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Photocatalytic Removal of Escherichia Coli by ZnO Activated by Ultraviolet-C Light from Aqueous Solution
Noroozi , R, Mehdinezhad, Mh, Zafarzadeh A,
Volume 5, Issue 2 (Autumn – Winter 2011[PERSIAN] 2011)
Abstract
Abstract: Background and objectives: There is a great interest in photocatalytic oxidation of contaminants, using ZnO, in recent years. The main objective of this research was to study photocatalytic disinfection of E. coli bacteria as water microbial pollution index, using nano particles of ZnO and a UV lamp in a batch reactor. Material and Methods: In this study, the contaminated water sample was prepared through adding 102 and 103 E. coli bacteria per ml of raw water. The contaminated water entered the photocatalytic oxidation reactor and removal efficiency of E. coli bacteria in different conditions were studied, including pH (5.5, 7, and 9), time (10, 20, 30, 40, 50 and 60 mins), dose of nano particles ZnO (0.2,0.4,0.8 and 1 gr/l), number of bacterium (102 and 103 per milliliter) and voltage of UV-C lamp, 27 volts. Characterizations of ZnO nanoparticles were determined using scanning electron microscope equipped with Energy Dispersive X-ray Analysis (EDX) system and X-ray diffraction (XRD) method. Results: Photocatalytic process efficiency is enhanced by increasing reaction time and dose of nano particles ZnO in the presence of UV lamp irradiation. The results show that the best conditions for removal of 102 and 103 bacteria per milliliter are obtained from condition including pH of 7, reaction time of 30 mins, 0.8 gr/l doses of nano particles ZnO and 27-volt-UV lamp. Conclusion: The results indicate that the increase of reaction time and dose of nano particles ZnO, in the presence of radiation UV lamp, have the most significant effect on photocatalytic efficiency. Based on the results, photocatalytic can be promising method for removal of E. coli bacterium from drinking water. Keywords: Photocatalytic, E. coli, irradiation UV, ZnO nanoparticles, water treatment
Antimicrobial effect of Stachys lavandulifolia Vachl essential oil on Listeria monocytogenes
Mohammadpourkanzaq, H, Noroozi, M, Mahmoudi, R, Mohammadpoorasl, A, Zavoshy, R, Asadinadari, M,
Volume 9, Issue 2 (may,jun 2015[PERSIAN] 2015)
Abstract

Abstract

Background and Objective: It has been proved that plant essential oils have antimicrobial effects. Stachys Lavandulifolia Vachl is a medicinal plant growing wild in many parts of Iran, and is used as a brewed drink to treat some diseases.

Material and Methods: Aerial parts of Stachys lavandulifolia Vachl at flowering were collected from the Sabalan mountainous   area of Ardabil and its essential oil was extracted using a Clevenger-type apparatus. A GC/MS machine was used to identify the chemical constituents of this Essential oil. We used microdilution method to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Essential oil against Listeria Monocytogenes ATCC19118 bacteria.

Result: Sixteen chemical compounds were identified in this essential oil. Of these, γ-terpinene (28%), Phenol (18.16%), Myrcene (17.87%), and α-Pinen (12.7%) were the major ones. The MIC and MBC of the essential oil for Listeria Monocytogenes bacteria were 600 and 2400 ppm, respectively.

Conclusion: Results showed that the Monoterpene and Sesquiterpene groups are the main constituents of this essential oil having bactericidal effects against Listeria Monocytogenes bacteria.

Keywords: Essential Oil, Stachys Lavandulifolia, Listeria Monocytogenes


Frequency of H.Pylory BabA2 and Hpa Genes in Patients with Dysphagia
Khatoon Heydari, Ramin Azarhoosh, Vahideh Kazeminejhad, Fatemeh Shakeri, Alireza Noroozi,
Volume 9, Issue 5 (Nov,Dec-2015 2015)
Abstract

Abstract

      Background and Objective: BabA2 and Hpa genes are involved in adherence of Helicobacter pylori (H.pylori) to gastric mucosal tissue. This study aimed to investigate the frequency of these genes in isolates of H. pylori from gastric biopsies and their relationship with gastritis, peptic ulcer and gastric cancer.

      Methods: Gastric biopsy samples were obtained from patients with gastritis, peptic ulcer and gastric cancer. A sample was sent to the laboratory for urease test and histopathology study, and another sample for DNA extraction. The frequency of BabA2 and Hpa genes was investigated using their specific primers by PCR.

      Results: Among the 80 analyzed biopsy samples, 51 (63%) were BabA2 positive, and the frequency of this gene in the samples of gastric cancer, gastritis and peptic ulcer was 61.1, 58.3 and 73.3%, respectively. In addition, 57 samples (71%) were Hpa positive, and the frequency of this gene in the samples of gastric cancer, gastritis and peptic ulcer was 55.5, 69.4 and 84.6%, respectively. There was no significant correlation between the presence of these genes and the type of H.pylori-related diseases.

       Conclusion: Frequency of BabA2 and Hpa genes is higher in the samples of peptic ulcer but there was no significant relationship between these genes and H.pylori-related diseases.

      Keywords: BabA2, Hpa, Gastric Cancer, Gastritis, Peptic Ulcer.


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