Search published articles


Showing 2 results for Esbls
The effect of silver nanoparticles on Gram-negative bacilli Resistant to Extended-Spectrum Β-Lactamase Enzymes
Doudi M, Naghsh N, Heiedarpour A,
Volume 5, Issue 2 (10-2011)
Abstract
Abstract: Background and objectives: Antibiotic resistant to Antimicrobial agents is one of the most important concern in hospitals, which can lead to increased costs, treatment fails and mortality rates. The aim of this study was identification of Gram-negative bacilli resistant to extended-spectrum β-lactamase Enzymes (ESBLs) and determination of the effect of silver nanoparticles on them. Materials & Methods: of 276 clinical samples referred to three hospitals of Isfahan city, 186 gram negative bacilli were studied. To recognize ESBLs production, the bacilli was assessed by disk diffusion method and confirmed by DDT and Double Disk approximation Test. The ESBL producing bacteria were subjected to increasing concentrations (12.5, 25, 50,100,200,400 and 500 ppm) of silver nanoparticles, prepared in Tehran Nano Pars Company, and Inhibitory zone diameter was measured. Results: of 186 isolates, 140 (%75/3) are gram-negative bacilli producing ESBLs and 46 (24.7%) of them without this capability. Most of ESBLs bacteria are belonged to urine infections and the most prevalent bacterium is Klebsiella pneumonia. All samples are sensitive to the nano silver solution with density of 100 ppm. Enterobacter aerogenes (24 mm) and Pseudomonas aeruginosa (23mm) have the greatest Inhibitory zone diameter in the presence of 500 ppm of silver nanoparticles. Conclusion: It seems that silver nanoparticles have inhibitory effect on all studied gram negative bacilli. The inhibitory effect of silver nanoparticles against ESBL producing Gram negative bacteria is dose depended Keywords: Gram-negative bacilli, ESBLs, silver nanoparticles.
Prevalence of blaoxa-1 and blashv Genes in E. coli Isolates from Hospitalized Patients in Rasht
Mohammad Faezi Ghasemi , Seyede Negin Dibadji,
Volume 10, Issue 5 (9-2016)
Abstract

ABSTRACT

      Background and Objective: Extended-spectrum beta-lactamases (ESBL) are widely produced by Escherichia coli strains. The aim of this study was to determine frequency of blaOXA-1 and blaSHV genes in E.coli strains isolated from patients hospitalized in city of Rasht, Iran.

       Methods: In this cross-sectional study, 200 samples were collected from patients. The E. coli strains were identified using morphological characteristics and biochemical tests. Antimicrobial susceptibility testing was performed. The prevalence of the blaOXA-1 and blaSHV genes in the E. coli isolated was assessed by PCR method.

       Results: Overall, 160 E. coli strains were isolated. Of these, 83 (51.9%) showed ESBL activity while 71 (48.1%) did not. All positive strains were resistant to cephalothin. Moreover, 98.8% of ESBL-producing strains were resistance to amoxicillin, cefotaxime and ceftriaxone. The prevalence of the blaOXA-1 and blaSHV genes in ESBL-producing strains were 45% and 17%, respectively. In addition, 28.9% of the strains had both genes while the genes were absent in 9.6% of the strains.

      Conclusion: Prevalence of the blaOXA-1gene is higher than that of the blaSHV gene. The absence of both genes in some isolates indicates the possible role of other genes in the ESBL activity.

      Keywords: Prevalence, ESBLs, Escherichia coli, blaOXA-1gene, blaSHV gene.


Page 1 from 1     

© 2007 All Rights Reserved | Medical Laboratory Journal

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.