Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1392
4
1
gregorian
2013
7
1
7
2
online
1
fulltext
fa
جداسازی، شناسایی ملکولی و تعیین اثر ضد قارچی باسیلوس های جداشده از خاک ریزوسفریک منطقه گرگان علیه تریکوفیتون منتاگروفایتس
Isolation, Molecular Identification and Evaluation of Antifungal Effects of the Bacilli Isolated from Rhizospheric Soil of Gorgan Region against Tricophyton Mentagrophytes
تحقيقي
Original Paper
Abstract Background and objective: Soil bacteria, particularly Bacillus genus have the potential of producing a range of bioactive substances with antimicrobial and antifungal properties. They have the ability to produce hundreds of active and effective biologic compound against microorganisms. Therefore, it seems to be a proper candidate in the biocontrol of fungal pathogenesis. Material & Methods: In this study, soil samples were collected from different parts of Gorgan in order to isolate Bacillus and to determine their antifungal activity against T.mentagrophytes. The Isolates that had the highest antifungal effects were analyzed by PCR and 16s rRNA sequencing. Results: of 54 strains, 14 have antifungal activity. The Isolates, S4 and S12, identified as B.cereus and B.thuringiensis respectively show the highest antidermatofit effect. These isolates based on 16s rRNA sequence analysis show 97% homology with Bacillus cereusstrain KU4 and Bacillus thuringiensisstrain ucsc27. Conclusion: According to the results, it seems that the soil Bacilli have biocontrol potential against dermatophytic agents such as T.mentagrophytes. Keywords: Antifungal effects, Bacillus, Rhizospheric soil, T.mentagrophytes
Antifungal effects, Bacillus, Rhizospheric soil, T.mentagrophytes
18
22
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-146&slc_lang=fa&sid=1
HR
Pordeli
حمیدرضا پردلی
h_pordeli@yahoo.com
100319475328460012888
100319475328460012888
Yes
Assistant Professor of Mycology, Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Gorgan Branch
دانشکده علوم پایه دانشگاه آزاد اسلامی واحد گرگان،گرگان،ایران
SJ
Hashemi Hazaveh
سید جمال هاشمی هزاوه
100319475328460012889
100319475328460012889
No
Associated Professor of Mycology, Department of Mycology, Faculty of Veterinary Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran
دانشکده علوم دامپزشکی،
M
Jamshidian
محمود جمشیدیان
100319475328460012890
100319475328460012890
No
Professor of Microbiology, Department of Microbiology, Faculty of Veterinary Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran
دانشکده علوم دامپزشکی
M
Bayat
منصور بیات
100319475328460012891
100319475328460012891
No
Associated Professor of Mycology, Department of Mycology, Faculty of Veterinary Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran
دانشکده علوم دامپزشکی