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:: Volume 4, Number 2 (Autumn – Winter 2011[PERSIAN] 2010) ::
mljgoums 2010, 4(2): 31-37 Back to browse issues page
The Detection of Fimbrial Pathogenic Genes in E. coli Strains Isolated from Patients with Urinary Tract Infection
A Nazemi *1, M Naderi2, M Jafarpour3, M Mirinargesi3, SH Sharifi4
1- (phD) Assistant professor, Department of Biology, , alinazemy@yahoo.com
2- ( BSc )
3- (phD)
4- (MSc)
Abstract:   (9788 Views)
Abstract Bachground and objectives: The ability of adherence to the surface of host cell is very critical in the colonization of microbial pathogens. It has been revealed that E. coli strains that infect urinary tracts have different fimbrea such as I, S, FIC, Dr, and fimbrial adhesions. Material and Methods: In this study, 363 urine samples were obtained from patients with urinary tract infections reffered to clinical laboratories in Western areas of Tehran ,2008-2010 by using biochemical tests,200 samples were confirmed to be E.coli.First, DNA was extracted by boiling method and then the presence of fimbria fim, sfa, pap, foc, and afa genes tested by PCR. Results: In 200 samples, the frequency of fimbria fim, sfa, pap, foc, and afa genes are188 (%94 ), 34 (%17), 20 (%10), 61 (%31) and 71 (%35.5), respectively. Conclusion: The resultes show that FIM ans SFA are the most fimbrial genes of E. coli isolated from urine samples .This information can be valuable in etiology of urinary tract infection (UTI), UTI administration, and making of new vaccines. Key words: Urinary tract infection, fimbria, Uropathogenic E. coli (UPEC)
Keywords: Key words: Urinary tract infection, fimbria, Uropathogenic E. coli (UPEC)
Full-Text [PDF 22 kb]   (3867 Downloads)    
Type of Study: Research | Subject: Special
Received: 2011/10/9 | Accepted: 2013/06/8 | Published: 2013/06/8
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Nazemi A, Naderi M, Jafarpour M, Mirinargesi M, Sharifi S. The Detection of Fimbrial Pathogenic Genes in E. coli Strains Isolated from Patients with Urinary Tract Infection. mljgoums. 2010; 4 (2) :31-37
URL: http://goums.ac.ir/mljgoums/article-1-90-en.html
Volume 4, Number 2 (Autumn – Winter 2011[PERSIAN] 2010) Back to browse issues page
مجله علوم آزمایشگاهی دانشکده پیراپزشکی گرگان Medical Laboratory Journal
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