Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1394
12
1
gregorian
2016
3
1
10
2
online
1
fulltext
fa
Comparison of Disc Diffusion, Broth Microdilution and Modified Hodge Test Susceptibility Testing Of Escherichia coli Isolates to Beta-Lactam Antibiotics
تحقيقي
Original Paper
<div>
<p><strong><u>ABSTRACT</u></strong></p>
<p><u><strong> Background and Objective</strong>: One of the main tasks of clinical microbiology laboratories is to determine antibiotic resistance profiles in common pathogens and ensure the selection of effective antibiotics for certain infections.</u> <u>The aim of this study was to compare the methods of disk diffusion, broth microdilution and modified Hodge test in <em>Escherichia coli</em> isolates from urinary tract infection and diarrhea for susceptibility testing against beta-lactam antibiotics in Kerman, Iran.</u></p>
<p><strong> Methods: </strong>In this study, 432 <em>E. coli</em> isolates were collected from diarrhea (216 isolates) and urinary tract infection samples (216 isolates). The antibiotic susceptibility testing methods of disk diffusion, broth microdilution and modified Hodge test were performed according to the Clinical and Laboratory Standards Institute guidelines.</p>
<p><strong> Results</strong>: The findings of disk diffusion method showed that resistance to cefotaxime, ceftazidime, aztreonam, cefepime and imipenem was 51.15%, 30.55%, 24.30%, 15.27% and 1.85%, respectively. In the disk diffusion test, 51.15% of the isolates were resistant to at least one antibiotic, all of which were later evaluated by the broth microdilution method. Moreover, 52.94%, 17.19%, 13.12% and 0.90% of the isolates were resistant to cefotaxime, ceftazidime, cefepime and imipenem, respectively. All of the isolates were evaluated for the production of carbapenemase enzyme by the modified Hodge test and none of the isolates were found as positive.</p>
<p><strong> Conclusion</strong>: This study shows that performing carbapenem tests is very challenging, and laboratories are recommended to use secondary and independent antibiotic susceptibility tests such as modified Hodge test to confirm the carbapenem-resistant results.</p>
<p></p>
</div>
Escherichia coli, Disk Diffusion Antimicrobial Tests, Beta-Lactams.
19
24
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-390&slc_lang=fa&sid=1
Hesam
Alizade
yahoo.com
100319475328460010914
100319475328460010914
Yes
Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran
Fatemeh
Fallah
100319475328460010915
100319475328460010915
No
Faculty of Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran
Reza
Ghanbarpour
100319475328460010916
100319475328460010916
No
Faculty of Veterinary Medicine, Shahid Bahonar University, Kerman, Iran
Hosein
Goudarzi
100319475328460010917
100319475328460010917
No
Faculty of Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran
Hamid
Sharifi
100319475328460010918
100319475328460010918
No
Institute of Future Studies in Health, Kerman University of Medical Sciences, Kerman, Iran
Mohammad Reza
Aflatoonian
100319475328460010919
100319475328460010919
No
Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran