Showing 3 results for Plasmodium Falciparum
A Jamshidi, M Mousavi Ghahfarrokhi, A Gharaei, A Ebrahimzadeh, M Jaffari Modrek, A Ansari Moghadam, S Mohammadi,
Volume 8, Issue 1 (4-2014)
Abstract
Abstract
Background and Objective: The Plasmodium falciparum EBA-175, via Sialic acid dependent glycophorin A, binds to red blood cells and thus plays a critical role in cell invasion. Some part of second allele in its gene encoding in FCR-3 (Section F) and CAMP (Section C) can be found. This study aimed to determine the prevalence of Plasmodium falciparum EBA-175KD alleles in southeastern Iran.
Material and Methods: In this cross-sectional study, using polymerase chain reaction Nest (Nested-PCR) with specific primers was used for the two parts of the EBA-175 gene to be proliferated. Ninety–four microscopic positive blood samples from individuals infected by Plasmodium falciparum were obtained from four different locations in southeastern Iran.
Results: Of 94 positive samples, 88 were antigen EBA-175KD. Genotype CAMP (714 bp) and FCR-3 (to 795 bp), respectively, in 31 (32.97 %) and 49 (52.12 %) were found. Eight samples have both FCR-3 and CAMP.
Conclusion: Both of EBA-175KD dimorphic genes were found. The frequency of FCR-3 allele was higher in the South East of Iran. Thus, this pattern can be considered in making Plasmodium falciparum vaccines for this area.
Key words: Plasmodium Falciparum Erythrocyte Binding Antigen-175 South-East of Iran
Adel Ebrahimzadeh, Tahereh Davoodi , Abbas Pashaei Naghadeh ,
Volume 9, Issue 4 (10-2015)
Abstract
Abstract
Background and Objectives: Plasmodium falciparum merozoite surface protein-1 (PfMSP-1) is a promising vaccine against malaria during its blood stages which play an important role in immunity to this disease. Polymorphic nature of this gene is a major obstacle in making an effective vaccine against malaria. In this study, the genetic diversity of Plasmodium falciparum isolates was investigated in Sistan-Baluchestan Province using allelic families of the MSP-1.
Methods: From March/April 2011 to August/September 2012, 94 blood samples were collected from patients with falciparum malaria who were living in four districts of Sistan-Baluchestan Province. The extracted genomic DNA and genetic diversity of MSP-1 block 2 were evaluated by nested polymerase chain reaction.
Results: From a total of 94 patients, 89 patients (94.7%) had positive PCR results and the remaining five patients were excluded. Seven different alleles of MSP-1 were identified through size difference on agarose gel. Comprising 46.1% of the samples, MAD20 was identified as the predominant MSP-1 allelic family, while the RO33 family had the lowest frequency (with 7.9%). In 10% of samples infection with two alleles was observed.
Conclusion: The results of this study suggest that genetic diversity of PfMSP-1 in Southeastern Iran is relatively low and most infections originate from a clone that is consistent with an area of low malaria transmission. This information is useful for the prevention and control of malaria in Iran.
Keywords: Merozoite Surface Protein 1, Plasmodium Falciparum, Polymerase Chain Reaction
Sk.riyaz Parveen, G Vamshi Deepak, Amulya Boddapati,
Volume 17, Issue 2 (3-2023)
Abstract
Background and objectives: Malaria causes a wide spectrum of hematological and clinical manifestations. This study aimed to identify the alterations in the clinical and hematological parameters in patients infected with Plasmodium vivax, Plasmodium falciparum, and mixed species.
Methods: The study included 126 smear-positive malaria cases, and various hematological parameters were studied.
Results: The frequency of P. vivax, P. falciparum, and mixed species was 53.9%, 36.5%, and 9.6%, respectively. Anemia (hemoglobin <11 gm%) was seen in 79.3% of the cases, and severe anemia (hemoglobin <5g%) was detected in 27.7% of the cases. A decrease in red blood cell count was observed in 67% of P. falciparum and 47% cases of P. vivax cases. Increased red cell distribution width and erythrocyte sedimentation rate were seen in 81% and 78% of the cases, respectively. Leukocytosis and leucopenia were detected in 15% and 16% of all malaria cases, respectively. Thrombocytopenia was associated with 78% of cases infected with P. vivax. The degree of anemia was correlated with the parasite load, and the degree of parasitemia was correlated with the extent of thrombocytopenia. There were also significant variations in the mean corpuscular volume, hematocrit, mean corpuscular hemoglobin concentration, and platelet counts among malarial species (p<0.05).
Conclusion: Malaria is frequently associated with anemia, thrombocytopenia, and leukopenia. Thrombocytopenia is mostly associated with P. vivax infection. On contrary, leukopenia is more prevalent in P. falciparum, followed by P. vivax and mixed parasitemia.
