TY - JOUR T1 - A Simplified Van Erth Single Nucleotide Polymorphism (SNP) Typing Method of Bacillus Anthracis Applicable by Traditional Thermocycler Machines TT - معرفی یک نسخه ساده از روش Van Erth در مطالعه SNP typing باسیلوس آنتراسیس سازگار با ماشین های ترموسایکلر معمولی JF - mljgoums JO - mljgoums VL - 9 IS - 1 UR - http://mlj.goums.ac.ir/article-1-612-en.html Y1 - 2015 SP - 97 EP - 103 KW - Bacillus Anthracis KW - Genotyping KW - SNPs KW - PCR N2 - Abstract SNP typing is now a well-established genotyping system in Bacillus anthracis studies. In the original standard method of Van Erth, SNPs at 13 loci of the B. anthracis genome were analyzed. In order to simplify and make appropriate this expensive method to low-budget laboratory settings, 13 primer pairs targeting the 13 corresponding SNPs were designed. Besides, a universal PCR protocol was developed to enable simultaneous amplification of all loci by conventional PCR machines. The efficiency of this approach was approved by applying on nine isolates of B. anthracis. We recommend using this modified procedure as an efficient alternative to Van Erth method until developing newer and affordable techniques. Keywords: Bacillus Anthracis, Genotyping, SNPs, PCR M3 ER -