Showing 15 results for Ghasemi
F Ghasemi Kebria, B Khodabakhshi, H Kouhsari, M Sadeghi Sheshpoli, N Behnampoor, S Livani, M Bazuori, E A Ghaemi,
Volume 4, Issue 1 (Spring - Summer 2010[PERSIAN] 2010)
Abstract
Abstract Background and objectives: After respiratory infection, Diarrhea is the second cause of mortality. Yersinia enterocolitica is the second important cause of infectious diarrhea in children of some countries. In this study, we evaluated the frequency of Yersinia entocolitica of diarrheal specimens in Gorgan, Iran. Material and Methods: This descriptive cross - sectional Study was carried out on diarrheal stools of 455 patients referred to medical centers and laboratory of Gorgan in 2004-2005. DNA extraction using phenol chloroform was performed for all samples. Using two specific primers (genus-specific16s rRNA and ail- specific species genus of Yersinia enterocolitica), we did PCR sample. Results: Yersinia genome was identified in 12 patients(2.63%) and 11 of them was Yersinia enterocolitica. The frequency infection in of girls (3%) was more than boys (2.4%), and the prevalence in winter (4%) was more them other seasons, and under one- year- group (3.4%) and 1-5 years (3.1%) is more than other age groups. It was not observed significant difference. (P> 0.05). Conclusion: The frequency of Yersinia in cases of diarrhea in Gorgan is similar to most regions of Iran and in children under 5 years is observed more in winter. Key words: Yersinia enterocolitica, Diarrhea, children, Gorgan
Sh Semnani, Gh Roshandel, H Faghih, F Ghasemi Kebria, H Joshghani,
Volume 7, Issue 4 (winter[PERSIAN] 2014)
Abstract
Abstract
Background and Objective: The patients with Post-Infectious Irritable bowel syndrome (PI-IBS), a subtype of irritable bowel syndrome, suffer from bacterial gastroenteritis. Since campylobacter Jejuni (CJ) is one of the most common agents in this syndrome, we aimed to evaluate the prevalence of Campylobacter Jejuni in patients with Irritable Bowel Disease.
Material and Methods: This case - control study was conducted on 160 patients divided into 2 equal groups of healthy and unhealthy. The presence of Anti- CJ antibody (IgG and IgA) was evaluated by ELISA and the comparison was performed by chi-square test.
Results: The mean age of case (31.51) and control (31.84) was not statistically different (P = 0.87). Titer of Anti- Campylobacter Jejuni antibody IgG was positive in 25% of patients and 18.8% of the healthy ones (p=0.02). IgA Seropositivity in patients was 7.5% but no one in control group was positive (p =0.01)
Conclusion: Seroprevalence of Cj in patients with IBS was higher significantly than that of control group. Thus, Cj can be known as one of the causes of Post-infection in patients with IBS in our region and it should be paid more attention in diagnostic assessment of these patients.
Keyword: Irritable Bowel Syndrome Campylobacter Jejuni Antibody
R Golsha, V Kazeminejad, A Barzegari, S Besharat, F Ghasemi Kebria,
Volume 7, Issue 5 (supplement Issue( Bacteriology)[PERSIAN] 2014)
Abstract
Abstract
Background and Objective: The excessive use of broad-spectrum antibiotics will lead to drug resistance of microorganism and specially nosocomial organisms. Because of high incidence of antibiotic resistance in hospitals, we aimed to study antibiotic resistance to gram negative bacteria.
Material and Methods: This cross-sectional study was conducted on the data of biological samples (2006-2008), with positive culture result. Using antibiogram, microbial resistance to isolated microorganism was determined, and after culturing the samples, bacteria were identified by using differential media and antiserum. Then, antibiotic resistance was performed by disk diffusion.
Results: The most common gram-negative microorganism obtained from all cultures was Ecoli with the lowest drug resistance to Nitrofurantoin.
Conclusion: Based on the results, antimicrobial resistance pattern is not the same in different places and furthermore it is ever changing. Therefore, further research is needed to be done to have an accurate pattern of antibiotic resistance to provide effective treatment regimens.
