|
Effect of Resveratrol on Sodium Valproate-Induced Oxidative Stress in the Hippocampal Tissue of BALB/c Mouse Fetuses
|
Zakieh Solbi1    , Gholamhassan Vaezi *2 , Abbasali Dehpour Juibari3 , Nahid Masoudian4 , Vida Hojati5 |
1- Ph.D Candidate in Biology, Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
2- Professor, Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran. , vaezi@yahoo.com
3- Assistant Professor, Department of Biology, Ghaemshahr Branch, Islamic Azad University, Ghaemshahr, Iran.
4- Assistant Professor, Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
5- Associate Professor, Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran. |
|
|
Abstract: (958 Views) |
Background and Objective: Sodium valproate (SV) is a commonly used antiepileptic drug; however, its therapeutic application is limited due to its potential to induce oxidative stress. Resveratrol, a natural polyphenol, possesses antioxidant properties. This study was conducted to determine the effect of resveratrol on SV-induced oxidative stress in the hippocampal tissue of BALB/c mouse fetal brains.
Methods: In this experimental study, 40 pregnant female BALB/c mice were randomly assigned to 5 groups of 8, including control, SV at 40 mg/kg/bw, SV at 40 mg/kg/bw + resveratrol at 0.6 mg/kg/bw, SV at 40 mg/kg/bw + resveratrol at 0.35 mg/kg bw, and SV at 40 mg/kg/bw + resveratrol at 0.225 mg/kg/bw. SV was administered orally per day, and resveratrol was administered daily via intraperitoneal injection. From gestational day 8 to 18, pharmacological interventions were initiated and continued until the birth of the neonates. On gestational day 18, the maternal mice were anesthetized, and 8 fetuses from each group were separated. Following the anesthesia of the fetuses, the brain tissue was dissected. Subsequently, oxidative stress parameters, including the malondialdehyde (MDA) level in nmol/mg as an index of lipid peroxidation, glutathione (GSH) level alterations in µg/mg, and protein carbonyl (PC) level alterations in nmol/mg, were evaluated in the hippocampal tissue.
Results: SV induced oxidative stress by increasing MDA (4.8 nmol/mg) and PC (51.4 nmol/mg protein), and also decreasing GSH (31.86 μg/mg) in the brain tissue compared to the control group (P<0.05). In a concentration-dependent manner, resveratrol reduced oxidative stress by decreasing MDA and PC to 3.02 and 37.21 nmol/mg, respectively, and also by increasing GSH to 49.76 μg/mg in the brain tissue. The most significant effect was observed at a concentration of 0.6 mg/kg/bw, which was statistically significant compared to the SV group (P<0.05).
Conclusion: The combined administration of SV and resveratrol culminates in a reduction in inflammation and oxidative stress-related factors in mouse fetuses.
|
|
Keywords: Epilepsy [MeSH], Resveratrol [MeSH], Oxidative Stress [MeSH], Valproic Acid [MeSH]
Article ID: Vol27-02 |
|
|
Full-Text [PDF 1015 kb]
(1007 Downloads)
| | Abstract (HTML) (60 Views)
|
|
Type of Study: Original Articles |
Subject:
Physiology - Pharmacology
|
|
|
|
|
|
|
| References |
