Showing 5 results for Fooladi
A Imanifooladi, Mj Soltanpour, R Kachuei, R Mirnejad, M Rahimi,
Volume 2, Issue 1 (Spring - Summer 2008[PERSIAN] 2008)
Abstract
Abstract Background and objectives: Today, specialists who are responsible for controlling infection in hospital use different disinfectants for instrument used in hospital to prevent from transmitting infection. The spectrum of antimicrobial effect, the condition of exploiting, the time of efficiency, the effect on environment, the destructive effect on metal and plastic materials etc. are different therefore, we did this study to determine the antimicrobial effect of five disinfectants on those special resistant bacterial strains. Material and Methods: In the current study the antimicrobial effects of five disinfectant solutions including Nanocide, Anizosin, Sulfanidis, Micros 10, and Colloid on hospital resistant strains by concentrations of 0.5 and 1 McFarland Were examined and evaluated. The concentration of disinfectants was prepared according to proposed protocol by manufacturing company. The time of contact with bacteria was defined in three times (the proposed time by company, less and more than porposed time by company). Results: Nanocide haven't shown any inhibitory effect on resistant strains in none of the three times but Colloid indicates its effect only in more than proposed time. Anizosin in all three times have inhibitory effect on S.aureus in concentration 0.5 and 1 McFarland. Sulfanidis and Micros to have prevented from the growth of resistant strains in all three times. Conclusion: It has been determined that disinfectant Micros 10 made of ammonium chloride stands as first degree of quality and Sulfanidis made of poly hegza methylen bigouanid hydrochloride stands as second degree of quality with respect to proper antibacterial effect for the purpose of disinfecting. Key words: Disinfectant, antiseptic, Staphylococcus auras,Pseudomonas aeroginosa, Acinetobacter calcoaceticus
M Sattari, Aa Imani Fooladi, Gh Godarzi,
Volume 3, Issue 1 (Spring - Summer 2009[PERSIAN] 2009)
Abstract
Abstract Background and objectives: Pseudomonas aeruginosa as an opportunistic pathogen can establish lethal infections in immunocompromised patients or those exposed to predisposing factors. This bacterium contains a single polar flagellum causing motility, chemotaxis and colonization in acute phase of infection. The flagella filament is made up of a structural protein called flagellin. This study was aimed at determining The frequency of fliC gene in Clinical Samples. Material and Methods: In this study, a pair of specific primer for types of flagellin (a, b type) was designed and by using PCR method its structural gene (fliC) was recognized and amplified in clinical strains. Results: This original primer has appropriate efficiency in diagnostic of pseudomonas aeroginosa flagellum. Our study shows that 85% of the Clinical Samples have a fliC gene. Conclusion: This method can be applied to recognizing of the motile strains, and their antigenic typing, and complete amplification of fliC sequence in order to cloning and expression of recombinant flagellin. Key words: Pseudomonas aeruginosa, flagellin, fliC, PCR
Sultanpour M J, Imani Fooladi A A, Bagherpour G, Malek M,
Volume 5, Issue 1 (spring-summer[PERSIAN] 2011)
Abstract
Abstract Background and objectives: Anaerobic microorganisms, particularly bacteroides play an important role in causing Pulp and preapical diseases that lead to chronic abscess. we aimed at studying anaerobie infections in necrotic teeth having Acute and chronic clinical symptoms. Material and Methods: We examined 28 necrotic teeth of 28 patients for the purpose of anaerobic culture fifteen cases with acute clinical symptoms and 13 ones with chronic clinical symptoms. In total 38 root canals from 28 teeth were subjected for examination and sampling. The samples were cultured on thioglycolate medium, sent to laboratory and tested in anaerobic condition for identification of bacteria. Results: The results indicate that 76% of isolated bacteria from necrotic canals obligate anaerobes and 24% are facultative anaerobes. The most common isolated microorganisms are peptostreptococci, fusobacterium and bacteroides. Conclusion: These results are in agreement with the results of other researchers and it has been determined that bacteroids play an important role in destroying the necrotic teeth's bone. Anaerobic bacteria causing dental infactions are less identified and diagnosed, therefore, dentist and clinical lab should take this into account. Key words: Anaerobic Bacteria, Necrotic tooth, Pulp, preapical
T Dadgar, E Ghaemi, N Bahador, A Imani Fooladi, F Kamareie,
Volume 7, Issue 5 (supplement Issue( Bacteriology)[PERSIAN] 2014)
Abstract
Abstract
Background and Objective: The main cause of spreading staphylococcal infections among patients is the healthy carriers working in hospitals. With the secretion of different sorts of toxins such as entrotoxin, this bacteria can provide the conditions for attacking on the host. The main objective of this study is identification of the characteristics and differences in the Staphylococcus aureus isolated from healthy carriers and from the patients on the basis of enterotoxin genes (sea-see).
Material and Methods: One hundred and twenty of the patients and 80 of healthy carriers worked in health centers of Gorgan, north of Iran, were investigated for S. aureus isolate. The isolates were evaluated by PCR for Enterotoxin Genes A-E (SEA to SEE).
Results: Enterotoxin genes (SEA to SEE) was found in 87.5% of the total isolates and the most frequent one was enterotoxin gene sea (N= 124). The prevalence of these isolates in healthy carriers was significantly higher than those of the patients.
Conclusion: Based on the results, the high percentage of S. aureus isolated from clinical samples contains enterotoxin genes. Therefore, Human as the source and carrier of S. aureus is paramount importance, which is due to significant relationship between being toxigenic strains and the source of isolation.
Key words: Staphylococcus Aureus Enterotoxin Patient Carrier
Hosein Soleimanpoor , Mansour Dabirzadeh, Bahman Fooladi ,
Volume 10, Issue 2 (Mar,Apr2016 2016)
Abstract
ABSTRACT
Background and Objective: Chabahar is in Southern Iran located near the Iran-Pakistan border. Since leishmaniasis is an emerging disease in this region, this study aimed to diagnose the disease and identify different species of Leishmania parasite in the patients referred to the central laboratory.
Methods: This descriptive cross-sectional study was conducted in 2011-2012 on patients referred to the central laboratory in the city of Chabahar. The sampling of lesions, slide preparation, culture and PCR specific for kinetoplast DNA (kDNA), extracted from the media and slides, were performed. The data collected by a questionnaire were analyzed by the SPSS software.
Results: The resulted bands from the 48 tested cutaneous leishmaniasis isolates were compared with the standard strains of Leishmania tropica, L. infantum and L. major. All 48 investigated bands were in the 620bp region, which is related to L. major.
Conclusion: Since PCR has high sensitivity and specificity, it is recommended to use kDNA (present in a unique organelle called kinetoplast) for the routine diagnosis and treatment of the disease.
