Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1395
12
1
gregorian
2017
3
1
11
2
online
1
fulltext
fa
Determination of Ivermectin Residues in Bovine Liver Samples By ELISA and HPLC with Fluorescence Detection
تحقيقي
Original Paper
<div style="border-top: none; border-right: none; border-bottom: none; border-image: initial; border-left: 1pt dashed windowtext; padding: 0cm 0cm 0cm 4pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
<p class="Style6" style="text-align: justify; line-height: normal; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; border: none; padding: 0cm;"><span class="FontStyle42"><b><span bodoni="" mt="" style="font-size: 11pt; font-family: ">ABSTRACT</span></b></span></p>
<p class="Style5" style="text-align: justify; line-height: normal; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; border: none; padding: 0cm;"><span class="FontStyle42"><b><span bodoni="" mt="" style="font-size: 11pt; font-family: "> Background and Objectives:</span></b></span><span class="FontStyle37"><span bodoni="" mt="" style="font-size:
11.0pt;font-family:"> Ivermectin is an anti-parasitic medication frequently used in many food-producing animals. This study aimed to investigate the level of ivermectin residue in liver samples collected from slaughterhouses in Qazvin Province, Iran. </span></span></p>
<p class="Style5" style="text-align: justify; line-height: normal; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; border: none; padding: 0cm;"><span class="FontStyle42"><b><span bodoni="" mt="" style="font-size: 11pt; font-family: "> Methods:</span></b></span><span class="FontStyle37"><span bodoni="" mt="" style="font-size:11.0pt;font-family:"> Overall, 88 bovine liver samples were randomly collected and analyzed for detection of ivermectin residues. The samples were analyzed for ivermectin contamination by enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC). The samples were extracted using </span></span><span bodoni="" mt="" style="font-size:11.0pt;font-family:">liquid-liquid extraction procedure <span class="FontStyle37">for the ELISA. Solid phase extraction using a C18 column followed by fluorescence-derivatized with 1-methylimidazole and trifluoroacetic anhydride in acetonitrile were used for the HPLC assay. Recovery values obtained from the HPLC method ranged from 81.3 to 92.5%, with a relative standard deviation of 6.7-12.2%. </span></span></p>
<p class="Style5" style="text-align: justify; line-height: normal; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; border: none; padding: 0cm;"><span class="FontStyle42"><b><span bodoni="" mt="" style="font-size: 11pt; font-family: "> Results:</span></b></span><span class="FontStyle37"><span bodoni="" mt="" style="font-size:11.0pt;font-family:"> First, all samples were screened by the ELISA method. Based on the results, 16 samples (18.2%) contained no detectable levels of Ivermectin residue, while Ivermectin was found in 72 samples (81.8%). In addition, 22 of the positive samples (30.55%) contained high Ivermectin level (>50 ppb). Analysis of the samples by the HPLC method showed that eight samples (9.09%) contained ivermectin levels above the maximum residue limit.</span></span></p>
<p class="Style5" style="text-align: justify; line-height: normal; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; border: none; padding: 0cm;"><span class="FontStyle37"><span bodoni="" mt="" style="font-size:11.0pt;font-family:"> Conclusion: This study demonstrates the presence of different levels of Ivermectin residue in bovine liver samples collected from the Qazvin Province in Iran.</span></span><span bodoni="" mt="" style="font-size:11.0pt;font-family:"> Therefore, <span class="FontStyle37">regulatory authorities should ensure proper withdrawal period before slaughter of the animals.</span></span></p>
<p class="Style6" style="text-align: justify; line-height: normal; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; border: none; padding: 0cm;"><span class="FontStyle32"><span bodoni="" mt="" style="font-size:11.0pt;font-family:"> Keywords:</span></span><span class="FontStyle32"><span bodoni="" mt="" style="font-size: 11pt; font-family: "> </span></span><span class="FontStyle42"><span bodoni="" mt="" style="font-size: 11pt; font-family: ">Ivermectin, Cattle, Liver, ELISA, HPLC.</span></span></p>
</div>
Ivermectin, Cattle, Liver, ELISA, HPLC.
16
20
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-454&slc_lang=fa&sid=1
Davoodi
Jabber
E-mail:Norian.reza@yahoo.com
100319475328460012518
100319475328460012518
No
Department Of Pathobiology, Agriculture Faculty, Islamic Azad University Abhar Branch, Abhar, Iran
Reza
Norian
100319475328460012519
100319475328460012519
Yes
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Mohammad
Jalilvand
100319475328460012520
100319475328460012520
No
Department of Pathobiology, Agriculture Faculty, Islamic Azad University Abhar Branch, Abhar, Iran