Showing 5 results for Rafiee
S N Javid, E A Ghaemi, N Amirmozaffari, S Rafiee, A Moradi, T Dadgar,
Volume 3, Issue 1 (Spring - Summer 2009[PERSIAN] 2009)
Abstract
Abstract
Background and objectives: With almost nine million new cases each
year, tuberculosis is still one of the most Life-threatening diseases in the
World. Distribution of drug resistant strains of M.tuberculosis has a lot of
importance. This research was carried out to determine the frequency of drug
resistance of M. tuberculosis in strains isolated in Golestan province.
Material and Methods: In this cross -sectional study, 104 isolate of
M.tuberculosis which isolated from patients referred to Gorgan tuberculosis
Health Center, in 2008 were studied. DNA was extracted by Boiling Method.
By using PCR method, we determine the M.tubeculosis strain and resistance
to Rifampin (Using IS6110 and Gene rpoB primers) and resistance to
Isoniazid (Using InhA and KatG primers). As a Gold Standandard,
“Proportional method” was performed for 45 Samples.
Results: 87 strains were identified as M.tuberculosis. 6.9% of them were
resistant to Isoniazid, 4.6% to Rifampin and 2.3% to both (MDR).Sensitivity
and Specifity of PCR method in detection of resistant to Isoniazid were
95.3% and 57.1% and for Rifampin were 94.7% and 33.3%.
Conclusion: We found that in our region, the MDR is not very common.
More than 16% of isolated strains from tuberculosis suspected patients were
MOTT, for this reason it is necessary to mention that use biochemical or PCR
method to determine M.tuberculosis is necessary.
Key words: Mycobacterium tuberculosis, MDR, PCR, Proportional method
, Golestan province.
H Rafieemehr,
Volume 4, Issue 2 (Autumn – Winter 2011[PERSIAN] 2010)
Abstract
Abstract Bachground and objectives: Higher than needed blood orders not only adversely affect blood quality but also impose extra expenses on treatment center and patients. We aimed at determining the frequency of packed red blood cell transfusion in Besat hospital of Hamadan in 2009-2010. Material and Methods: This was a descriptive study on 926 blood order forms in Besat hospital. The data were collected using forms, in Hamedan teaching hospital (Besat) from March 2009 to March 2010.The amount of blood order, blood consumption, crossmatch /transfusion ratio(C/T) and transfusion index (TI) in different wards of hospital. Results: Out of 926 patients aged averagely 28 years, 37%were females and 63% are males. The overall ratio of C/T and TI are 2.44 and 0/63, respectively which are considered to be optimal in comparison with the standard figures of C/T<2.5 and TI≥0.5.The results show that the highest C/T is in surgery ward and the lowest in burn ward, but the highest TI is related to Hematology ward and the lowest to urology ward. Conclusion: Packed cell consumption, C/T Ratio and TI in Besat hospitals is normal but not ideal. It seems that absence of hospital blood transfusion committees and lack of active contribution of physicians, are the major obstacles in reforming blood utilization. Key words: Packed cell, Crossmatch, Transfusion Index
Sadeghi D (msc), Mosavari N (phd), Rafiee B (msc), Mohamad Taheri M (msc), Dashtipour Sh (bsc), Zare A (phd), Ghahremanlo E (msc), Tebyanian M (phd),
Volume 6, Issue 1 (spring-summer[PERSIAN] 2012)
Abstract
Abstract Background and objectives: Tuberculin is the proteins existed in tuberculosis culture medium which precipitated by trichloroacetic acid (TCA) or ammonium sulfate. Tuberculin is used for diagnosis of Tuberculosis. The aim of this study is to compare the human tuberculin produced by Razi Institute and Mycobacterium tuberculosis Culture Filtrate Protein. Material and Methods: Initially By biphasic medium, Bacteria from Lowenstein–Jensen solid medium was transferred to a Dorset−Henley Liquid medium. After 6 weeks of growth, the bacteria were isolated from liquid medium containing secretory proteins by the 0, 22 micron filter and the solution containing secretory proteins was precipitated by TCA and ammonium sulfate, separately. Then, using spectrophotometer and kjeldahl protein assay, the presence of protein in solution was confirmed. At the end, the precipitated proteins are compared with the human tuberculin by Coomassie-Blue stained SDS-PAGE Results: The protein samples precipitated by TCA have more bands in the limit of higher than 20 kDa, but the protein samples by ammonium sulfate have more bands in the limit of less than 20 kDa. Human tuberculin proteins are like smear and their weight is less than 16 kDa. Conclusion: It seems that ammonium sulfate is more suitable for low molecular weight proteins than TCA for precipitation. Key words: Mycobacterium tuberculosis, SDS-PAGE, tuberculin
