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Showing 3 results for Lotfi
Serum Alpha-1-antitrypsin Evaluation by Three Electrophoretic, Enzymatic and Immunodiffussion Methods
M Mohammadian Yajloo,, A Sahebgadam Lotfi, M Nasroallahzadeh Sabet, N Zhalehjoo, M Amirian, M Biglarzadeh,
Volume 1, Issue 1 (Spring - Summer 2007 [PERSIAN] 2007)
Abstract

Abstract Background & Objective: Alpha-1-antitrypsin (AAT) is the major component of the human plasma alpha-1 globulin proteins and acts as a major inhibitor of proteolytic enzymes, particularly elastase. AAT deficiency is accompanied by lung, liver and other disorders, therefore, AAT is clinically important and its precise evaluation is diagnostically critical. In present study serum AAT was evaluated by three Cellulose Acetate Electrophoresis (CAE), Trypsin Inhibitory Capacity (TIC) and Single Radial Immunodiffusion (SRID) methods and results wene compared. Materials and methods: AAT evaluation was carried out, by CAE, SRID and TIC Methods, on 318 normal sera obtained from volunteer students of Tehran Universities. Results:The results indicated: 34, 84 and 112 samples by TIC, SRID and CAE methods (with reference ranges of 2.1-3.5 mol/min/ml, 126- 226 mg/dl and 2-4.5% respectively) were abnormal 201samples by CAE and TIC were normal and 29 abnomal, 83 sera were normal by TIC and abnormal by CAE five of them were abnormal by TIC and normal by CAE 227 of the samples were normal and 29 abnormal(TIC and SRID) 57 were normal by TIC and abnormal by SRID and seven samples were abnormal by TIC and normal by SRID. Conclusion: Although CAE and alpha-1 globulin band determination are routine in clinical laboratory, they are not reliable in evaluating AAT. SRID sensitivity is more Than CAE and less Than TIC therefore, TIC is recommended as a precise and reliable method for serum AAT evaluation. Key Words: Alpha-1-antitrypsin, Cellulose Acetate Electrophoresis, Single Radial Immunodiffusion, Trypsin Inhibitory Capacity


Preparation and Characterization of a Neutralizing Monoclonal Antibody against Poliovirus Type 1 (Mahoney Strain)
Sina Soleimani , Morteza Kamalzadeh , Mohsen Lotfi ,
Volume 13, Issue 1 (Jan-Feb 2019)
Abstract
ABSTRACT
          Background and Objectives: Poliomyelitis remains a major public health problem in developing countries, which signify the need for extensive diagnostic and prevention research. The aim of the present study was to design monoclonal antibodies (MAbs) against poliovirus type I with biomedical, diagnostic and therapeutic applications.
          Methods: B-cells were isolated from a mouse challenged with polio antigen injection. The B-cell were fused with myeloma tumor cells. After evaluation and screening of approximately 250 hybridoma colons by ELISA, 35 colons with the highest antibody titer and no cross-reactivity were selected and subsequently cloned by limiting dilution. Finally, three colons capable of secreting MAbs against epitopes of poliovirus type I were used for MAb production. Next, the MAbs were characterized by antibody assays, isotyping, epitope analysis (western blot), cross-reactivity test, stability test, sterility test and mycoplasma test.
          Results: The results indicated that the MAbs were of IgG1 kappa chain, had good stability and no cross-reactivity. In western blot, a band at 26 kDa which is associated to VP3 neutralization protein was observed.
          Conclusion: These serotype-specific MAbs can be potentially used for identification of type I poliovirus for research, diagnostic and prevention purposes.
          Keywords: Monoclonal antibody, Hybridoma, Poliomyelitis, Poliovirus.

Efficacy of Beetroot Juice Consumption on the Lipid Profile of Female Soccer Players
Maryam Lotfi, Mohammad Azizi, Worya Tahmasebi, Parviz Bashiri,
Volume 14, Issue 2 (Mar-Apr 2020)
Abstract
ABSTRACT
            Background and Objective: It is desirable for athletes, especially female soccer players, to consume beetroot beverage during training as a way of improving both health and performance ,. Therefore, the objective of this study was to  assess the effect of beetroot juice intake on lipid profile in female soccer players.
            Methods: Young and well-trained female soccer players (N=20) were  included in a randomized placebo-controlled crossover study. They were categorized into two groups: experimental (beetroot beverage, n=10, age: 23.20±0.91 years, BMI: 22.57±1.54(kg/m2)) and control (placebo, n=10, age: 23±0.81 years, BMI: 23.13±0.58(kg/m2)). Subjects drank either 200 ml concentrated beetroot beverage or an equal amount of placebo 2 hours prior to their trainings for six weeks (18 session). Blood samples were taken 24 hours before and after trials. All data were compared in pretest and posttest  via paired sample t-test and independent sample t-test. All statistical analyses were performed at (P≤0.05) by SPSS (version22).
            Results: Beetroot juice intake increased plasma high density lipoprotein (t= -60.88, P<0.05). Triglyceride, total cholesterol, and low density lipoprotein were  reduced (P<0.05). Compared with placebo, beetroot  juice reduced the concentrations of triglyceride, total cholesterol, and low density lipoprotein (P<0.05).
            Conclusion: Regular beetroot juice intake has significant effects on lipid profile in female soccer players, hence its suggestion for preventing diseases such as  hypercholesterolemia and  hypertension in female soccer players.
            Key words: Soccer, Training, Dietary Supplements, Lipids.

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