This paper should be cited as: Roohi, Z. Khaki, P. Moradi Bidhendi, S.
Distribution of Anti-leptospira Antibodies in the Sera of Patients Suspected
Leptospirosis
Roohi, Z. (MSc)1, Khaki, P. (PhD)2, Moradi Bidhendi, S. (PhD)3
1. MSc
of Microbiology, Leptospira Laboratory Reference, Razi Vaccine & Serum Research Institute, hessarak, Karaj, Iran
2. Assistant
Professor of Microbiology, Leptospira Laboratory
Reference, Razi Vaccine & Serum Research
Institute, Karaj, Iran
3. Assistant
Professor of Microbiology, Leptospira Laboratory
Reference, Razi Vaccine & Serum Research
Institute, Karaj, Iran
Abstract
Background
and Objective: Leptospirosis is a zoonosis infectious disease that is prevalent in tropical and
subtropical regions and is caused by the pathogenic serovars
of leptospires. Hence, we aimed at investigating the
prevalence of antibodies against these bacteria in the blood samples of
suspected leptospirosis.
Material
and Methods: the human serum samples (N = 130) were
obtained from patients clinically suspected leptospirosis. The Serum level of IgM antibodies were studied by ELISA kit (PrioCHECK) in Razi Vaccine and
Serum Research Institute (Karaj), 2011-2012.
Results:
Anti-leptospira IgM class was observed in 21(16%) samples. The relative
distribution of the disease was reported in men (80.95%), women (19.04%), and
farmers (30.95%) and in 20-40-year group (57.14%).Contact with contaminated
water was the most common cause of infection (52.38%) and fever was the most
common sign of Leptospirosis (72.2%).
Conclusion: Due to the occurrence
of anti-leptospira antibodies in 16% of
suspected cases, it is recommended that routine ELISA be done at least in major
diagnostic centers.
Keywords: Leptospira, Leptospirosis, Human,
ELISA
Corresponding Author: Moradi
Bidhendi, S.
Email: s.bidhendi@rvsri.ac.ir
Received: 30 Oct 2013
Revised: 2 Apr 2014
Accepted: 5 Apr 2014