---

Abstract

Background and objectives:

most common causes of morbidity and mortality in industrial and

developing countries. Recent studies have suggested that

A coronary heart disease is one of the

Helicobacter pylori

heart disease therefore, this study was carried out in Gorgan, Iran, to

show the relationship between coronary disease and Helicobacter

pylori infection.

caused infection may be associated with chronic

Materials and Methods:

carried out on 109 patients suffering from acute coronary syndrome

and 85 healthy individuals, ELISA was used to determine Anti

Helicobacter pylori Anti bodies (IgA, IgG ).

In this cross sectional case-control study

Results:

while in control group were %32.9 and %62.4. There was significant

difference between IgA of two groups (p<0.007). Simultaneous

presence of both IgG and IgA in patients affected by Coronary disease

was meaningful (p<0.003).

IgA and IgG antibodies of case group were %51.4 and %53.2

Conclusion

be related to coronary disease, we suggest their investigation in

suspected individuals.

: Since Simultaneous presence of both IgG and IgA may

Key word

Antibody

: Acute Coronary syndrome, Helicobacter Pylori,

---

Abstract Background and objectives: Recurrent Abdominal Pain (RAP) syndrome is a common disorder, especially in children with beta thalassemia major. These patients are predisposed to heart diseases which are caused by hemochromatosis (Iron overload), resulting in sudden death. Because of the role of Helico bacter pylori in causing abdominal pain and peptic ulcer, and in increasing the risk or exacerbating of heart diseases, it can be important in beta thalassemia major. This study aimed at determining the frequency of anti- H.pylori class IgA in patients suffered from beta thalassemia major. Material and Methods: In this descriptive-analytic Study, 132 beta thalassemia major Patients and 135 healthy cases were matched by age, sex and ethnic as a control group. The sera were separated from clot in first Seroepidemiology and along with the filled questionnaires sent to Immunology-hematology Laboratory of paramedical school, in which the samples were tested by ELISA method with Diagnostic kit for anti-H.pylori IgA and IgG classes detection. We analyzed the Data by chi square and Independent T and Fisher tests. Results: the results show that the difference between the anti-H.pylori class IgA in case (22.7%) and control group (17.8%) is not significant (P=0.6), But it is significant between anti-H.pylori IgG class, 81.8% for cases and 54.8% for controls (P<0.001). Conclusion: Based on the results, the rate of anti-H.P IgA class in case is 1.131 times more than control group, which is not significant. On the other hand , the rate of anti-H.P IgG is 1.478 times more than control group which is significant. Key words: Thalassemia, Helicobacter pylori, Gorgan, Sero epidemiology.

---

Abstract Background and objectives: Helicobacter pylori is a Gram- negative bacteria causing diseases such as peptic ulcer and gastric cancer. In this research ,we determined the level of three types of antibody IgG, IgM and IgA in ordinary people of Izeh. Material and Methods: After collecting blood samples from 263 cases, the serum were obtaind and using the ELISA method the amount of IgG, IgM and IgA, against Helicobacter pylori measured. Results: Eleven samples(4.3%) out of 263 casese are positive for IgM anti-Helicobacter pylori. The frequency of IgA and IgG is 28(10.6%) and 201(76.4%),respectively. Conclusion: Based on results more than 70% of people in Izeh have Anti- Helicobacter pylori antibody in their serum, which is higher than that the other studied in Iran and also than developed countries. Key words: Seroepidemiology, Helicobacter pylori, Izeh

---

Abstract Background and objectives: Helicobacter pylori is a helical gram negative bacterium with polar flagella, discovered by Warren and Marshall in 1983. Helicobacter pylori exist in the stomach mucus tissue of less than 20% of people under 30 years old, but this amount would increase up to 40% and 60% in 60- year- old people. The aim of this study was to compare three methods of culture media, direct slide staining and the urease test for the rapid diagnosis of bacterium in case of peptic or duodenal ulcer. Material and Methods: In This descriptive study, duplicate biopsy specimens were taken from 82 clients referring to four different Hospitals .In endoscopy room of the Hospitals, a rapid urease test were carried out on one of duplicate specimens for the presence or non-presence of Helicobacter pylori. In order to see the Helicobacter pylori in the tissues, three slides using foushin, giemsa, and gram staining were prepared from the second specimens. Then, the specimens were incubated into selective culture media and incubated for 4-6 days in micoraerophilic condition. Results: Of 82 tested specimens 70(85.5%) and 66(80.5%) are identified as Helicobacter pylori by positive urease and culture medium, respectively. The frequency of foushin, giemsa, and gram staining are 67 (81.7%), 66 (80.5%), and 61 (74.4%), respectively. The foushin staining is the best with 100% sensitivity among the other methods. Conclusion: Based on difference between proportions, There is no significant difference between staining methods (foshin, giemsa, gram staining) and culture media in all cases. Key words: Helicobacter pylori, microscopic methods, urease test, culture media, identification

