Volume 9, Issue 3 (Jul,Aug2015[PERSIAN] 2015)                   mljgoums 2015, 9(3): 83-88 | Back to browse issues page

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shakerian, A, Sharafati-chaleshtori, R, Karshenas, AA, Rahimi, E. Molecular Detection of Cryptosporidium Parvum in Cow’s Raw Milk in Isfahan Province, 2013. mljgoums 2015; 9 (3) :83-88
URL: http://mlj.goums.ac.ir/article-1-746-en.html
1- School of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
2- Kashan University of Medical Sciences, Kashan, Iran
3- Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
4- Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
Abstract:   (12340 Views)

Abstract

Background and Objective: Cryptosporidium parvum is a zoonotic protozoan parasite causing diarrheal cryptosporidiosis. Numerous outbreaks of cryptosporidiosis have been reported worldwide.  The transmission via milk, water and raw animal products is one of the important ways. The aim of this study was the identification of hsp70 gene in Cryptosporidium parvum in raw cow’s milk samples.

Material and Methods: In this cross sectional study, 38 raw cow’s milk samples of bulk tank were randomly collected from traditional and semi industrial cattle farms in Isfahan.  To identify the protozoa in milk samples, the extracted DNA was evaluated by Nested polymerase chain reaction (PCR).

Results: Based on Nested polymerase chain reaction, 2 samples (5.26%) were infected to Cryptosporidium parvum.

Conclusions: The contamination of milk with Cryptosporidium Parvum is less than that of the other foodstuffs. Thus, it is necessary to reduce food contamination and to have appropriate health education programs.

Keywords: Cryptosporidium Parvum, Milk; Polymerase Chain Reaction.

Research Article: Original Paper |
Received: 2015/10/13 | Accepted: 2015/10/13 | Published: 2015/10/13 | ePublished: 2015/10/13

Add your comments about this article : Your username or Email:
CAPTCHA

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2007 All Rights Reserved | Medical Laboratory Journal

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.