Search published articles


Showing 4 results for azadi

Azadi F, Hedayat Mofidi, M , Ali Arab, A , Hedayat Mofidi, H , Khorasaninjad, R , Ghasemi-Kebria, F , Roshandel, Gh ,
Volume 9, Issue 3 (Jul,Aug2015[PERSIAN] 2015)
Abstract

Abstract

Background and Objective: Helicobacterpylori(HP) is the most common bacterial infection in the world and it may be associated with chronic gastritis, pepticulcer disease, and gastricadenocarcinoma. The aim of this study was to determine the prevalence of HP in student of Golestan University of Medical Sciences (GOUMS).

Material and Methods: This cross-sectional study was conducted on students ofGOUMS in 2012. Anti-HP IaG and IgA were assessed byELISA method.

Results: The participants recruited  were 287 in that 101 (35.2%) were male and the rest female. Of 287 , 239 (83.3%) were positive for HP. The proportion of  men was 91.1% and women 79% (P =0.03). There was no significant relationship between HP infection and variables such as  age, blood group , place of residence, nativity, and level of education.

Conclusion: The prevalence of H. pylori infection in the students of  Golestan University of Medical Sciences is high.

Keywords: Helicobacter Pylori, Antibodies, Students, Golestan


Ezzat Allah Ghaemi, Fahimeh Azadi, Naeme Javid, Hanieh Bagheri,
Volume 14, Issue 4 (Jul-Aug 2020)
Abstract

Background and objectives: Drug resistance in Staphylococcus aureus and Escherichia coli, as severe pathogenic bacteria, has become a health challenge. However, nanoparticles have been introduced as effective candidates for their eradication. In this study, we investigated presence of genes involved in conferring resistance to silver nanoparticles in S. aureus and E. coli isolates and evaluated its association with minimal inhibitory concentration (MIC) of the nanoparticles against these isolates.
Methods: The MIC of silver nanoparticles against 121 clinical isolates of E. coli and 183 S. aureus isolates was assessed by broth microdilution assay. Presence and expression of the silver resistance genes (silE, silR/S) in the isolates were investigated by PCR and real-time PCR, respectively.
Results: The silE gene was found in three (1.6%) S. aureus and four (3%) E. coli isolates. MIC of silver nanoparticles against S. aureus isolates with the silE gene was 1, 2 and 8 µg/ml. Moreover, the MIC of the nanoparticles against silE-positive E. coli isolates was 16 μg/ml in three cases and 8 μg/ml in one case. None of the S. aureus isolates contained the silR/S gene, but presence of both silE and silR/S was confirmed in two E. coli isolates. Real-time PCR showed no sil expression in the isolates containing the resistance genes.
Conclusion: The frequency of the silver resistance genes among S. aureus and E. coli isolates is very low. There is no relationship between presence of the resistance genes and the MIC value of silver nanoparticles.
Fahimeh Azadi, Masoomeh Rezanezhadi, Hanieh Bagheri, Laith B Alhusseini, Hamid Reza Joshaghani,
Volume 15, Issue 4 (Jul-Aug 2021)
Abstract

Background and objectives: Tuberculosis (TB) is a serious public health problem and a significant diagnostic and therapeutic challenge worldwide. Molecular diagnostic techniques are crucial parts of the World Health Organization’s new tuberculosis control strategy. This study aims to identify Mycobacterium tuberculosis and rifampin resistance in pulmonary and extra-pulmonary clinical specimens using the Gene Xpert MTB/RIF assay.
Methods: The study was carried out on 220 specimens from pulmonary and extra-pulmonary TB patients that were sent to the Kavosh Laboratory in Gorgan (Iran) during 2018-20. The Gene Xpert MTB / RIF method was applied to detect M. tuberculosis and rifampin resistance.
Results: Of 220 specimens, 15 (6.81%) were found to be positive, four (26.6%) of which were related to pulmonary and 11(73.3%) to extra-pulmonary specimens. None of the positive samples was resitant to rifampin according to assay.
Conclusion: Our findings demonstrate that the Gene Xpert MTB/RIF is able to accurately detect M. tuberculosis in pulmonary and extra-pulmonary specimens. The accurate and early diagnosis of TB infection allows timely therapeutic intervention, which is beneficial not only for the patient but also for possible contacts.
Masoomeh Rezanezhadi, Fahimeh Azadi, Shayan Marhamati, Hamid Reza Joshaghani,
Volume 17, Issue 6 (Nov-Dec 2023)
Abstract

Background: Herpes simplex viruses (HSV-1 and HSV-2) are a common cause of dermal, oral, and genital infections worldwide. Rapid diagnosis of infected patients will prevent transmission to uninfected individuals. Therefore, this study aimed to detect HSV-1 and HSV-2 by real-time polymerase chain reaction (RT-PCR) in patients from Golestan, Iran.
Methods: In this cross-sectional study, 645 suspected HSV patients hospitalized in the health centers of Golestan Province were selected between 2015 and 2020. Demographic data were collected, and DNA was extracted from the samples. Detection of HSV-1 and HSV-2 was performed by RT-PCR.
Results: Of the 645 samples tested by RT-PCR, 9 samples (1.39 %) were found to be positive for HSV-1/HSV-2, and the rest of the samples (98.6%) were negative. Of the 9 positive samples, 8 cases (88.8%) were HSV-1, of which 7 cases (77.7%) were from the cerebrospinal fluid (CSF) and 1 case (12.5%) from the vagina. HSV-2 was seen only in 1 vaginal specimen (11.1%).
Conclusion: Early diagnosis of these viral infections is very important as it can lead to early treatment and avoid further symptoms and even death. Thus, molecular approaches can provide an option for quicker diagnosis.

Page 1 from 1     

© 2007 All Rights Reserved | Medical Laboratory Journal

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.