Search published articles


Showing 4 results for Jamalli
Impact of Various Environmental and Growth Conditions on Antigen 43 Gene Expression and Biofilm Formation by Uropathogenic Echserchia coli
Mohammad Arjmand , Ezatallah Ghaemi , Ailar Jamalli ,
Volume 11, Issue 1 (Jan-Feb- 2017 2017)
Abstract
ABSTRACT
        Background and Objectives: Biofilm is a population of bacteria growing on a surface and enclosed in an exopolysaccharides matrix, which increases resistance to antimicrobial agents and immune response. Uropathogenic Escherichia coli (UPEC) are biofilm-forming bacteria and the most common cause of urinary tract infections (UTIs). This study evaluated the effect of different concentrations of glucose, NaCl, blood, serum and urine on biofilm formation and antigen 43 (Ag43) gene expression, as a main gene involved in biofilm formation.
        Methods: Among E. coli isolates from patients with UTI, four extended-spectrum beta-lactamase (ESBL) and non-ESBL strains, and a standard UPEC strain were selected. Biofilm formation of the strains in brain heart infusion (BHI) broth with different concentrations of glucose, NaCl, sheep blood, serum and human urine was evaluated using microplate method and crystal violet staining. Ag43 gene expression was investigated using Real-Time polymerase chain reaction, SYBR Green dye, and specific primers.
           Results: Presence of glucose at all concentrations reduced biofilm formation. Presence of 1% NaCl, 1% sheep blood, 10% bovine serum, and 5% urine significantly increased biofilm formation. Expression of Ag43 by the strains grown under 1% glucose, 1% NaCl, 1% sheep blood, 10% bovine serum and 5% urine decreased.
         Conclusion: All environmental factors other than glucose may increase biofilm formation of E. coli at different concentrations. This is not affected by factors such as isolation from inpatient or outpatients and type of strains (ESBL or non-ESBL). Contrary to our expectations, Ag43 expression is independent of environmental factors and decreases even under the most suitable concentrations.
          Keywords: Biofilms, Uropathogenic Escherichia coli, UTI, Antigen 43, Real-Time PCR.

Efficacy of Mupirocin Ointment in Eradication of Staphylococcus aureus Nasal Carriage in Intensive Care Unit Staff and Patients
Abolfazl Khandan Del , Ania Ahani Azari , Ailar Jamalli, Ezzat Allah Ghaemi,
Volume 12, Issue 3 (May-Jun 2018)
Abstract
ABSTRACT
          Background and Objectives: Staphylococcus aureus is one of the most common causes of morbidity and mortality among intensive care unit (ICU) patients. Nasal carriage is one of the main routs of S. aureus transmission between hospital personnel and patients. The objective of this study was to evaluate the efficacy of mupirocin ointment in eradication of nasal carriage of S. aureus in the ICU staff and patients of Panje-Azar hospital in Gorgan, Iran.
          Methods: In the first three months of the study (January to March), the prevalence of S. aureus among ICU patients was determined by routine microbiological and biochemical testing. Nasal samples were taken from ICU staff and all patients recently admitted to the ICU. Mupirocin nasal ointment (2%) was applied for treatment of S. aureus nasal carriers. Post-treatment sampling was done after five weeks. During the next three months, the presence of S. aureus and rate of resistance to methicillin was evaluated in new patients admitted to the ICU using the method used previously.
          Results: Of 60 samples from the ICU staff, seven (11.7%) samples were positive for S. aureus. Moreover, of 240 samples from the ICU patients, two samples were found as S. aureus-positive. Of the nine S. aureus-positive isolates, only two (22.2%) were methicillin-resistant S. aureus (MRSA). In the pre-intervention sampling, only five samples (2.8%) were identified as S. aureus, two of which were MRSA. However, treatment with mupirocin ointment eradicated nasal carriage of S. aureus and no isolate was found after the intervention.
          Conclusion: Our finding showed that mupirocin nasal ointment is highly effective in eradication of S. aureus nasal carriage and subsequently contribute to reduction in frequency of nosocomial infections in the ICU.
          Keywords: Intensive Care Units, Mupirocin, Nasal, Staphylococcus aureus.

Evaluation of Biofilm Formation in Staphylococcus aureus Clinical Isolates
Abolfazl Khandandel , Hami Kaboosi , Ailar Jamalli , Fatemeh Peyravii Ghadikolaii ,
Volume 13, Issue 4 (Jul-Aug 2019)
Abstract
          Background and Objectives: Staphylococcus aureus is a common cause of nosocomial infections. The ability of S. aureus to form biofilm and acquire antimicrobial resistance has made this organism a major health problem. In this study, we investigate the biofilm-forming ability of S. aureus isolates from clinical samples.
          Methods: Sixty S. aureus isolates from clinical specimens were collected from the 5th Azar Hospital of Gorgan (Iran) in 2018. The isolates were identified using conventional methods including Gram staining and biochemical tests (catalase and coagulase). Biofilm formation by S. aureus isolates was evaluated using a microplate-based method.
          Results: Of 60 S. aureus isolates, 47 (78.3%) strains were identified as biofilm-forming and 13 (21.7%) strains were non-biofilm-forming.
          Conclusion: The high prevalence of biofilm-producing S. aureus isolates in the 5th Azar hospital of Gorgan could pose a major health challenge with serious consequences for hospitalized patients. Therefore, it is crucial to disinfect and sterilize hospital surfaces and equipment effectively to minimize the risk of contamination and spread of bacteria in the hospital settings.
          Keywords: Biofilms, Staphylococcus aureus, sample.

Prevalence of Biofilm Formation and Detection of PSM B Gene in Clinical Isolates of Staphylococcus aureus
Ali Asghar Ayatollahi, Abolfazl Khandan Del, Ailar Jamalli, Khashayar Shahin, Ania Ahani Azari,
Volume 14, Issue 1 (Jan-Feb 2020)
Abstract
Background and objectives: Staphylococcus aureus is a frequent cause of hospital- and community-associated infections on a global scale. This organism is responsible for causing an extensive range of diseases and many of them are capable of biofilm formation for their survival. By this method, treatment of them with antibiotics become very difficult and antibiotic resistance is another rising concern.
Material and Methods: The clinical samples were collected and examined for Staphylococcus aureus by microbiological and biochemical tests. Then, the biofilm formation in Staphylococcus aureus isolates was detected by microtiter plate. Using SYBR Green Real-Time PCR, the expression of PSM was determined.
Results: A total of 60 strains of Staphylococcus aureus were isolated from clinical isolates. Of them, 47 strains (78.3%) were identified biofilm producing and the others were considered negative for biofilm formation. After real-time PCR testing to detect PSM, it was determined that 100% of the strains were positive for biofilms and PSM genes. The results of phenotypic and genotypic tests of biofilm were closely related to each other and the expression of PSM B gene was 80%. It was found that 100% of strains were biofilm producing and PSM B gene was present in 78.3% (47 strains) of them.
Conclusion: The prevalence of biofilm production in Staphylococcus aureus strains isolated from clinical samples was high, so it is highly important to monitor the prevalence of these organisms in hospitals and community as well as their antimicrobial resistance.
Page 1 from 1     

© 2007 All Rights Reserved | Medical Laboratory Journal

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.