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Application of PCR method in specific identification of Fusarium solani in HIV positive patients
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Ferdousi A *1   , Shahhosseiny MH2 , Bayat M3 , Hashemi SJ4 , Ghahri M5 |
1- Ph.D Candidate in Mycology, Department of Pathobiology, Islamic Azad University,, Science and Research Brach, Tehran, Iran , atousaferdousiir@yahoo.com
2- Associate Professor, Department of Microbiology, Islamic Azad University, Shahre Qods Brach, Shahre Qods, Iran and Iranian Gene Fanavar (IGF) Institute, Ashrafi Esfehani, Tehran, Iran
3- Associate Professor, Department of Pathobiology, Islamic Azad University, Science and Research Brach, Tehran, Iran
4- Professor, Department of Parasitology and Mycology, School of Public Health and Institue of Public Heath Researches, Tehran University of Medical Sciences, Tehran, Iran
5- Assistant Professor, Department of Biology, Faculty of Science, Imam Hossein University, Tehran, Iran |
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Abstract: (17429 Views) |
| Background and Objective: Fusarium solani is the common etiological agent of fungemia and disseminated fusariosis, which is associated with high incidence of mortality in immune-compromised host. Due to high level of resistance of antifungals in Fusarium solani, rapid and specific identification of organism is essential. This study was done to evaluate the PCR method for rapid and specific diagnosis of Fusarium solani in serum samples of HIV positive patients. Methods: In this descriptive study, the PCR test based on mitochondrial cytochrome b gene as the target gene with 330 bp product was optimized. PCR was applied on 45 serum samples of HIV positive patients after evaluation of sensitivity and specificity of the test. Results: In the optimized PCR test, the 330 bp product was amplified. The sensitivity of the test was a copy of Fusarium solani genome, and its specificity was 100%. Among 45 serum samples, 9 cases (20%) were positive for Fusarium solani. Conclusion: The PCR method has functional capabilities for direct, rapid and specific clinical diagnosis of Fusarium solani in HIV positive patients. |
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| Keywords: Fusarium solani, Mitochondrial cytochrome b gene, HIV, PCR |
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Full-Text [PDF 579 kb]
[English Abstract]
(21817 Downloads)
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Type of Study: Original Articles |
Subject:
Medical Mycology
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