Key words: Antibiotic Resistance Disk Diffusion Gram Negative Bacteria Gorgan
M Rezaei, R Ghasemi Khah, M Parviz, O Zarei,
Volume 8, Issue 2 (summer 2014[PERSIAN] 2014)
Abstract
Abstract Background and Objective: Ice cream is a suitable environment for microbial growth due to its chemical structure, ingredients, and its increased supply and demand. In the absence of hygienic considerations, it can cause poisoning. This study aimed to determine bacterial contamination in traditional ice cream produced in Arak city in 2011. Material and Methods: The samples (n= 30) were randomly obtained from different parts of Arak in, 2011. The Samples were shipped in cold conditions and total count of microorganisms test was performed according to Iranian national standards. Results: In 16.66%, the microbial contamination was below the limit of microbial load (5×104), and in 83.3% the contamination was more than allowed level. Conclusion: This study highlights the dire situation for bacterial contamination of traditional ice cream in Arak city. Keywords: Arak, Ice Cream, Microbial Contamination
A Zafarzadeh, N Amanidaz, N Seyedghasemi,
Volume 8, Issue 3 (Autumn[PERSIAN] 2014)
Abstract
Abstract
Background and Objective: Safe drinking water is essential for health and health promotion is dependent on providing safe water. We aimed to determine the relationship between turbidity & residual chlorine and microbial quality of drinking water in Agh ghala.
Material and Methods: In this descriptive-analytical study, 2079 water samples were collected from water networks of 78 villages and urban network using census sampling during two years. Both sampling and tests were performed on the basis of standard methods.
Results: In more than 96 percent of the villages (N =75), above 90% of the samples hadn’t any fecal coliform bacteria except three villages that had the index in the range of 85 to 88 percent. Residual chlorine had significant relationship with coliform and fecal coliform (P ≤ 0.05) while the relationship between turbidity and coliform contaminants, fecal coliform and residual chlorine was not significant.
Conclusion: Total coliform and fecal coliforms were reducing by increasing residual chlorine in the water supply networks. It has been suggested that the officials reduce the water turbidity and annual washing of the water network to increase the effect of residual chlorine and decrease bacterial contamination.
Keywords: Agh Ghalla, Fecal Coliform, Coliform, Residual Chlorine
M Elmi Merian, M Faezi Ghasemi,
Volume 8, Issue 3 (Autumn[PERSIAN] 2014)
Abstract
Abstract
Background and Objective: Burkholderia cepacia complex (BCC) is a plant pathogen that is an important mortality factor in immune-compromised and hospitalized patients. We aimed to Isolate and Characterize the Burkholderia Cepacia Strains from Hospitalized Patients in the Hospitals of West Guilan Province.
Material and Methods: This study was conducted on 90 saliva and blood samples obtained from patients with blood infection, pneumonia, asthma, patients connected to the monitoring and ventilator systems, and immune-compromised patients in different sections of hospitals such as the pediatrics, internal section, ICU and CCU. Primary screening was performed by cultivating the samples on Burkholderia cepacia selective agar (BCSA) Sensitivity to antibiotics was tested by Kirby-Bauer and Muller-Hinton Agar (MHA) and the separated isolations were recognized by strengthening the gene rec A.
Results: Of 90 isolated samples, only one strain suspected B. cepacia was isolated from 24-year old women with asthma. This strain was resistant to bacitracin, pipracillin and ciprofloxacin antibiotics.
Conclusion: The incidence of B. cepacia is rare in western part of Guilan province, which is congruent with the results of overall incidence in Iran.