---

Abstract Background and objective: The effects of lactobacillus Species and their metabolites on dairy products were proved as probiotics on pathogenic bacteria. The aim of this study was to isolate the lactobacillus from dairy product (Yogurt, cheese, milk and whey …) and to determine the antimicrobial activity against some pathogenic bacteria (Salmonella typhimurium, E.coli, Helicobacter pylori. Material and methods: Samples of dairy products were collected and were serially diluted in peptone medium. To isolate lactobacillus, diluted sample were plated on MRS Agar medium and incubated at 37ºc for 48-72h in anaerobic condition. Identification of the lactobacilli was performed according to their morphological, cultural, physiological and biochemical characteristics. The inhibitory effect of isolated lactobacillus strains were performed against pathogenic bacteria by Agar Well Diffusion Assay and Disc method. Results: of 50 samples of isolated Lactobacillus from dairy products (including 11 identified Lactobacillus), 19 samples have inhibitory effect on pathogenic bacteria such as Salmonella typhimurium, Escherichia coli and Helicobacter pylori. The most identified strains areLactobacillusbulgaris, Lactobacillus Salivarius and Lactobacillus delbrueki. Conclusion: According to this study, some lactobacillus strains isolated from dairy products are useful in the management of diarrhea and other gastrointestinal diseases, and the use of these strains can be useful for prevention and treatment. Keywords:Lactobacillus, Dairyproducts, SalmonellaTyphimurium, E.coli, Helicobacter pylori

---

Abstract Background and Objective: Helicobacterpylorus is the major cause ofinflammation andulcer instomach, and immunoglobulin IgG is one of the antibodies produced against it, which is important in the course and diagnosis of the previoussufferers. The awareness of the prevalence of this disease can be helpful for the physicians to choose the way of treatment. Material and Methods: In these cross-sectional study, 516patients referred to laboratory was studied. After separating the serum, Antibody Helicobacter pylori IgGtest wasdone by ELISA method. Results: of 516, 156 (30.2 %) of the patients have a positive result, 51(32.7%) are males and 105 (67.3 %) are females. Positive percent of males (43.5%) is greater than females (26.5%).Over-45-year-old women (9.8 %) have the highest percentage of disease titers. Conclusion: The percent of positive cases in men is more than that the women. Over-45-year-old women (9.8 %) have the highest percentage of positive case. Keywords: Helicobacter pylori IgG, Tehran, ELISA

---

Abstract Undoubtedly, H. pylori is the major human gastric pathogen, which infects the mucosal epithelium in 50% of world population. However, H. pylori infection is relatively prevalent globally the majority of infected individuals are asymptomatic. The recent epidemiological studies show that the various gastro intestinal complications can be the result of genetic variation in H. pylori strains. To date, different virulence factors had been suggested for H. pylori, but none of them can be a good biomarker for specific gastric disorders. In this review article, we aim to describe a comprehensive view on what we found in H. pylori virulence research in Iran. Hopefully, in parallel with new advances in sequencing systems, we will have better overview of virulence phenomenon of H. pylori in near future. Keywords: Helicobacter pylori, Iran, virulence, homB, dupA

---

Abstract Background and Objective: Helicobacter pylori (H. Pylori) infection is related to chronic gastritis, peptic ulcer, duodenal ulcer and gastric cancer. Thus, identification and treatment of the infection have a considerable importance. The aim of this study was to compare three methods of Histopathology, Culture and Rapid Urease test (RUT) in identification of H. Pylori in gastric biopsy specimens. Material and Methods: The participants were 153 patients (64 women and 89 men) suffering from digestive complaints, who referred to the endoscopy department of Shahid Beheshi Hospital in Hamadan, Iran. Three gastric biopsy samples were collected from each patient and examined by standard RUT, Histopathology and culture methods for diagnosis of H. Pylori. Results: Out of 153 patients, 69.9%, 27.4% and 2.6 % had gastritis, gastric ulcer and gastric cancer, respectively. The rate of infection with Urease test, culture and histology were identified 49.7%, 54.2%, and 89.5%, respectively. The sensitivity and specificity of the RUT result at first hour and after the first up to 24 hours were 55.4% and 80%, and 55.4% and 66/7%, respectively. The sensitivity and specificity of culture method were 60.6% and 100%, respectively. Conclusion: Based on the results, Histopathology method has a more sensitivity than both Culture method and rapid urease test for diagnosis of H. Pylori, and RUT is more specific when done in the first hour rather than after the first hour. Key words: Helicobacter pylori, rapid urease test methods, RUT, Histopathology

---

---

 

---

Background: Intestinal infections with Helicobacter pylori mainly occur during childhood. If contracted, these infections may cause chronic gastritis, frequently leading to peptic ulcer disease in later life. This study aims to detect the prevalence of H. pylori infections in patients with active gastritis.
Methods: The study included 150 participants who were consuming daily anti-gastritis drugs to reduce the gas and were considered active gastritis patients and were recruited from Lekhnath 12, now known as Pokhara metropolitan-30, from May 2018 to March 2019. They were screened for H. pylori antibodies for detection of infection by the immunochromatographic rapid detection kit, and the data were analyzed using SPSS 2016.
Results: Serum anti-Helicobacter pylori antibodies were used to detect the presence of Helicobacter pylori in these participants. Among the 150 participants, 54 were males (36%) and 96 were females (64%). The results showed that 47 (31.3%) of the patients were positive for anti-Helicobacter pylori test. The age group 30 to 40 had the highest prevalence of 21 (14.0%). Using public water showed the highest prevalence with a P value of 0.04.
Conclusion: There should be an additional aspect required for the diagnosis and treatment of gastritis, which is the healthcare providers' and patients' awareness of the cause and most efficient treatments for this medical condition. Using only anti-gastritis drugs is not sufficient; treatment against Helicobacter pylori requires the right pathway of treatment by the use of several antibiotics.