Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1392
4
1
gregorian
2013
7
1
7
2
online
1
fulltext
fa
تولید سازه ژنی نوترکیب حاوی قطعه کد کننده هپسیدین انسانی
Production of Recombinant Bacmid Containing the Coding Sequence of Human Hepcidin
تحقيقي
Original Paper
Abstract Background and objective: Hepcidin is a cystein-rich antimicrobial peptide, which is secreted by the liver. It fights against wide spectrum of bacteria, viruses and fungi and it is a major regulator of iron homeostasis. Today, scientists have made many efforts on the production of hepcidin. Baculovirus expression system is one of the best eukaryotic expression systems for production of recombinant hepcidin and production of the recombinant vector is one of the most important steps in this expression system. Material & Methods: First, the total RNA was separated from HepG2 cell line as a source of hepcidin expression. Then, after synthesis of total cDNA, human hepcidin sequence was amplified, using specific primers by PCR method. Next, hepcidin sequence was cloned into pTZ57R/T vector. After digestion of recombinant vector using ECoRI and BamHI restriction enzymes, recombinant pFastBac HT B vector containing human hepcidin cDNA was produced. Results: Coding sequence of human hepcidin is correctly cloned into pTZ57R/T vector and sub cloning into pFastBac HT B vector is performed successfully. The presence of a clear band near 274 bp resulted from PCR amplification and restriction enzyme are the confirmation of the cloning of human hepcidin. Conclusion: According to our knowledge, the present study is the first work that focuses on recombinant vector containing coding sequence of human prohepcidin. This recombinant vector can be used for human hepcidin production. Key words: Vector, Hepcidin, Iron
Bacmid, Hepcidin, Iron
1
6
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-143&slc_lang=fa&sid=1
N
Keyhanvar
ندا کیهان ور
100319475328460012885
100319475328460012885
No
MSc Student of Medical Biotechnology, Faculty of Advanced Medical Technologies
دانشکده فناوری های نوین،دانشگاه علوم پزشکی گلستان
A
Tabarraei
علیجان تبرائی
100319475328460012886
100319475328460012886
No
Assistant Professor of Virology, Department of Microbiology
Y
Yazdani
یعقوب یزدانی
yazdani@goums.ac.ir
100319475328460012887
100319475328460012887
Yes
Assistant Professor of Immunology, Laboratory Science Research Center and Infectious Diseases Research Cente