Al Barari, Aa Ayatollahi, S Shirali, M Ghasemi, A Hosseini, Ar Ekrami, A Khandan Del,
Volume 8, Issue 3 (Autumn[PERSIAN] 2014)
Abstract
Abstract Background and Objective: The hormonal responses are different, according to type, intensity and the duration of training. We aimed to compare the effect of endurance and resistance training in untrained men on the level sexual hormone including testosterone, estradiol, and on sex hormone binding globulin (SHBG). Material and Methods: this study was conducted on male Student Volunteers divided randomly into three groups of eight members, including endurance, resistance and control. To measure the variables, we performed sampling before and after the training program. Results: the levels of testosterone and estradiol, in both groups have a significant (p<0.05) increase and decrease respectively. SHBG level was significantly increased only in endurance group (p<0.05), But LH level was meaningfully increased only in the resistance group (p<0.05). Conclusion: The endurance and resistance training can increase the production of testosterone .Increased testosterone and decreased estradiol in men lead to sperm production and improve fertility. Keywords: Endurance training, Resistance Training, Testosterone, Estradiol
Soltan Dallal, Mm, Rahbar, M, Douraghi, M, Rahimi Forooshani, A, Khan Babaei, Gt, Mobarhan, M, Ghasemi, F,
Volume 8, Issue 5 (winter[PERSIAN] 2015)
Abstract
Abstract
Background and Objective: Cystic fibrosis (CF) is an autosomal recessive genetic disease and Pseudomonas aeruginosa is one of the most common bacteria colonized in CF patients. Growing resistance of this bacterium to antibiotics now a day is a challenge of controlling infection in CF patient. In this study colonization of CF patients with Pseudomonas aeruginosa and antibiotic susceptibility pattern of isolated strains were examined.
Material and Methods: From 100 CF patients, during a year, sputum and bronchial swabs were collected. After culturing the samples, some of them were reported as Pseudomonas aeruginosa using biochemical tests. Mucoid strains of Pseudomonas aeruginosa were identified the same as non-producing alginate strains while for catching single pure colony, repeated passage was used. For determining antibiotic resistance of Pseudomonas aeruginosa to some antimicrobial agents Kirby-Bauer method based on CLSI was used.
Results: Of 100 samples, 40 (40%) were positive for Pseudompnas aeruginosa. The prevalence of P. aeruginosa was 23.8, 36.84 and 80% at the age of 1-3, 4-12 and 13, respectively.
Conclusion: Statistically, there is a significant difference between age and contracting with Pseudomonas aeruginosa in that the higher the age the more colonization with Pseudomonas aeruginosa.
Key words: Pseudomonas Aeruginosa, Cystic Fibrosis, Drug Resistance
Mohammad Taghvaie, N, Jalali, Mt, Ghasemi Falavarjani, M, Shahbazian, Bb, Saki, A,
Volume 9, Issue 2 (may,jun 2015[PERSIAN] 2015)
Abstract
Background and Objective: According to recent changes in diagnostic criteria for diabetes, the harmonization of results obtained from various methods and systems by considering their accuracy and precision is essential. This study aimed to evaluate the accuracy, precision and consensus of some routine laboratory glucose kits in comparison with Hexokinase reference method.
Material and Methods: The participants were 38 diabetic patients with fasting blood sugar (FBS) ≥126 mg/dl, nine prediabetic patients with FBS of 100-125 mg/dl, 15 non-diabetic people with FBS of 60-100 mg/dl and 9 hypoglycemic patients with FBS of ≤60 mg/dl. Their FBS were measured by four routine laboratory glucose kits: Glucose oxidase on BT3000 analyzer with an open system and Hexokinase reference method on a close system (COBAS INTEGRA®400plus analyzer, Roche kit). Accuracy and precision were determined and compared with reference method.
Results: Glucose oxidase methods showed a good agreement with the reference method, in Correlation Coefficient>0.99. based on regression analysis, the slope of 1.114 for Pars Azmoon, 1.105 for Bionik, 1.121 for Elitech and 1.087 for Human were reported (P<0.05). Error of the mean for ParsAzmoon was 12.79, for Bionik 10.86, for Elitech 12.58 and for Human were 8.46. Coefficient of Variation showed more imprecision for Bionik and Human kits.
Conclusion: given the same almost standard errors, standard devisions and regression analysis, the precision in four methods is the same but in comparison with Hexokinase, reference method has not the accuracy.
Keywords: Blood Glucose, Glucose Oxidase, Hexokinase, Methods, Consensus
Sadeghi, M, Amini, A, Behnampour, N, Ghasemi, Sm, Arefian, A., Mohajer, S,
Volume 9, Issue 2 (may,jun 2015[PERSIAN] 2015)
Abstract
Background and Objective: Staphylococcus aureus is an important cause of food poisoning. Due to the high consumption of fruit and ice cream in summer, this study aimed to determine the prevalence of S. aureus in traditional ice cream and juice in Gorgan, Iran.
