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:: Volume 22, Issue 2 (6-2020) ::
J Gorgan Univ Med Sci 2020, 22(2): 68-74 Back to browse issues page
Effect of Pomegranate peel extract on expression of angiogenesis stimulating gene (VEGF) in A2780 cell line of ovarian cancer
Fateme Zamani Esmati1 , Narges Nikoonahad Lotfabadi 2, Bibi Fatemeh Haghiralssadat3
1- M.Sc. in Cellular and Molecular Biology, Department of Biology, Faculty of Sciences, Science and Arts University, Yazd, Iran
2- Ph.D in Develpmental Biology, Assistant Professor, Department of Biology, Faculty of Sciences, Science and Arts University, Yazd, Iran , nikounahad_1976@yahoo.com
3- Ph.D of Nanobiotechnology, Assistant Professor, Department of Advanced Medical Sciences and Technology, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Abstract:   (4988 Views)
Background and Objective: Ovarian cancer, also known as “The Silent Killer,” is one of the most dangerous cancers for women, which often diagnosed late and incurable. On the other hand, conventional therapies currently have limitations, failures and various side effects. This study was performed to determine the effect of pomegranate peel extract on the expression of angiogenesis stimulating gene (Vascular Endothelial Growth Factor: VEGF) by culturing A2780 cell line of ovarian cancer.
Methods: In this descriptive-analytical study, pomegranate peel extract was prepared and then ovarian cancer cells (A2780 cell line) were exposed to different concentrations of pomegranate peel extract (500, 250, 100, 75, 50, 25 and 10 µg/ml) for 48, 24 and 72 hours. Also, the survival rate of the cells was tested by MTT assay and VEGF gene expression was evaluated using RT-PCR.
Results: Pomegranate peel extract concentration of 500 µg/ml reduced the survival rate to 18% in 72 hours (P<0.05). At concentrations of 200, 100 and 50 µg/ml of pomegranate peel extract, the expression of VEGF reduced by 7%, 16% and 19%, respectively, which was significant compared to the control group (P<0.05).
Conclusion: Pomegranate peel extract, due to its numerous compounds and significant antioxidant properties, is likely to reduce metastasis and malignant manifestations by reducing the expression of the angiogenesis agent.
Keywords: Pomegranate [MeSH], Ovarian cancer [MeSH], Survival rate [MeSH], VEGF [MeSH], Angiogenesis [MeSH]
Article ID: Vol22-24
Full-Text [PDF 342 kb]   (11080 Downloads)    
Type of Study: Original Articles | Subject: Oncology
References
1. Marjani M. [Epidemiology and risk factors of ovarian cancer]. Iran J Obstet Gynecol Infertil. 2010; 5(3): 67-73. [Article in Persian]
2. Noori-Daloii MR, Rashvand Z. [Molecular Genetics and Gene Therapy in Ovarian Cancer]. Horizon Med Sci. 2010; 16(3): 5-19. [Article in Persian]
3. Folkman J. Angiogenesis and breast cancer. J Clin Oncol. 1994 Mar; 12(3): 441-43. DOI: 10.1200/jco.1994.12.3.441
4. Banerjee S, Kaye SB. New Strategies in the Treatment of Ovarian Cancer: Current Clinical Perspectives and Future Potential. Clin Cancer Res. 2013 Mar; 19(5): 961-68. DOI: 10.1158/1078-0432.CCR-12-2243
5. Aminimoghaddam S, Norouzi S. [Ovarian failure due to cancer treatment and fertility preservation options]. Tehran Univ Med J. 2016; 74(1): 1-8. [Article in Persian]
6. Mousavi A, Karimi-Zarchi M, Behtash N, Mokhtari-Gorgani M, Mehrdad N, Rouhi M, et al. [The effect of consolidation treatment with intraperitoneal carboplatin in advanced epithelial ovarian cancers]. Tehran Univ Med J. 2014; 72(4): 215-21. [Article in Persian]
7. Sreekumar S, Sithul H, Muraleedharan P, Azeez JM, Sreeharshan S. Pomegranate Fruit as a Rich Source of Biologically Active Compounds. Biomed Res Int. 2014; 2014: 686921. DOI: 10.1155/2014/686921
