Showing 2 results for Kazemi, A
Saadat, S, Solhjoo, K, Norouz-Nejad, Mj, Kazemi, A, Erfanian, S, Ashrafian, F,
Volume 8, Issue 4 (supplement Issue[PERSIAN] 2015)
Abstract
Abstract Background and Objective: Vancomycin is used for treatment of methicillin-resistant S. Aureus (MRSA) infections therefore, resistance to this antibiotic is increasing. We aimed to determine the antibiotic resistance pattern and frequency of vancomycin resistant S. Areas (VRSA) strains isolated from clinical samples. Material and Methods: In this cross-sectional study, 100 S. Aureus isolates collected from hospitals in Shiraz during six months, 2012, were identified by biochemical, microbiological and molecular methods. After determination of antibiotic susceptibility pattern by disc diffusion method and vancomycin agar screening test, Minimum Inhibitory Concentration (MIC) was determined by E-test for vancomycin, thicoplanin, linezolid and quinupristin-dalfopristin. Results: The most resistant and the most sensitive antibiotic were ampicillin (%95) and quinupristin-dalfopristin (99%), respectively, and 44% of isolates were resistant to methicillin. In agar screening test, 48% of strains had reduced sensitivity and in disc diffusion 3% strains were resistant to vancomycin. In E-test method, only one isolate was resistant to vancomycin. Conclusion: given the presence of VRSA and new antibiotic resistant strains, we recommend doing some intervention to prevent from spreading these strains in hospitals. . Keywords: Clinical Specimens, Staphylococcus Aureus, Vancomycin, Antibiotic Resistance
Zare, Z, Solhjoo, K, Norooznejad, Mj, Kazemi, A,
Volume 8, Issue 4 (supplement Issue[PERSIAN] 2015)
Abstract
Abstract
Background and Objective: Increasing prevalence of methicillin resistant Staphylococcus aureus strains (MRSA) with their multidrug resistance potential causes difficulties in the treatment of infections due to these bacteria. Hence, the detection and determination of the frequency of MRSA strains via phenotypical and molecular methods is necessary in different parts of the county.
Material and Methods: In this cross- sectional study, 150 Staphylococcus aureus strains were collected from different clinical samples in the hospitals located in Shiraz and Jahrom, Iran. To detect methicillin resistant Staphylococcus aureus strains, we used phenotypical methods such as disc diffusion and minimum inhibitory concentration by E-Test, and PCR molecular method for mass gene.
Results: The frequency of methicillin resistant Staphylococcus aureus was 63 strains (42%) using disc diffusion and E-Test. while in PCR method, in addition to 63 strains, nine other isolates, which were sensitive to oxacillin by disc diffusion and E-Test, possessed also mecA gene. By and large, 72 isolates (48%) had methicillin resistance gene.
Conclusion: Given the results of phenotypical and molecular methods, the frequency of methicillin resistant Staphylococcus aureus was relatively high in this area. Thus, the MRSA strains can be detectable as soon as possible by accurate and sensitive methods such as PCR to determinate the effective antibiotics.
Keywords: Methicillin Resistant Staphylococcus Aureus, MRSA, MecA Gene, PCR
