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Showing 12 results for Esmaeili

J Esmaeili, P Ebrahimy, M Selseleh, P Babadivand,
Volume 6, Issue 2 (Autumn- Winter [PERSIAN] 2012)
Abstract

Abstract Background and objectives: ABO phenotyping is one of the essential tests in Immunohematology. Incompatible blood group transfusion leads to acute hemolysis reactions and other serious complications. Anti A1 is a cold Antibody with no clinical significance, but if it is reacted at 37˚c can be clinically significant, which will be happened rarely. At the present, we report a Case with anti A1 having clinical significance and Anti C. Material and methods: The patient was suffering from Paroxysmal nocturnal hemoglobinuria (PNH) and received repeated blood transfusion. The tests performed for this patient were blood grouping, Antibody screening, panel test and cross match. Result: the patient’s blood group is AB (A2B) with anti A1. The presence of anti C is verified by applying Panel test. Conclusion: to prepare appropriate blood, Cross mach test was performed on A2B blood bags without C Antigen. During laboratory tests and blood transfusion, no reaction was observed. This report indicates that being aware of anti-antigen antibodies is an important point. Key words: Anti C and Anti A1 with Clinical importance, PNH, Ab Screening
R Esmaeili, Ma Amir-Zargar, M Nazari, M Alikhan,
Volume 7, Issue 5 (supplement Issue( Bacteriology)[PERSIAN] 2014)
Abstract

Abstract Background and Objective: Urinary tract infections and bacteremia are the major problems in renal transplant patients, which are mostly due to immunesuppressive regimens, surgery, and exposure to the germs in hospital. The aim of this study was to determine the prevalence of bacterial agents in the blood and urine samples of kidney transplant candidates. Material and Methods: In this one-year-long study, thirty-three renal transplant candidates were assessed for urine and blood cultures. One urine and blood samples from each patient before transplantation and three samples after transplantation were collected. The Samples, using standard microbiological methods, were investigated and infectious organisms identified. Results: In 133 urine samples, Escherichia coli (20.5%), Enterobacter spp. (5.3%), Klebsiella spp. (3 %) and Staphylococcus epidermidis (1.5%) were isolated. In the blood samples, Enterobacter spp. (9.1%), Escherichia coli (6.8%), Staphylococcus epidermidis (3.8%) and Klebsiella spp. (0.8%) were isolated. Conclusion: The results indicate that urinary tract infection was high in patients with transplanted kidney, and E. coli is the most common cause of this infection. Keywords: Kidney Transplantation Bacterial infections Urinary Tract and Blood Infections Escherichia Coli
R Esmaeili, R Yousefi Mashouf, A Moshtaghi, M Alikhani,
Volume 7, Issue 5 (supplement Issue( Bacteriology)[PERSIAN] 2014)
Abstract

Abstract Background and Objective: Bacterial infectioins in particular meningitis, pneumonia and septicemia are still some of the most causes of mortalities in children.The aim of present study was to identify the most common bacterial agents causing infectionis in children under 14 and detection of antibiotic resistance paterns. Material and Methods: During two years,1897samples were obtained from the patients suspected bacterial infectioins. They were investigated for bacterial cultures, age, sex and antibiogram patterns. The species were identified by biochemical and serological methods. Results: Of 1897 samples, 563 (29.6%) had positve bacterial culture. Of these 74.7% were gram negative and 25.3% gram positive . The most common species were Escherichia coli(34.1%), Staphylococcus aureus (17.1%), Psuedomonas aeroginosa (12.4%), Kelebsiella (11%) and Staphylococcus epidermidis (5.7%). The most effective antibiotics against both gram positive and gram negative bacteria were ceftriaoxne, nitrofurantoin, nalidixic acid, amikacin and gentamycin. Conclusion: The gram negative bacteria in particular Escherichia coli, Psuedomonas aeroginosa and Kelebsiella are the predominant causes of bacterial infections in children under 14 in these regions. Most species showed a high relative resisitance to routine antibiotics such as ampicillin, trimethoprim and chloramphenicol. Key Words: Bacteria Infection Children Antibiotic
R Esmaeili, T Hassanzadeh,
Volume 8, Issue 3 (Autumn[PERSIAN] 2014)
Abstract

