Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1398
2
1
gregorian
2019
5
1
13
3
online
1
fulltext
fa
Investigating Antibacterial Effects of Latrodectus Dahli Crude Venom on Escherichia coli, Staphylococcus aureus and Bacillus subtilis
فیزیولوژی ورزشی
Sport Physiology
تحقيقي
Original Paper
<div><strong>ABSTRACT</strong><br>
<strong> Background and Objectives:</strong> Nowadays, infections with antibiotic-resistant bacteria are among the most important causes of mortality worldwide. This has attracted the attention of researchers to seek suitable alternatives for antibiotics. The venom of many toxic species such as arthropods has antibacterial properties. In this study, we investigated antibacterial effects of crude venom of <em>Latrodectus dahli</em> on <em>Escherichia coli, Staphylococcus aureus, </em>and<em> Bacillus subtilis.</em><br>
<strong> Methods:</strong> Lyophilized crude venom of <em>L. dahli</em> was dissolved in 50 mM Tris-HCl buffer. Protein concentration was determined by the Bradford assay. Then, the bacteria were exposed to different concentrations (31.25-250 ng/mL) of the crude venom. Inhibitory activity of the venom against the bacteria was determined by MTT assay and determining minimum inhibitory concentration (MIC).<br>
<strong> Results: </strong>Results of the MTT assay showed that the crude venom significantly inhibited the growth of <em>E. coli</em> (31.25 and 62.5 ng/mL), <em>S. aureus</em> (at 250 ng/mL) and <em>B. subtilis</em> (at 125 and 250 ng/mL). In the MIC experiment, the crude venom significantly inhibited the growth of <em>E. coli</em> (at concentrations of 31.25 and 62.5ng/mL), <em>S. aureus</em> (at concentrations of 31.25-250 ng/mL) and <em>B. subtilis</em> (at concentrations of 31.25-250ng/mL).<br>
<strong> Conclusion:</strong> The crude venom of <em>L. dahli</em> and its components showed relatively strong antibacterial effects.<br>
<strong> Keywords:</strong> Spider venoms, Black Widow Spider, Antibacterial agent, Drug-resistance.</div>
Spider venoms, Black Widow Spider, Antibacterial agent, Drug-resistance.
14
19
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-558&slc_lang=fa&sid=1
Mohsen
Mousavi
100319475328460015621
100319475328460015621
No
Department of Biology, Faculty of Basic Science, Imam Hossein University, Tehran, Iran
Behrooz
Johari
100319475328460015622
100319475328460015622
No
Department of Medical Biotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
Jamil
Zargan
100319475328460015623
100319475328460015623
Yes
Department of Biology, Faculty of Basic Science, Imam Hossein University, Tehran, Iran
Ashkan
Haji Noor Mohammadi
100319475328460015624
100319475328460015624
No
Department of Biology, Faculty of Basic Science, Imam Hossein University, Tehran, Iran
Hamid Reza
Goudarzi
100319475328460015625
100319475328460015625
No
Department of Venomous Animals and Antivenin Production, Razi Vaccine and Serum Research Institute, Karaj, Iran
Saeed
Dezianian
100319475328460015626
100319475328460015626
No
Department of Biology, Faculty of Basic Science, Imam Hossein University, Tehran, Iran
Hani
Keshavarz Alikhani
100319475328460015627
100319475328460015627
No
Department of Biology, Faculty of Basic Sciences, Razi University, Kermanshah, Iran