8. Masoumi Moghaddam S, Amini A, Morris DL, Pourgholami MH. Significance of vascular endothelial growth factor in growth and peritoneal dissemination of ovarian cancer. Cancer Metastasis Rev. 2012 Jun; 31(1-2): 143-62. DOI: 10.1007/s10555-011-9337-5
9. Sarkhosh A, Zamani Z, Fatahi R, Hassani ME, Wiedow C, Buck E, et al. Genetic diversity of Iranian soft-seed pomegranate genotypes as revealed by fluorescent-AFLP markers. Physiol Mol Biol Plants. 2011 Jul; 17(3): 305-11. DOI: 10.1007/s12298-011-0070-x
10. Zamani Z. [Characteristics of Pomegranate Cultivars Grown in Saveh of Iran]. MSc Thesis. University of Tehran. 1990. [Persian]
11. Song B, Li J, Li J. Pomegranate peel extract polyphenols induced apoptosis in human hepatoma cells by mitochondrial pathway. Food Chem Toxicol. 2016 Jul; 93: 158-66. DOI: 10.1016/j.fct.2016.04.020
12. Loizzi V, Del Vecchio V, Gargano G, De Liso M, Kardashi A, Naglieri E, et al. Biological Pathways Involved in Tumor Angiogenesis and Bevacizumab Based Anti-Angiogenic Therapy with Special References to Ovarian Cancer. Int J Mol Sci. 2017 Sep; 18(9) pii: E1967. DOI: 10.3390/ijms18091967
13. Choi HJ, Armaiz Pena GN, Pradeep S, Cho MS, Coleman RL, Sood AK. Anti-vascular therapies in ovarian cancer: moving beyond anti-VEGF approaches. Cancer Metastasis Rev. 2015 Mar; 34(1): 19-40. DOI: 10.1007/s10555-014-9538-9
14. Keshavarzi M, Esfandani Bozchaloyi S, Kiarostami K. [Pharmagnosy, total phenolic and flavonoid contents and investigation on antioxidant properties of stem and leaf extracts of 6 Stellaria species and two related genera in Iran]. Applied Biology. 29(1): 143-58. [Article in Persian]
15. Yang H, Dou QP. Targeting apoptosis pathway with natural terpenoids: implications for treatment of breast and prostate cancer. Curr Drug Targets. 2010 Jun; 11(6): 733-44.
16. Ajikumar PK, Xiao WH, Tyo KE, Wang Y, Simeon F, Leonard E, et al. Isoprenoid pathway optimization for Taxol precursor overproduction in Escherichia coli. Science. 2010 Oct; 330(6000): 70-74. DOI: 10.1126/science.1191652
17. Gil MI, Tomás-Barberán FA, Hess-Pierce B, Holcroft DM, Kader AA. Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. J Agric Food Chem. 2000 Oct; 48(10): 4581-89. DOI: 10.1021/jf000404a
18. Mehta R, Lansky EP. Breast cancer chemopreventive properties of pomegranate (Punica granatum) fruit extracts in a mouse mammary organ culture. Eur J Cancer Prev. 2004 Aug; 13(4): 345-48. DOI: 10.1097/01.cej.0000136571.70998.5a
19. Amin AR, Kucuk O, Khuri FR, Shin DM. Perspectives for cancer prevention with natural compounds. J Clin Oncol. 2009 Jun; 27(16): 2712-25. DOI: 10.1200/JCO.2008.20.6235
20. Faria A, Calhau C. The bioactivity of pomegranate: impact on health and disease. Crit Rev Food Sci Nutr. 2011 Aug; 51(7): 626-34. DOI: 10.1080/10408391003748100
21. Askari M, Nikoonahad Lotfabadi N. [Evaluation of niosomal nano-carriers capabilities on toxicity preservation and delivery of pomegranate peel extract in cell culture conditions (MCF-7 cell line of breast cancer)]. Daneshvar. 2018; 26(5): 9-20. [Article in Persian]
22. Lee CJ, Chen LG, Liang WL, Wang CC. Anti-inflammatory effects of Punica granatum Linne invitro and in vivo. Food Chemistry. 2010; 118(2): 315-22. DOI: 10.1016/j.foodchem.2009.04.123
23. Dana N, Javanmard SH, Rafiee L. Antiangiogenic and antiproliferative effects of black pomegranate peel extract on melanoma cell line. Res Pharm Sci. 2015 Mar-Apr; 10(2): 117–24.
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Zamani Esmati F, Nikoonahad Lotfabadi N, Haghiralssadat B F. Effect of Pomegranate peel extract on expression of angiogenesis stimulating gene (VEGF) in A2780 cell line of ovarian cancer. J Gorgan Univ Med Sci 2020; 22 (2) :68-74
URL: http://goums.ac.ir/journal/article-1-3609-en.html


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Volume 22, Issue 2 (6-2020) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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