Abstract Background and Objective: Metabolic syndrome called a cluster of several metabolic disorders is associated with increased risk of cardiovascular diseases. Genetic differences in leptin receptor gene are related with the concentration and activity of leptin in that these discrepancies can influence lipid levels. We aimed to determine the association between the leptin receptor gene polymorphism on serum lipid profile and leptin activity in metabolic syndrome patients. Material and Methods: This case-control study was conducted on 200 patients with metabolic syndrome and 200 healthy individuals. Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphisms (RFLP) were used to determine genotypic distribution and allelic frequencies of polymorphisms, respectively. The plasma leptin activity was measured by a kit in a fluorescence spectrometer, and Lipid concentration by routine biochemical and enzymatic assays. Results: Two groups had significant differences in all measured factors such as lipid profiles, fast blood sugar, waist circumference, blood pressure and leptin concentration (P< 0.05). Conclusion: Given that the two groups had significant differences in blood and body measurements, no role of K656N polymorphism was observed. Overall, Lys656Asn (K656N) polymorphism of leptin receptor gene is not associated with serum lipid profile and leptin activity with metabolic syndrome. Keywords: Metabolic Syndrome, Leptin Receptor Gene, PolymorphismK656N
Azad Khaledi , Daymad Fatemeh , Seyyed Mohammad Javad Hosseini , Maryam Meskini , Davoud Esmaeili ,
Volume 12, Issue 6 (Nov - Dec 2018)
Abstract

ABSTRACT
            Background and Objectives: Acinetobacter baumannii is an opportunistic pathogen that affects different groups of people, especially intensive care unit (ICU) patients. The prevalence of infections caused by this bacterium is very high. Today, prevalence of infections caused by multidrug-resistant (MDR) and extreme-drug resistant (XDR) strains is increasing. This study aimed to determine the antibiotic susceptibility pattern of A. baumannii isolates from ICU patients.
            Methods: This cross-sectional study was conducted from October 2014 to March 2015 on patients admitted to ICU of Imam Khomeini hospital in Tehran, Iran. Clinical samples of various sources were collected from patients. Isolates were detected and identified via microbiological and biochemical tests as well as PCR amplification of the blaOxa51 gene. Then, susceptibility testing was performed using the Kirby-Bauer disk diffusion test. Statistical analysis was performed with SPSS (version 22, Chicago, IL, USA) using Chi-square and Fisher’s exact tests.
            Results: Of the total of 62 clinical samples, 24 (39%) were respiratory samples and only three (6%) were cerebrospinal fluid samples. Most MDR and XDR strains were isolated from respiratory samples. The highest resistance rate was against ceftriaxone, ticarcillin and erythromycin (100%), while the lowest resistance rate was against to minocycline (20%).
            Conclusion: Owing to detection of high multi-drug resistance isolates in the present study, and importance of multi-drug resistance in A. baumannii, the identification of multi-drug resistance genes and their reporting to health care/treatment centers is important. Thus, it is recommended to perform susceptibility testing to help determine the most effective antibiotic(s) for the treatment of infections in ICU patients.
            Keywords:  Acinetobacter baumannii, MDR, XDR, ICU.

Maryam Meskini , Azad Khaledi , Davoud Esmaeili ,
Volume 13, Issue 1 (Jan-Feb 2019)
Abstract

ABSTRACT
            Background and Objectives: Pseudomonas aeruginosa is a gram negative opportunistic pathogen and an important cause of wound infections and nosocomial infections. The purpose of this study was to study inhibitory effects of a new ointment prepared from medicinal plants against P. aeruginosa isolates.
            Methods: In this study, an ointment called ZOUSH was prepared from mixing alcoholic extracts of Satureja khuzestaniea, Zataria multiflora, Mentha mozaffariani Jamzad, honey and polyurethane. Minimal inhibitory concentration of ZOUSH and its compositions alone or combined was determined using the disk diffusion method.
            Results: S. khuzestaniea, Z. multiflora and Mentha mozaffariani Jamzad had inhibitory effects against P. aeruginosa. The ZOUSH ointment had greater antibacterial effects than the any of its compositions used solely or combined. The diameter of inhibition zone had a direct relationship with the concentration of the extracts. Moreover, the antibacterial effect of the ZOUSH ointment was identical to that of polymyxine B (300 µg).
            Conclusion: We demonstrated that the ZOUSH ointment has inhibitory effects against P. aerugionosa. The inhibition zone diameter is directly correlated with the concentration of the extracts. Our results suggest that the ointment could be useful for treatment of burn wounds and skin infections.
Hoorieh Hoseinpoor , Davoud Esmaeili ,
Volume 13, Issue 3 (May-Jun 2019)
Abstract