1. Löscher W. Animal models of epilepsy for the development of antiepileptogenic and disease-modifying drugs. A comparison of the pharmacology of kindling and post-status epilepticus models of temporal lobe epilepsy. Epilepsy Res. 2002 Jun;50(1-2):105-23. doi: 10.1016/s0920-1211(02)00073-6. [ DOI] [ PubMed] 2. Wyckhuys T, Raedt R, Vonck K, Wadman W, Boon P. Comparison of hippocampal Deep Brain Stimulation with high (130Hz) and low frequency (5Hz) on afterdischarges in kindled rats. Epilepsy Res. 2010 Feb;88(2-3):239-46. doi: 10.1016/j.eplepsyres.2009.11.014. [ DOI] [ PubMed] 3. Bausch SB. Axonal sprouting of GABAergic interneurons in temporal lobe epilepsy. Epilepsy Behav. 2005 Nov;7(3):390-400. doi: 10.1016/j.yebeh.2005.07.019. [ DOI] [ PubMed] 4. Sander JW. The use of antiepileptic drugs--principles and practice. Epilepsia. 2004;45(Suppl 6):28-34. doi: 10.1111/j.0013-9580.2004.455005.x. [ DOI] [ PubMed] 5. Margulis AV, Hernandez-Diaz S, McElrath T, Rothman KJ, Plana E, Almqvist C, et al. Relation of in-utero exposure to antiepileptic drugs to pregnancy duration and size at birth. PLoS One. 2019 Aug;14(8):e0214180. doi: 10.1371/journal.pone.0214180. [ DOI] [ PubMed] 6. Battino D, Granata T, Binelli S, Caccamo ML, Canevini MP, Canger R, et al. Intrauterine growth in the offspring of epileptic mothers. Acta Neurol Scand. 1992 Dec;86(6):555-57. doi: 10.1111/j.1600-0404.1992.tb05485.x. [ DOI] [ PubMed] 7. Katzung BG. Basic and Clinical Pharmacology. 9th ed. New York: McGraw-Hill Publishing Co. 2004. 8. Chung SS, Wang NC, Treiman DM. Comparative Efficacy and Safety of Antiepileptic Drugs for the Treatment of Status Epilepticus. J Pharm Pract. 2007;20(2): 137-46. doi: 10.1177/0897190007305134. [ Link] [ DOI] 9. Li D, Bai X, Jiang Y, Cheng Y. Butyrate alleviates PTZ-induced mitochondrial dysfunction, oxidative stress and neuron apoptosis in mice via Keap1/Nrf2/HO-1 pathway. Brain Res Bull. 2021 Mar;168:25-35. doi: 10.1016/j.brainresbull.2020.12.009. [ DOI] [ PubMed] 10. Sies H. Oxidative Stress: Concept and Some Practical Aspects. Antioxidants (Basel). 2020 Sep;9(9):852. doi: 10.3390/antiox9090852. [ DOI] [ PubMed] 11. Zaric BL, Macvanin MT, Isenovic ER. Free radicals: Relationship to Human Diseases and Potential Therapeutic applications. Int J Biochem Cell Biol. 2023 Jan;154:106346. doi: 10.1016/j.biocel.2022.106346. [ DOI] [ PubMed] 12. Adewole KE, Attah AF, Osawe SO. Exploring phytotherapeutic approach in the management of valproic acid-induced toxicity. Adv Tradit Med (ADTM). 2023; 23:347-67. doi: 10.1007/s13596-021-00575-6. [ Link] [ DOI] 13. Kiskova T, Kubatka P, Büsselberg D, Kassayova M. The Plant-Derived Compound Resveratrol in Brain Cancer: A Review. Biomolecules. 2020 Jan 19;10(1):161. doi: 10.3390/biom10010161. [ DOI] [ PubMed] 14. Wang H, Dong X, Liu Z, Zhu S, Liu H, Fan W, et al. Resveratrol Suppresses Rotenone-induced Neurotoxicity Through Activation of SIRT1/Akt1 Signaling Pathway. Anat Rec (Hoboken). 2018 Jun;301(6):1115-25. doi: 10.1002/ar.23781. [ DOI] [ PubMed] 15. Zhang Y, Li Y, Wang Y, Wang G, Mao L, Zhang D, et al. Effects of resveratrol on learning and memory in rats with vascular dementia. Mol Med Rep. 2019 Nov;20(5):4587-93. doi: 10.3892/mmr.2019.10723. [ DOI] [ PubMed] 16. Faggi L, Pignataro G, Parrella E, Porrini V, Vinciguerra A, Cepparulo P, et al. Synergistic Association of Valproate and Resveratrol Reduces Brain Injury in Ischemic Stroke. Int J Mol Sci. 2018 Jan;19(1):172. doi: 10.3390/ijms19010172. [ DOI] [ PubMed] 17. Bakshi V, Sunand K, Begum N, Kakalij RM, Tekula MR. Neuroprotective Effect of Resveratrol on Valproic Acid Induced Oxidative Stress Autism in Swiss Albino Mice. International Journal of Pharmaceutical Sciences and Drug Research (IJPSDR). 