Material and Methods: This cross-sectional study was conducted on 25 places selling traditional ice cream and juice in the summer of 2014 in Gorgan, Iran.
Results: Results showed that the microbial contaminants in traditional ice cream and juice centers were 56% and 14.28% of S. aureus, 44% and 33.3% of yeast, 28% and 28.57% of B. cereus, 12% and 4.76% of Listeria, 8% and 14.28% of Bacillus subtilis and 4% and 4.76% of Bacillus licheniformis ,respectively.
Conclusion: The findings of this study indicated a high contamination of traditional ice cream and juice with Staphylococcus aureus and various microorganisms.
Keywords: Ice Cream, Food Contamination, Staphylococcus Aureus
Azadi F, Hedayat Mofidi, M , Ali Arab, A , Hedayat Mofidi, H , Khorasaninjad, R , Ghasemi-Kebria, F , Roshandel, Gh ,
Volume 9, Issue 3 (Jul,Aug2015[PERSIAN] 2015)
Abstract
Background and Objective: Helicobacterpylori(HP) is the most common bacterial infection in the world and it may be associated with chronic gastritis, pepticulcer disease, and gastricadenocarcinoma. The aim of this study was to determine the prevalence of HP in student of Golestan University of Medical Sciences (GOUMS).
Material and Methods: This cross-sectional study was conducted on students ofGOUMS in 2012. Anti-HP IaG and IgA were assessed byELISA method.
Results: The participants recruited were 287 in that 101 (35.2%) were male and the rest female. Of 287 , 239 (83.3%) were positive for HP. The proportion of men was 91.1% and women 79% (P =0.03). There was no significant relationship between HP infection and variables such as age, blood group , place of residence, nativity, and level of education.
Conclusion: The prevalence of H. pylori infection in the students of Golestan University of Medical Sciences is high.
Behzad Ghasemi, Mohsen Najimi, Hamid Beyzaei, Abbas Jamshidian,
Volume 9, Issue 4 (sep,Oct 2015 2015)
Abstract
Abstract
Background and Objectives: Antibiotic resistance in bacteria has actuated researchers toward evaluating many new antibacterial compounds of which are the thiazoles. In this research the inhibitory effects of novel thiazole derivatives were unraveled on Staphylococcus aureus, Streptococcus agalactiae, Pseudomonas aeruginosa and Klebsiella pneumoniae and oxothiazole liver toxicity effects were assessed on mice.
Methods: The antibacterial effect of thiazole derivatives was evaluated by measuring the halo zone with disk diffusion method and dilution procedure in microplate in order to discriminate the minimum inhibitory concentration (MIC) and the liver toxicity of oxothiazole, also, was discerned by injecting 160 mg/kg, 265 mg/ kg and 350 mg/kg doses to mice as well as scrutinizing the liver histopathology.
Results: Derivatives utilized in experiment had no inhibitory effect on Pseudomonas aeruginosa and Klebsiella pneumoniae, though their inhibitory effect was observed on Staphylococcus aureus and Streptococcus agalactiae. For Staphylococcus aureus and Streptococcus agalactiae the diameters of growth inhibition zone were 8.9-22.3 mm and 16.1-25.6 mm, respectively and MIC of 50-200 and 25-100 µg/ml by order. Additionally, by increasing the injection dose of oxothiazole with 160 mg/ml, 265 mg/ml and 350 mg/ml doses, the hepatitis lesions and liver necrosis were observed in experimental mice.
Discussion: The thiazole derivatives possessed more inhibitory trace on gram positive bacteria than gram negative ones. Furthermore, the likely presence of oxygen link to thiazole ring in tested compounds results in the enhancement of inhibitory potency of these substances. Besides, our results suggest that high doses of oxothiazole cause severe liver damage and rapid death less than 24 hours.
Keywords: Thiazole derivatives, Antibacterial effects, Oxothiazole, Liver toxicity.
Mohammad Mohammaddoost Chakoosari, Mohammad Faezi Ghasemi, Alireza Masiha, Reza Kazemi Darsanaki, Abolfazl Amini,
Volume 9, Issue 5 (Nov,Dec-2015 2015)
Abstract
Abstract
Background and Objective: Probiotics are living microorganisms that have beneficial effects on the health of digestive system. The aim of this study was to evaluate the antimicrobial ability of acidic and neutral supernatants (culture supernatant) of lactic acid bacteria against common bacterial pathogens.
Methods: Four species of lactic acid bacteria (Lactobacillus plantarum PTCC1745, Lactobacillus PTCC1608, Lactobacillus Saki PTCC1712 and Lactobacillus Lactis PTCC1336) were obtained from the microbial collection of Iranian Research Organization for Science and Technology in Lyophilized form. The antimicrobial activity of neutral and acidic supernatants against bacterial pathogens was investigated using the Disk and Well Diffusion Agar methods.
Results: Lactic acid bacteria showed good antimicrobial ability against six pathogenic bacteria with the highest inhibitory effect observed in Lactococcus lactis against E. coli PTCC1399 through well method with an average diameter of 14 mm inhibition zone. In this study, the well diffusion method was far more sensitive compared to the disk method and acidic supernatants showed higher antimicrobial efficiency compared to neutral types.
Conclusion: the Metabolites produced by lactic acid bacteria are able to inhibit the growth of pathogenic bacteria that can be an important and practical solution for the prevention and treatment of infections and ultimately improve human health.
Mohammad Faezi Ghasemi , Seyede Negin Dibadji,
Volume 10, Issue 5 (Sep-Oct-2016 2016)
Abstract
ABSTRACT
Background and Objective: Extended-spectrum beta-lactamases (ESBL) are widely produced by Escherichia coli strains. The aim of this study was to determine frequency of blaOXA-1 and blaSHV genes in E.coli strains isolated from patients hospitalized in city of Rasht, Iran.
Methods: In this cross-sectional study, 200 samples were collected from patients. The E. coli strains were identified using morphological characteristics and biochemical tests. Antimicrobial susceptibility testing was performed. The prevalence of the blaOXA-1 and blaSHV genes in the E. coli isolated was assessed by PCR method.
Results: Overall, 160 E. coli strains were isolated. Of these, 83 (51.9%) showed ESBL activity while 71 (48.1%) did not. All positive strains were resistant to cephalothin. Moreover, 98.8% of ESBL-producing strains were resistance to amoxicillin, cefotaxime and ceftriaxone. The prevalence of the blaOXA-1 and blaSHV genes in ESBL-producing strains were 45% and 17%, respectively. In addition, 28.9% of the strains had both genes while the genes were absent in 9.6% of the strains.
Conclusion: Prevalence of the blaOXA-1gene is higher than that of the blaSHV gene. The absence of both genes in some isolates indicates the possible role of other genes in the ESBL activity.
Keywords: Prevalence, ESBLs, Escherichia coli, blaOXA-1gene, blaSHV gene.
Alireza Ahmadi, Mahdi Ghasemian , Ali Asghar Ayatollahi, Murtadha Al-Khabori, Seyedeh Somayeh Hosseini Alarzi, Mohammad Taher Hojjati,
Volume 15, Issue 5 (Sep-Oct 2021)
Abstract
Background and objectives: Diagnosis glucose intolerance in pregnancy is very important in preventing maternal and fetal complications. In this study, we compared hematological and biochemical characteristics of healthy pregnant women and women with gestational diabetes mellitus (GDM) to find predisposing and prognostic variables of GDM.
Methods: In this study, 80 pregnant women (at 24-28 weeks of pregnancy) were divided into a GDM group and non-GDM group by performing oral glucose tolerance test using 75 g glucose according to the International Association of the Diabetes and Pregnancy Study Groups criteria.
Results: The mean age of women with GDM was significantly higher than those without GDM (p=0.048). Other variables including body mass index, gestational age and daily sleep duration did not differ significantly between the two groups (P>0.05). There was a significant association between family history of diabetes and incidence of GDM (p=0.040). In addition, the C-peptide level was significantly higher in pregnant women with GDM (p=0.004).
Conclusion: Considering the role of C-peptide in predicting metabolic syndrome, it is suggested to use this factor for identification of GDM patients.