ABSTRACT
             Background and Objectives: In recent years, infections caused by antibiotic-resistant strains of Staphylococcus aureus have become a major health issue. It has been suggested that the extract or essential oil of cinnamon tree has antibacterial properties. In this study, we investigated the antibacterial effects of polyurethane-cinnamon nanofibers against clinical isolates of S. aureus.
             Methods: Polyurethane-Cinnamomum zeylanicum nanofiber scaffolds were synthesized. Polyurethane nanofiber and cinnamon polymers were also used to prepare the scaffold under the electrospinning process. Infrared spectroscopy, electron microscopy and mechanical tensile test were utilized to assess the scaffolds. Minimum inhibitory concentration of the nanofiber against the bacteria was determined using the broth dilution method according to the Clinical & Laboratory Standards Institute guidelines.
Results: The results of antibiogram test showed that all tested disks were susceptible to S. aureus. Diameter of growth inhibition zone for polyurethane-cinnamon 10% was 41 mm. Minimum inhibitory concentration of the nanofiber against S. aureus isolates was 0.02 μg/mL.
             Conclusion: We demonstrated that the polyurethane-cinnamon nanofiber has favorable antibacterial effects against clinical isolates of S. aureus. It is recommended to conduct further studies on the antibacterial effects of this nanofiber on other bacteria.
             Keywords: Staphylococcus aureus, Cinnamon, Nano fiber.

Maryam Meskini, Mohsen Korani , Davoud Esmaeili ,
Volume 13, Issue 4 (Jul-Aug 2019)
Abstract

Background and Objectives: Burns are a major global public health problem, accounting for an estimated 180,000 deaths annually. The majority of burn-related deaths occur in low- and middle-income countries. Considering the importance of treating infected burn wounds with the least adverse effects, we aimed to search the literature to find new treatments for infected wounds using medicinal plants.
Methods: The search process was carried out using various databases including Google Scholar, ScienceDirect, Web of Science, MEDLINE, PubMed, Scopus, and the Cochrane Library. We searched for relevant original and review articles (published in English or Persian) using the following keywords: herbal extract, herbal medicine, burn infection, and wound infection.
Results: Overall, we found approximately 100 articles related to the use of medicinal plants for treatment of wounds or infections. According to these studies, main constituents of plant extracts were carvacrol, flavonoids, terpenoids, phenolic diterpenes, and phenolic acids. Most studies assessed the antimicrobial activity by determining minimum inhibitory concentration and minimum bactericidal concentration using the disc diffusion method.
Conclusion: Given the favorable antimicrobial activity of medicinal plants, it is recommended to use them for treatment of burn wound infections.
 
Hamidreza Ebrahimnezhad, Leila Barzegar, Davoud Esmaeili,
Volume 14, Issue 1 (Jan-Feb 2020)
Abstract

ABSTRACT
           Background and Objectives: Probiotics are live microorganisms that function through  various mechanisms  and affect the alteration of the commensal microbiota against pathogens. Nowadays,  given the problems associated with antibiotics use, probiotic strains  offer a novel and  appropriate  alternative for the treatment of diseases such as diarrhea. The aim of this study was to investigate the antibacterial synergism of Lactobacillus spp., Bifidobacterium spp. and Escherichia coli strain Nissle 1917 (ECN) on the clinical sample of diarrheagenic E.coli and Campylobacter jejuni.
           Methods: A paper disk-diffusion technique was used to evaluate the antibacterial activity. Sterile 6 mm paper disks were saturated with probiotic suspensions made by settling probiotic medications into distilled water. Three kinds of disk were prepared. One disk was prepared for Lactobacillus spp. and Bifidobacterium spp., another for ECN, and the third was made by combined probiotics. Clinical samples of diarrheagenic E.coli and Campylobacter jejuni were cultivated on Muller Hinton agars, and disks were placed on the inoculated Muller Hinton agars. All plates were incubated under microaerophilic and appropriate conditions.
           Results: The zone of inhibition (ZOI) of the bacterial growth was measured. All pathogenic microorganisms showed sensitivity to the probiotic disks. The combined disks had better effects against pathogens compared with single disks.
           Conclusion: A considerable synergistic effect was observed in the results of combined probiotics; therefore, combined strains can be more efficient against intestinal pathogens in comparison with single probiotics.
           Keywords: Probiotic, Lactobacillus, Bifidobacterium, Escherichia coli Nissle, Diarrhea, Campylobacter jejun.i.

