + ischemia/reperfusion groups significantly increased and reduced compared to the ischemia/reperfusion control, respectively (P<0.05).
Conclusion: Co-administration of adenosine combined with aerobic exercise increase the protective effect of aerobic exercise on improving the neuronal damage after ischemic / reperfusion.

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Background and Objective: One of the most important attempts in crisis is the nutrition management of affected people during and following the disaster. In March 2019, floods occurred in towns and villages on the outskirts of the Gorganrood River in Golestan province, northern Iran. This study conducted to explain the challenges of nutrition management in the flood crisis of Golestan province.
Methods: In this qualitative study, 31 people affected by floods, urban and provincial managers and experts involved in crisis management were selected by purposive sampling method. Data were collected using an interview guide and semi-structured in-depth interviews. Data were recorded and transcribed and analyzed using the content analysis method and Atlas.ti 8 software.
Results: The managerial challenges included planning, organization, coordination, control, and evaluation. The executive challenges contained food preparation, food distribution, and monitoring. The other challenges included political, economic, cultural, and ecological challenges.
Conclusion: Most of the respondents emphasized the irregularity and confusion in nutrition management during the flood and considered it as a result of managerial challenges, executive challenges, political, economic, cultural and ecological challenges.

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Background and Objective: Binding of antibiotics to nanoparticles increases the antibacterial potential of nanoparticles and antibiotics. This study was performed to determine the antibacterial and hemolytic effect of zinc / ferrite / cellulose nanocomposite (ZnFe2O4 @ Cell) (single nanoparticle), zinc / ferrite / cellulose nanocomposite was aminated with 3-aminopropyltriethoxysilane (APTES) with the name of ZnFe2O4@Cell@APTES (Coated nanocomposite) and ZnFe2O4@Cell@APTES@Van nanocomposite (coated nanocomposite bound to vancomycin) against gram-negative bacteria Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa) and gram-positive bacterium Staphylococcus aureus (S. aureus).
Methods: In this descriptive study, antibacterial-activity was evaluated by broth macro dilution method. Minimum inhibitory concentration (MIC) and minimum lethal concentration (MBC) were determined for E. coli, S. aurous and P. aeruginosa. The hemolytic activity of nanoparticles was investigated by colorimetric method.
Results: Nanoparticles did not have hemolytic activity. ZnFe2O4@Cell and ZnFe2O4@Cell@APTES@Van did not have a significant antibacterial effect against gram-positive and gram-negative bacteria, and vancomycin binding resulted in antibacterial-activity. ZnFe2O4@Cell@APTES@Van inhibited the growth of Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa. The growth of E. coli was reduced to 85% at a concentration of 0.4 mg/ml and a concentration of 0.1 mg nanoparticles completely prevented the growth of P. aeruginosa. The growth of gram-positive S. aureus bacteria at a concentration of 0.3 mg/ml nanoparticles was completely stopped.
Conclusion: Vancomycin-modified nanocomposite has antibacterial-activity against both gram-positive and gram-negative bacteria and has the potential to overcome the antibiotic resistance of bacteria.

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Background and Objective: Coronary artery disease (CAD) is one of the major cardiovascular diseases that affect the global population. This study was performed to evaluate the electrocardiogram (ECG) changes of patients before and after coronary artery bypass graft (CABG) surgery.
Methods: This descriptive-analytical study was performed on 100 patients (71 males and 29 females) in the age range of 37 to 78 years (mean age of 57.87±8.14 years) by census method undergoing CABG surgery in Amir Al-Momenin Kordkoy Hospital, north of Iran during the second half of 2020. Data including age, sex, and results of ECG changes, including changes in heart rate and pulse rate (PR) the day before surgery, the day of hospitalization, 4 hours after surgery, 18 hours after surgery, one day, and two days after surgery was recorded in a questionnaire.
Results: The highest mean heart rate and PR were observed at 4 hours after surgery and then heart rate and PR were reduced and on the second day, heart rate and PR were lower than  the day of hospitalization (P<0.05).
Conclusion: Considering the significant changes in ECG after surgery, careful examination and monitoring of patients, especially high-risk individuals led to early detection of significant changes in patients and reduced subsequently complications in patients with appropriate and timely treatment.

