Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1394
12
1
gregorian
2016
3
1
10
2
online
1
fulltext
fa
Identification of Species Causing Cutaneous Leishmaniasis by PCR in Chahbahar, Iran
تحقيقي
Original Paper
<div>
<p><strong>ABSTRACT</strong></p>
<p><strong> Background and Objective:</strong> Chabahar is in Southern Iran located near the Iran-Pakistan border. Since leishmaniasis is an emerging disease in this region, this study aimed to diagnose the disease and identify different species of <em>Leishmania</em> parasite in the patients referred to the central laboratory.</p>
<p><strong> Methods:</strong> This descriptive cross-sectional study was conducted in 2011-2012 on patients referred to the central laboratory in the city of Chabahar. The sampling of lesions, slide preparation, culture and PCR specific for kinetoplast DNA (kDNA), extracted from the media and slides, were performed. The data collected by a questionnaire were analyzed by the SPSS software.</p>
<p><strong> Results:</strong> The resulted bands from the 48 tested cutaneous leishmaniasis isolates were compared with the standard strains of <em>Leishmania tropica</em>, <em>L. infantum</em> and <em>L. major</em>. All 48 investigated bands were in the 620bp region, which is related to <em>L. major</em>.</p>
<p><strong> Conclusion:</strong> Since PCR has high sensitivity and specificity, it is recommended to use kDNA (present in a unique organelle called kinetoplast) for the routine diagnosis and treatment of the disease.</p>
<p></p>
</div>
Leishmaniasis, Cutaneous, Polymerase Chain Reaction, Iran.
46
51
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-396&slc_lang=fa&sid=1
Hosein
Soleimanpoor
100319475328460010826
100319475328460010826
No
Zabol University of Medical Sciences, Zabol, Iran
Mansour
Dabirzadeh
mdabirzadeh20002000@yahool.com
100319475328460010827
100319475328460010827
Yes
Faculty of Medicine, Zabol University of Medical Sciences, Zabol, Iran
Bahman
Fooladi
100319475328460010828
100319475328460010828
No
Zabol University of Medical Sciences, Zabol, Iran