Volume 9, Issue 4 (sep,Oct 2015 2015)                   mljgoums 2015, 9(4): 15-21 | Back to browse issues page


XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ebrahim Z, Tadayon K, Mosavari N. Genomic Characterization of the Vaccinal Strain of Mycobacterium Avium Subspecies Paratuberculosis (MAP) 316F by MIRU-VNTR. mljgoums. 2015; 9 (4) :15-21
URL: http://goums.ac.ir/mljgoums/article-1-773-en.html
1- Razi Vaccine & Serum Research Institute, Karaj Iran
2- Razi Vaccine & Serum Research Institute, Karaj, Iran
Abstract:   (3979 Views)

Abstract

       Background and Objective: Paratuberculosis is a chronic granulomatous enteritis of ruminants caused by Mycobacterium avium subspecies paratuberculosis (MAP). this study aimed to characterize the genome of the MAP 316F strain.

      Methods: The MAP 316F strain was subjected to the PCR-F57 and PCR-IS900 experiments in order to ensure its identity as MAP. This was followed by application of the Thibault genotyping system consisting of eight loci including 292, x3, 25, 47, 3, 7, 10 and 32. Required genomic material for all experiments was prepared using the simple method of boiling. Gel electrophoresis findings related to the typing PCRs were backed by sequencing of amplification products.

      Results: In PCR amplification, eight products with the size of 300, 298, 350, 217, 208, 203, 803 and 649 bp were detected at 292, X3, 25, 47, 3, 7, 10 and 32 loci, holding 3, 2, 3, 3, 2, 2, 2 and 8 copies of TRs at these loci, respectively.

      Conclusion: This genomic pattern is matched with that of the MAP 316F vaccine strain from the French Merial company and also the MAP K10 fully-sequenced strain.

       Keywords: Mycobacterium avium subsp. paratuberculosis, Genomics, Genotyping techniques, Strain

Full-Text [PDF 510 kb]   (1091 Downloads)    
Type of Study: Original Paper | Subject: Special
Received: 2015/11/25 | Accepted: 2015/11/25 | Published: 2015/11/25

Add your comments about this article : Your username or Email:
CAPTCHA code

© 2007 All Rights Reserved | Medical Laboratory Journal