Roya Rafiee , Fereshte Eftekhar , Seyed Ahmad Tabatabaei , Dariush Minaee-Tehrani ,
Volume 10, Issue 3 (May-Jun 2016 2016)
Abstract
ABSTRACT
Background and Objectives: Pseudomonas aeruginosa is the most frequent opportunistic pathogen isolated from the sputum of patients with cystic fibrosis (CF). Resistance to β -lactam antibiotics may arise from over expression of the naturally occurring AmpC cephalosporinases or acquired extended-spectrum β-lactamases (ESBL). The aim of this study was to determine the antibiotic resistance profiles as well as the prevalence of ESBL and AmpC production in clinical isolates of P. aeruginosa from CF patients in Tehran.
Methods: Antibiotic resistance of 50 non-duplicate P. aeruginosa isolates was determined by the disc diffusion method. AmpC β-lactamase production was detected by the antagonism disc test and ESBL production was detected by the phenotypic confirmatory test. The presence of ESBL and AmpC genes was assessed by PCR, followed by sequencing the PCR products.
Results: The antibiotic resistance rates were as follows: 22% to ceftriaxone, 20% to cefotaxime, 10% to imipenem, 8% to carbenicillin and 6% to ticarcillin, 4% each to cefepime, tobramycin, amikacin and aztreonam and 2% to each piperacilin, meropenem and ceftazidime. AmpC production was observed in 47 isolates (94%) and ESBL production was observed in one isolate (2%). PCR results showed that all isolates carried the blaAmpC β-lactamase gene. One multidrug-resistant isolate carried both blaTEM and blaPER-1 genes.
Conclusion: The results showed that despite the low rate of antibiotic resistance in P. aeruginosa CF isolates,the presence of multiple β-lactamases even in one isolate is alarming and can complicate the already difficult treatment of chronic infections in the lungs of CF patients.
Maryam Rafiee, Alijan Tabarraei, Mahsa Yazdi, Alireza Mohebbi, Ezzat Allah Ghaemi,
Volume 17, Issue 2 (Mar-Apr 2023)
Abstract
Background and objectives: Urinary tract infection (UTI) is one of the most common bacterial infections. Staphylococcus saprophyticus is a common Gram-positive bacterium that causes uncomplicated UTIs in women. The present study aimed to study the drug resistance pattern and phenotypic and genotypic variation of S. saprophyticus isolates from women with UTI in Gorgan, northern Iran.
Methods: This study was performed from May 2018 to September 2020. During this time, 35 S. saprophyticus strains were isolated from patients with UTI. The antimicrobial patterns of the isolates were determined by a conventional method. Phenotypic criteria such as pigment production, mannitol fermentation, urease production, and 16SrRNA gene valuation were studied.
Results: All isolates were sensitive to nitrofurantoin, gentamicin, and linezolid. S. saprophyticus isolates showed the highest level of resistance to penicillin (85.7%) and erythromycin (51.4%). A variation was detected among S. saprophyticus isolates in terms of pigment production i.e. about 51.4% showed yellow pigment in Muller Hinton agar, and 62.9% of the isolates were able to ferment mannitol sugar. Of 11 isolates that were sequenced for the 16SrRNA gene, only two isolates showed different patterns.
Conclusion: Nitrofurantoin and trimethoprim-sulfamethoxazole are the antibiotics of choice for the treatment of UTI caused by S. saprophyticus in the study area. Due to the phenotypic and genotypic differences among S. saprophyticus isolates, typing of S. saprophyticus at the subspecies level is recommended.
