Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1395
12
1
gregorian
2017
3
1
11
2
online
1
fulltext
fa
Detection of TEM, SHV and CTX-M Antibiotic Resistance Genes in Escherichia coli Isolates from Infected Wounds
تحقيقي
Original Paper
<div><strong>ABSTRACT</strong><br>
<strong> Background and Objective: </strong><em>Escherichia coli</em> is one of the most common causes of hospital-acquired infections. Extended-spectrum β-lactamase (ESBL)-producing <em>E. coli</em> strains are resistant to third-generation cephalosporins. The three main genes involved in ESBL production are <em>TEM</em>, <em>SHV</em> and <em>CTX-M</em>. Detection of ESBL-producing <em>E. coli </em>is of importance for infection control, reduction of excessive antibiotic use and epidemiological surveillance. This study aimed to detect ESBL-producing <em>E. coli </em>strains isolated from wound infections using phenotypic and molecular methods.<br>
<strong> Methods: </strong>During 2013- early 2015, 86 strains were collected from three hospitals in Isfahan, Iran. Antibiotic susceptibility testing was done using ceftazidime and ceftazidime + clavulanic acid discs. Polymerase chain reaction was used for the detection of the three resistance genes.<br>
<strong> Results: </strong>The resistance genes <em>SHV, CTX-M</em> and <em>TEM</em> were detected in 49 isolates (56.9%). In addition, 39 isolates (45%) were ESBL-producing strains. According to the results, 5 (5.8%), 14 (16.2%), 19 (22%) and 11 (12.7%) isolates contained the <em>SHV, CTX-M, TEM</em> and <em>CTX-M</em> + <em>TEM</em> genes, respectively. The frequency of <em>CTX</em> and <em>TEM</em> were significantly higher than that of <em>SHV</em> gene (P <0.05). Most of the isolated bacteria were resistant to cefazolin and sensitive to nitrofurantoin.<br>
<strong> Conclusions: </strong>There is a difference between the frequency of ESBL-positive isolates reported in the phenotypic and genotypic methods, which could be due to the lower sensitivity of the phenotypic method and impact of environmental factors on the emergence of antibiotic resistance.<br>
<strong> Keywords: </strong>Antibiotic resistance genes, ESBL, <em>TEM</em>, <em>SHV</em>, <em>CTX-M</em>, <em>Escherichia coli</em>.</div>
Antibiotic resistance genes, ESBL, TEM, SHV, CTX-M, Escherichia coli.
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35
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-452&slc_lang=fa&sid=1
Majid
Komijani
100319475328460012573
100319475328460012573
No
Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran
Majid
Bouzari
bouzari@sci.ui.ac.ir
100319475328460012574
100319475328460012574
Yes
Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran
Fateh
Rahimi
100319475328460012575
100319475328460012575
No
Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran