Mj Gharavi, M Roozbehani, Ah Ajodani, S Yosefi Darestani,
Volume 7, Issue 4 (winter[PERSIAN] 2014)
Abstract
Abstract
Background and Objective: The patients with Post-Infectious Irritable bowel syndrome (PI-IBS), a subtype of irritable bowel syndrome, suffer from bacterial gastroenteritis. Since campylobacter Jejuni (CJ) is one of the most common agents in this syndrome, we aimed to evaluate the prevalence of Campylobacter Jejuni in patients with Irritable Bowel Disease.
Material and Methods: This case - control study was conducted on 160 patients divided into 2 equal groups of healthy and unhealthy. The presence of Anti- CJ antibody (IgG and IgA) was evaluated by ELISA and the comparison was performed by chi-square test.
Results: The mean age of case (31.51) and control (31.84) was not statistically different (P = 0.87). Titer of Anti- Campylobacter Jejuni antibody IgG was positive in 25% of patients and 18.8% of the healthy ones (p=0.02). IgA Seropositivity in patients was 7.5% but no one in control group was positive (p =0.01)
Conclusion: Seroprevalence of Cj in patients with IBS was higher significantly than that of control group. Thus, Cj can be known as one of the causes of Post-infection in patients with IBS in our region and it should be paid more attention in diagnostic assessment of these patients.
Keyword: Irritable Bowel Syndrome Campylobacter Jejuni Antibody
Gharavi, Mj, Roozbehani, M, Bakhshkar, H,
Volume 8, Issue 5 (winter[PERSIAN] 2015)
Abstract
Abstract Background and Objectives: Giardia lamblia and Entamoeba histolytica are the most prevalent human intestinal pathogenic protozoa, worldwide. The clinical features of Giardia infection are acute diarrhea, a chronic condition with continuous diarrhea and malabsorption. Entamoeba histolytica invade intestinal tract without any typical clinical indications, and it can involve liver and other organs too. Therefore, we aimed to study these protozoa by serological and parasitological methods. Material and Methods: In this comparative study, the stool and blood specimens were collected from 1025 patients selected via simple random sampling in three different laboratories located in Tehran and Karaj, Iran (2012). Formalin Detergent test was performed on all samples. Both serum and stool positive samples of this method were analyzed for antigen and antibodies related to Giardia lamblia and Entamoeba histolytica, respectively. Results: of 1025 stool specimens, 76 (4.7%) were positive for Giardia lamblia and 19 (1.8%) for Entamoeba histolytica using Formalin-detergent method. In ELISA, 81 (7.9%) coproantibodies to Giardia lamblia and 24 (2.3%) coproantibodies to Entamoeba histolytica, 78 (7.6%) corproantigen for Giardia lamblia, and 5 (0.4%) for Entamoeba histolytica were observed. circulatory antibodies to Entamoeba histlytica were detected in 22 cases (2.1%) Conclusion: Sensitivity of microscopic method compared to serological methods is higher than 90% therefore, Formalin-detergent method can be the best method for stool examination. Key words: Giardia Lamblia and Entamoeba Histolytica, Coproantibody, Coproantigen, Blood Antibody
