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Expression of Let-7d MicroRNA in Colorectal Cancer Patients
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Mohammad Arefi1    , Ayyoob Khosravi2 , Abbas Abdollahi3 , Seyed Hamid Aghaei Bakhtiari4 , Naeme Javid5 , Anvarsadat Kianmehr *6 |
1- M.Sc in Medical Biotechnology, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran.
2- Assistant Professor, Stem Cell Research Center, Department of Molecular Medicine, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran.
3- Associate Professor, Minimally Invasive Surgery & Endoscopic Research Center, Department of General Surgery, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
4- Associate Professor, Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
5- Ph.D Candidate in Molecular Medicine, Department of Molecular Medicine, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran.
6- Assistant Professor, Medical Cellular and Molecular Research Center, Department of Medical Biotechnology, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran. , kiabiotpro@yahoo.com |
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Abstract: (2985 Views) |
Background and Objective: Micro-ribonucleic acids (microRNAs) have introduced a new field in the molecular diagnosis of cancer. However, the role of circulating microRNAs in the plasma/serum of colorectal cancer patients is still unclear. This study was conducted to determine the expression of let-7d microRNA in patients with colorectal cancer.
Methods: This case-control study was conducted on 40 patients with colorectal cancer and 40 healthy people. In this study, 7 mL blood samples were collected from patients with colorectal cancer (both before and after tumor resection) and healthy individuals (only once). The serum samples were isolated and stored at - 80°C until molecular analysis. MicroRNAs were extracted from serum samples, and cDNA was synthesized. Let-7d expression was examined using the RT-qPCR method. Data were analyzed using GraphPad Prism v. 9 software. The receiver operating characteristic (ROC) curve analysis, sensitivity, and specificity were also calculated for the let-7d microRNA data to introduce a diagnostic biomarker between the preoperative patient group and the control group.
Results: In the preoperative samples of the patients, the expression of let-7d microRNA was significantly lower than that of the control group (P˂0.05). The expression of let-7d microRNA significantly increased after tumor resection compared to before. The ROC analysis for let-7d microRNA in the preoperative patient group with the control group showed that the sensitivity was 33.3%, specificity was 92.3%, and the area under the curve (AUC) was 0.622.
Conclusion: Let-7d microRNA could potentially serve as a new noninvasive diagnostic biomarker for the early detection of colorectal cancer. However, further studies are required on this subject. |
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Keywords: Colorectal Neoplasms [MeSH], let-7d microRNA [MeSH], Biomarker [MeSH]
Article ID: Vol25-31 |
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Full-Text [PDF 766 kb]
(9160 Downloads)
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Type of Study: Original Articles |
Subject:
Genetic
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