Neda Gharagozloo Hesari, Davoud Esmaeili, Taher Mohammadian, Mohammad Hasan Shahhosseini, Atousa Ferdosi,
Volume 14, Issue 6 (Nov-Dec 2020)
Abstract

Background and objectives: Industrial wastewater is worldwide health concern. Microorganisms present in the environment have an important role in the biodegradation of lipids, fats and proteins from wastewater. In this regard, microbial lipases and proteases are interesting research targets because of high stability, broad substrate specificity, high yields and availability. In this study, we analyze sequences encoding lipase of Pseudomonas putida and subtilisin of Bacillus subtilis for generation of a new recombinant protein for degradation of environmental contaminations caused by lipids and proteins.
      Methods: In this study, sequences of the genes encoding lipase and subtilisin were obtained from GenBank. To predict the 3D structure of the protein, modeling was carried out. The prediction of secondary structure, tertiary structure and solvent accessibility was carried using bioinformatics tools including I-TASSER, GoR4 and ExPasy.
      Results: The lipase-subtilisin fusion protein was well-characterized by bioinformatical studies with appropriate spatial and secondary structures. The protein had appropriate hydrophilicity, biological half-life and thermal and acidic stability. The codon optimization was performed appropriately.
      Conclusion: Overall, the bioinformatical analysis of the designed protein showed that the recombinant lipase-subtilisin protein has a stable structure both in vitro and in vivo, a negative normalized B-factor and lipolytic and proteolytic activities, which makes it suitable for treatment of lipid and protein contaminations.
Dr Mohsen Ebrahimi, Dr Hassan Esmaeili,
Volume 15, Issue 6 (Special issue (Nov-Dec) 2021)
Abstract

Infective endocarditis is rare in children but can cause significant morbidity and mortality. Streptococcus and Staphylococcus species are the leading causes of this disease. Staphylococcus is more common in people with underlying heart disease, and Streptococcus viridans is more common in people who have had a dental procedure. In general, any fever of unknown origin in children with an underlying heart problem should be carefully evaluated for endocarditis, and empiric therapy should be performed. The main symptoms of the disease include fever, new murmur, deterioration of the previous murmur, hematuria, embolic events, splenomegaly, bleeding splinter, Osler's nodes, Janeway lesion, and Roth spots. One of the important complications of infective endocarditis is cerebrovascular event and stroke. Herein, we describe a 6-year-old girl presented with fever and skin lesions and no history of underlying heart problem or dental procedure. The patient expired after three days of mitral valve infection with S. aureus.
Hassan Esmaeili, Samaneh Shams Nateri, Mohsen Ebrahimi,
Volume 17, Issue 6 (Nov-Dec 2023)
Abstract

Background: Kawasaki disease (KD) is a febrile systemic vasculitis that affects children. Further research is required due to the disease's significance, consequences, and increasing prevalence. This study aimed to determine the frequency of coronary artery complications in patients with KD referred to Taleghani Hospital (Gorgan, Iran) from 2007 to 2017.
Methods: Between 2007 and 2017, 108 cases with a definitive diagnosis of KD were studied retrospectively. Data were obtained from medical records and the patients' biographical, clinical, laboratory, radiographic, and echocardiographic data.
Results: A total of 108 patients diagnosed with KD were involved in the study, 21 (19.44%) of whom showed coronary artery-associated complications. There was no significant difference in clinical and laboratory findings and coronary artery involvement between male and female groups (P-value < 0.05). Left coronary artery (LCA) involvement and the pattern of vascular involvement as ectasia and aneurysm were significantly higher in the echocardiography of the studied patients (P-value = 0.0001).
Conclusion: Kawasaki disease-related coronary artery complication is more common in children under the age of 3, particularly males, in Gorgan. The LCA is the most common site of coronary artery complications in KD children. The findings can improve the prognosis of KD patients and their related heart diseases.




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