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:: Volume 18, Issue 1 (3-2016) ::
J Gorgan Univ Med Sci 2016, 18(1): 23-29 Back to browse issues page
Effect of Diazinon on pituitary-gonadal axis and histological alteration of seminferous tubules in adult rat testis
Rahimi S 1, Zamiri MJ2 , Shariati M3 , Changizi-Ashtiyani S4 , Moghadamnia D5 , Rahimi A6
1- M.Sc in Animal Physiology, Department of Biology, Islamic Azad University, Kazeroon Branch, Kazeroon, Iran , somaye_biology@yahoo.com
2- M.Sc in Animal Physiology, Department of Biology, Islamic Azad University, Kazeroon Branch, Kazeroon, Iran
3- Associate Professor, Department of Biology, Islamic Azad University, Kazeroon Branch, Kazeroon, Iran
4- Associate Professor, Department of Physiology, Arak University of Medical Sciences, Arak, Iran
5- Ph.D Candidate in Animal Physiology, Islamic Azad University, Shiraz Branch, Shiraz, Iran
6- Doctor in Veterinary Medicine, Islamic Azad University, Kazeroon Branch, Kazeroon, Iran
Abstract:   (8988 Views)

Background and Objective: Diazinon is an organophosphate insecticide. , which inhibits the enzyme acetylcholinesterase. This study was done to evaluate the effect of Diazinon on pituitary-gonadal axis and histological alteration of seminferous tubules in adult rat testis. Methods: In this experimental study, 40 adult male Wistar rats were randomly allocated into five groups including control, sham and experimental 1, 2 and 3. Animals in experimental group 1, 2 and 3 were received 50, 100 and 150 mg/kg/bw of diazinon for 28 days, orally, respectively. Animals in control group did not receive any substance. Animals in sham group were received an equivalent amount of normal saline. The animals were euthanized after 28 days and a blood sample was collected via heart puncture and testes were removed for histological studies. Results: Diazinon significantly reduced serum testosterone concentration, sertoli cell, leydig cell count, primary spermatocyte and spermatid (P<0.05). Diazinon had no significant effect on the body and testis weight in the experimental groups compared to controls. Conclusion: Diazinon reduces the concentration of testosterone and cells in seminferous tubule in adult rat.
Keywords: Diazinon, Testis tissue, Testosterone, Rat
Full-Text [PDF 237 kb] [English Abstract]   (13637 Downloads) |   |   Abstract (HTML)  (199 Views)  
Type of Study: Original Articles | Subject: Physiology - Pharmacology
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Rahimi S, Zamiri MJ, Shariati M, Changizi-Ashtiyani S, Moghadamnia D, Rahimi A. Effect of Diazinon on pituitary-gonadal axis and histological alteration of seminferous tubules in adult rat testis. J Gorgan Univ Med Sci 2016; 18 (1) :23-29
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Volume 18, Issue 1 (3-2016) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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