2018;10:103-10. doi: 10.25004/ijpsdr.2018.100301. [ Link] [ DOI] 18. Sun XY, Qin HJ, Zhang Z, Xu Y, Yang XC, Zhao DM, et al. Valproate attenuates diabetic nephropathy through inhibition of endoplasmic reticulum stress induced apoptosis. Mol Med Rep. 2016 Jan;13(1):661-68. doi: 10.3892/mmr.2015.4580. [ DOI] [ PubMed] 19. Chang CC, Chang CY, Wu YT, Huang JP, Yen TH, Hung LM. Resveratrol retards progression of diabetic nephropathy through modulations of oxidative stress, proinflammatory cytokines, and AMP-activated protein kinase. J Biomed Sci. 2011 Jun 23;18(1):47. doi: 10.1186/1423-0127-18-47. [ DOI] [ PubMed] 20. Moreno DG, Utagawa EC, Arva NC, Schafernak KT, Mufson EJ, Perez SE. Postnatal Cytoarchitecture and Neurochemical Hippocampal Dysfunction in Down Syndrome. J Clin Med. 2021 Jul;10(15):3414. doi: 10.3390/jcm10153414. [ DOI] [ PubMed] 21. Ellman GL, Courtney KD, Andres V Jr, Feather-Stone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 1961 Jul;7:88-95. doi: 10.1016/0006-2952(61)90145-9. [ DOI] [ PubMed] 22. Ohkawa H, Ohishi N, Yagi K. Reaction of linoleic acid hydroperoxide with thiobarbituric acid. J Lipid Res. 1978 Nov;19(8):1053-57. [ PubMed] 23. Hansen JM, Lucas SM, Ramos CD, Green EJ, Nuttall DJ, Clark DS, et al. Valproic acid promotes SOD2 acetylation: a potential mechanism of valproic acid-induced oxidative stress in developing systems. Free Radic Res. 2021 Dec;55(11-12):1130-44. doi: 10.1080/10715762.2021.2017913. [ DOI] [ PubMed] 24. Koushki M, Amiri-Dashatan N, Ahmadi N, Abbaszadeh HA, Rezaei-Tavirani M. Resveratrol: A miraculous natural compound for diseases treatment. Food Sci Nutr. 2018 Oct;6(8):2473-90. doi: 10.1002/fsn3.855. [ DOI] [ PubMed] 25. Verrotti A, Scardapane A, Franzoni E, Manco R, Chiarelli F. Increased oxidative stress in epileptic children treated with valproic acid. Epilepsy Res. 2008 Feb;78(2-3):171-77. doi: 10.1016/j.eplepsyres.2007.11.005. [ DOI] [ PubMed] 26. Tanvir EM, Afroz R, Chowdhury MAZ, Khalil MI, Hossain MS, Rahman MA, et al. Honey has a protective effect against chlorpyrifos-induced toxicity on lipid peroxidation, diagnostic markers and hepatic histoarchitecture. Eur J Integr Med. 2015;7(5):525-33. doi: 10.1016/j.eujim.2015.04.004. [ Link] [ DOI] 27. Omidipour R, Zarei L, Boroujeni MB, Rajabzadeh A. Protective Effect of Thyme Honey against Valproic Acid Hepatotoxicity in Wistar Rats. Biomed Res Int. 2021 Feb;2021:8839898. doi: 10.1155/2021/8839898. [ DOI] [ PubMed] 28. Amrani A, Boubekri N, Benaissa O, Benayache F, Benayache S, Zama D. Sodium Valproate Affect Brain Antioxidant/Oxidant Status in Mice: Ameliorative Effect of Vitamin E and Chrysanthemum fontanesii Extract. Curr Bioact Compd. 2020;16(5): 576-80. doi: 10.2174/1573407215666190308152505. [ Link] [ DOI] |
|
| Send email to the article author |
|
|
|
Solbi Z, Vaezi G, Dehpour Juibari A, Masoudian N, Hojati V. Effect of Resveratrol on Sodium Valproate-Induced Oxidative Stress in the Hippocampal Tissue of BALB/c Mouse Fetuses. J Gorgan Univ Med Sci 2025; 27 (1) :9-16 URL: http://goums.ac.ir/journal/article-1-4457-en.html
|
