Volume 9, Issue 4 (sep,Oct 2015 2015)                   mljgoums 2015, 9(4): 1-5 | Back to browse issues page


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Ebrahimzadeh A, Davoodi T, Pashaei Naghadeh A. Genetic Diversity Block 2 of Surface Protein-1 in Plasmodium Falciparum Merozoite by Nested-PCR Method in Southeastern Iran. mljgoums. 2015; 9 (4) :1-5
URL: http://mlj.goums.ac.ir/article-1-771-en.html
1- Research Center for Infectious and Tropical Diseases, Zahedan University of Medical Sciences, Iran
2- Zahedan University of Medical Sciences, Iran , tahereh2015@yahoo.com
3- Zabol University of Medical Sciences, Iran
Abstract:   (6654 Views)

Abstract
      Background and Objectives: Plasmodium falciparum merozoite surface protein-1 (PfMSP-1) is a promising vaccine against malaria during its blood stages which play an important role in immunity to this disease. Polymorphic nature of this gene is a major obstacle in making an effective vaccine against malaria. In this study, the genetic diversity of Plasmodium falciparum isolates was investigated in Sistan-Baluchestan Province using allelic families of the MSP-1.
       Methods: From March/April 2011 to August/September 2012, 94 blood samples were collected from patients with falciparum malaria who were living in four districts of Sistan-Baluchestan Province. The extracted genomic DNA and genetic diversity of MSP-1 block 2 were evaluated by nested polymerase chain reaction.
        Results: From a total of 94 patients, 89 patients (94.7%) had positive PCR results and the remaining five patients were excluded. Seven different alleles of MSP-1 were identified through size difference on agarose gel. Comprising 46.1% of the samples, MAD20 was identified as the predominant MSP-1 allelic family, while the RO33 family had the lowest frequency (with 7.9%). In 10% of samples infection with two alleles was observed.
         Conclusion: The results of this study suggest that genetic diversity of PfMSP-1 in Southeastern Iran is relatively low and most infections originate from a clone that is consistent with an area of low malaria transmission. This information is useful for the prevention and control of malaria in Iran.
          Keywords: Merozoite Surface Protein 1, Plasmodium Falciparum, Polymerase Chain Reaction
 

Full-Text [PDF 577 kb]   (1156 Downloads)    
Type of Study: Original Paper |
Received: 2015/11/25 | Accepted: 2015/11/25 | Published: 2015/11/25 | ePublished: 2015/11/25

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