Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1394
10
1
gregorian
2016
1
1
10
1
online
1
fulltext
fa
Mutations at Nucleotide 1762, 1764 and 1766 of Hepatitis B Virus X Gene in Patients with Chronic Hepatitis B and Hepatitis B-Related Cirrhosis
تحقيقي
Original Paper
<div>
<p><strong>Abstract</strong></p>
<p><strong> Background and objective</strong>: Hepatitis B virus (HBV) is a DNA virus with high tendency toward hepatic tissue. There are currently about 3 million HBV-infected people and 350 to 400 million chronic carriers of this virus in the world. X protein plays a role in the over-expression of oncogenes, carcinogenicity of liver cells and overlaps with the basal core promoter of the virus. Mutations at specific nucleotides of this region increase viral replication and liver disease progression. The aim of this study was to investigate the frequency of mutations at nucleotides 1762, 1764 and 1766 of HBV X gene in patients with chronic hepatitis B and hepatitis B-related cirrhosis.</p>
<p><strong> Methods</strong>: In this study, 102 patients including 68 chronic hepatitis patients and 34 patients with hepatitis B-related cirrhosis were enrolled. After DNA extraction, HBV X gene was amplified and sequenced using Semi Nested-PCR. Obtained gene sequences were compared with the standard sequence of HBV virus X gene available in the gene bank (Okamoto AB033559). Then, the mutations in the gene X of HBV were identified.</p>
<p><strong> Results</strong>: Comparison of the standard sequence with sequences obtained from patients showed the presence of A1762T / G1764A mutation in 12 chronic (17.64%) and 13 cirrhotic (38.23%) patients. Also, C1766G / G1764T mutations were found in 8.23% of chronic patients and 17.64% of cirrhotic patients.</p>
<p><strong> Conclusion</strong>: A1762T / G1764A mutations in the overlapping region of the basal core promoter with gene X C-terminal may lead to liver disease progression from chronic hepatitis to cirrhosis, by changing the amino acid sequence of the X protein.</p>
<p><strong> </strong></p>
</div>
HBV, BCP Mutations, X Gene Mutations, Cirrhosis, Chronic
31
35
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-381&slc_lang=fa&sid=1
Farzane
Salarneia
100319475328460010858
100319475328460010858
Yes
Department of of Virology
Sare
Zhand
100319475328460010859
100319475328460010859
No
PhD Student of Microbiology
Behnaz
Khodabakhshi
100319475328460010860
100319475328460010860
No
Department of Microbiology
Alijan
Tabarraei
100319475328460010861
100319475328460010861
No
Golestan University of Medical Sciences, Gorgan, Iran
Mohammad Ali
Vakili
100319475328460010862
100319475328460010862
No
Golestan University of Medical Sciences, Gorgan, Iran
Naeme
Javid
100319475328460010863
100319475328460010863
No
Department of Microbiology
Masoud
Bazori
100319475328460010864
100319475328460010864
No
Department of Microbiology
Abdolvahab
Moradi
100319475328460010865
100319475328460010865
No
Department of Microbiology