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Showing 3 results for Imani Fooladi

M Sattari, Aa Imani Fooladi, Gh Godarzi,
Volume 3, Issue 1 (Spring - Summer 2009[PERSIAN] 2009)
Abstract

Abstract Background and objectives: Pseudomonas aeruginosa as an opportunistic pathogen can establish lethal infections in immunocompromised patients or those exposed to predisposing factors. This bacterium contains a single polar flagellum causing motility, chemotaxis and colonization in acute phase of infection. The flagella filament is made up of a structural protein called flagellin. This study was aimed at determining The frequency of fliC gene in Clinical Samples. Material and Methods: In this study, a pair of specific primer for types of flagellin (a, b type) was designed and by using PCR method its structural gene (fliC) was recognized and amplified in clinical strains. Results: This original primer has appropriate efficiency in diagnostic of pseudomonas aeroginosa flagellum. Our study shows that 85% of the Clinical Samples have a fliC gene. Conclusion: This method can be applied to recognizing of the motile strains, and their antigenic typing, and complete amplification of fliC sequence in order to cloning and expression of recombinant flagellin. Key words: Pseudomonas aeruginosa, flagellin, fliC, PCR
Sultanpour M J, Imani Fooladi A A, Bagherpour G, Malek M,
Volume 5, Issue 1 (spring-summer[PERSIAN] 2011)
Abstract

Abstract Background and objectives: Anaerobic microorganisms, particularly bacteroides play an important role in causing Pulp and preapical diseases that lead to chronic abscess. we aimed at studying anaerobie infections in necrotic teeth having Acute and chronic clinical symptoms. Material and Methods: We examined 28 necrotic teeth of 28 patients for the purpose of anaerobic culture fifteen cases with acute clinical symptoms and 13 ones with chronic clinical symptoms. In total 38 root canals from 28 teeth were subjected for examination and sampling. The samples were cultured on thioglycolate medium, sent to laboratory and tested in anaerobic condition for identification of bacteria. Results: The results indicate that 76% of isolated bacteria from necrotic canals obligate anaerobes and 24% are facultative anaerobes. The most common isolated microorganisms are peptostreptococci, fusobacterium and bacteroides. Conclusion: These results are in agreement with the results of other researchers and it has been determined that bacteroids play an important role in destroying the necrotic teeth's bone. Anaerobic bacteria causing dental infactions are less identified and diagnosed, therefore, dentist and clinical lab should take this into account. Key words: Anaerobic Bacteria, Necrotic tooth, Pulp, preapical
T Dadgar, E Ghaemi, N Bahador, A Imani Fooladi, F Kamareie,
Volume 7, Issue 5 (supplement Issue( Bacteriology)[PERSIAN] 2014)
Abstract

Abstract Background and Objective: The main cause of spreading staphylococcal infections among patients is the healthy carriers working in hospitals. With the secretion of different sorts of toxins such as entrotoxin, this bacteria can provide the conditions for attacking on the host. The main objective of this study is identification of the characteristics and differences in the Staphylococcus aureus isolated from healthy carriers and from the patients on the basis of enterotoxin genes (sea-see). Material and Methods: One hundred and twenty of the patients and 80 of healthy carriers worked in health centers of Gorgan, north of Iran, were investigated for S. aureus isolate. The isolates were evaluated by PCR for Enterotoxin Genes A-E (SEA to SEE). Results: Enterotoxin genes (SEA to SEE) was found in 87.5% of the total isolates and the most frequent one was enterotoxin gene sea (N= 124). The prevalence of these isolates in healthy carriers was significantly higher than those of the patients. Conclusion: Based on the results, the high percentage of S. aureus isolated from clinical samples contains enterotoxin genes. Therefore, Human as the source and carrier of S. aureus is paramount importance, which is due to significant relationship between being toxigenic strains and the source of isolation. Key words: Staphylococcus Aureus Enterotoxin Patient Carrier

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