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:: Volume 18, Issue 3 (10-2016) ::
J Gorgan Univ Med Sci 2016, 18(3): 14-20 Back to browse issues page
Effect of interaventricular injection of Vitamin C on the histological structural of dentate gyrus of hipocampus in epileptic rats
R Golmohammadi * 1, B Kmalimansh2
1- Associate Professor, Department of Anatomy, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzvar, Iran. , rahimgolmohammadi@yahoo.com
2- Miswifer, Sabzevar University of Medical Sciences, Sabzvar, Iran.
Abstract:   (11765 Views)

Background and Objective: Hippocampus is the main region in cortex of the brain that involved in epilepsy. This study was done to determine the effect of intraventricular injection of vitamin C on histological structure of dentate gyrus of hipocampus in adult male epileptic rats.

Methods: In this experimental study, 40 adult male rats were randomly allocated into 5 groups (n=8). Animals in three groups were received vitamin C at dose (12.5, 25 and 50 mg/kg/bw) during 28 days, intraventricularly after were kindled by (pentylentetrazol; 40 mg/kg). Animals in forth group were received normal saline after were kindled by (pentylentetrazol; 40 mg/kg). Animals in the fifth group were received normal saline. After 28 days, rats were anesthetized by ketamin, then structure of hypocamp dissected. Histological passage was done in samples and coronal section was carried out. The sections of samples were stained by Hematoxyline-eosin. Forty fields systematicly were counted the normal neurons in dentate gyrus. Morphological change was determined by immunohistochemical method.

Results: The mean  number of normal neurons in dentate gyrus in epileptic rats which  received 25 g/kg vitamin C was more than animals in groups which were received doses of 12.5, 25 and 50 mg/kg vitamin C (P<0.05). This mean number of normal neurons in dentate gyrus of hypocamp in epileptic rats which received normal saline was lower than control and other experimental groups (P<0.05). Extensive morphological change in neurons of dentate gyrus in epileptic rats which received normal saline were observed (P<0.05). The lowest  morphological change were observed in neurons of dentate gyrus in epileptic rats which received at dose 25 mg/kg vitamin C in compared to the other groups (P<0.05).

Conclusion: Intraventricular injection of vitamin C in epileptic rat's dose dependly had neuroprotective effect on dentate gyrus neurons.
Keywords: Hypocampus, Dentate gyrus, Neuron, Epilepsy, Pentylentetrazol, Vitamin C, Rat
Full-Text [PDF 273 kb] [English Abstract]   (13875 Downloads) |   |   Abstract (HTML)  (995 Views)  
Type of Study: Original Articles | Subject: Neurosciences
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Golmohammadi R, Kmalimansh B. Effect of interaventricular injection of Vitamin C on the histological structural of dentate gyrus of hipocampus in epileptic rats. J Gorgan Univ Med Sci 2016; 18 (3) :14-20
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Volume 18, Issue 3 (10-2016) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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