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Background and Objective: brucellosis is one of the most common zoonosis diseases. Brucellosis is a systemic disease and affected several organs and tissue in human. This study was performed to determine the knowledge, attitude and practice about brucellosis control among livestock farmers.
Methods: This descriptive-analytic study, was performed on 300 rural literate livestock farmers in BuinZahra, Qazvin Iran during 2019. The knowledge, attitude, and practice, the related factors and demographic characteristics such as age, education level and family count were evaluated by structured six-part questionnaire including 30 questions.
Results: The mean age of the subjects was 41.3±16.4 years. Although 90.7% had heard of brucellosis, only 14% had previous trained. There was a positive attitude about the use of methods to reduce the risk of infection in the family and the seriousness of the issue of animal brucellosis in 19% and 64% of cases, respectively. About 94.7% were attended to veterinarian in suspected cases and 96.7% examined the new animals. 97% were uses of new cheese and milk but the milk was boiled by 89.3%. The mean knowledge was 5.3 ranging from 0 to 8 points in them. There was a statistically significant difference in the knowledge score of individuals according to the information source (P<0.05). Knowledge score and number of household members had positive correlation (r=0.135, P=0.019).
Conclusion: There is medium-level knowledge about brucellosis in livestock farmers. The attitude and some actions (such as dealing with infected livestock) needed to be improved. The probable reason is the high cost of behavior modification in some areas, which has provided the gap between knowledge, attitude and behavior in this area.

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Background and Objective: The presence of many synthetic dyes in aqueous solutions can cause carcinogenesis and mutagenicity and affect human health. Reactive Red 198 is one of the types of azo dyes with complex structure, toxic, carcinogenicity, mutagenicity properties, and tolerable in the environment, which is discharged to the environment through the sewage of textile industries. This study was performed to determine the mineralization of 198 reactive red dyes by the hybrid advanced oxidation process UV/US/H₂O₂/O₃ from colored wastewater using central composite design.
Methods: In this descriptive-analytical study several factors affecting on dye mineralization process including the presence of ozone gas and ultraviolet rays (UV) as well as the initial dye concentration, Ultrasound (US), contact time, pH, and hydrogen peroxide were investigated. Experimental design and optimization were performed by design of experiment software using central composite design and its optimal conditions were determined.
Results: The maximum dye degradation efficiency of 100% was performed under optimal conditions including initial dye concentration of 200 mg/L, reaction time of 34 minutes, hydrogen peroxide concentration of 27 mg/L and pH=9 in the presence of ozone gas flow, ultraviolet rays and ultrasonic waves. Also, the influence of factors on dye removal including ozone, UV, initial dye concentration, US, contact time, pH, and peroxide were  58.8%, 19.3%, 2.3%, 1.5%, 1.1%, 0.6 %and 0.2 % ,respectively.
Conclusion: UV/US/H₂O₂/O₃ hybrid process with advantages such as performance and high speed can be recommended for wastewater treatment in various industries.

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Background and Objective: Since the onset of the COVID-19 (Corona Virus Disease 2019) pandemic, several challenges have been proposed to the disease and the causing viral agent. Accurate and rapid diagnosis of the virus is essential to control the spread and progression of the disease. Choosing a suitable sample in different phases of the disease will reduce the false-negative results. This study was performed to identify the SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) genome in the blood samples of COVID-19 patients.
Methods: This descriptive-analytical study was performed by census method on 100 whole blood samples of patients (50 recovery and 50 deceased) with a definitive diagnosis of COVID-19 (positive Real-Time RT-PCR test of nasopharyngeal swab samples) admitted to Shahid Sayyad Shirazi educational and medical center in Gorgan during 2020-21. Clinical and laboratory findings were compared in the two groups of patients. The viral nucleic acid was extracted from the whole blood samples of the patients, and the presence of the virus genome was investigated using primer and probes via the Real-Time RT-PCR method.
Results: The age of the recovered patients (49.06±15.1 years) was significantly was lower than deceased patients (58.3±12.4 years) (P<0.05). Clinical symptoms including cough, shortness of breath, sputum secretion, and vomiting in deceased patients were significantly more than recovery group (P<0.05). The lymphocytes count and platelet level in the deceased group were lower than in the recovered group. Level of lactate dehydrogenase (LDH) was higher in the deceased group in compare to recovered group (P<0.05). The virus genome identified in the blood samples of 7 patients (3 recovered and 4 deceased), which had no significant relationship with the outcome of the disease.
Conclusion: The use of blood samples for the diagnosis of COVID-19 is not appropriate.