1- MSc Student Research Committee, Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran, Student Research Committee, Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran 2- MD Department of Hemodialysis, 5th Azar Hospital, Gorga, Iran, Department of Hemodialysis, 5th Azar Hospital, Gorga, Iran 3- BSc Student Research Committee, Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran, Student Research Committee, Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran 4- PhD Infectious Diseases Research Center, Department of Microbiology، Golestan University of Medical Sciences, Gorgan, Iran, Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Abstract: (627 Views)
ABSTRACT Background and Objectives: Human cytomegalovirus (HCMV) is the most common viral cause of morbidity and mortality in immunocompromised patients. The aim of this study was to evaluate the frequency of active CMV infection in hemodialysis patients in Gorgan, Iran. Methods: Plasma samples were obtained from 149 hemodialysis patients at Hemodialysis Unit of Panje-Azar Medical Centre in Gorgan, Iran. Presence of CMV-DNA in plasma samples was evaluated by polymerase chain reaction (PCR) using specific primers for highly conserved regions of major capsid protein gene of HCMV. In addition, level of CMV-IgM antibody was measured by serological testing. Demographic information and past medical history of patients were also recorded. Data was analyzed by SPSS software (version 18). Results: Total prevalence of CMV infection was 6.7% (10/149) among the patients receiving hemodialysis. CMV-DNA and anti-CMV IgM antibody were detected in 2.68% and 4.69%, of the samples, respectively. One case was found positive for both CMV-DNA and anti-CMV IgM antibody. CMV infection did not have any correlation with gender, age, ethnicity, duration of hemodialysis, and history of blood transfusion. Conclusion: A notable proportion of hemodialysis patients in Gorgan have active CMV infection. Accurate detection of these individuals is important for preventing infection spread, especially in immunocompromised individuals. Simultaneous diagnosis of CMV infection using serological testing and PCR assay could help reduce the risk of infection spread. Keywords: HCMV, Hemodialysis, PCR, Iran.
Type of Study: Original Paper |
Subject: Special Received: 2017/08/8 | Accepted: 2017/08/8 | Published: 2017/08/8
1. Enan KA, Rennert H, El-Eragi AM, El Hussein A, Elkhidir IM. Comparison of Real-time PCR to ELISA for the detection of human cytomegalovirus infection in renal transplant patients in the Sudan. Virol J. 2011; 8(1): 222. doi:10.1186/1743-422X-8-222. [DOI:10.1186/1743-422X-8-222]
2. Sepehrvand N, Khameneh Z, Eslamloo H. Survey the seroprevalence of CMV among hemodialysis patients in Urmia, Iran. Saudi Journal of Kidney Diseases and Transplantation. 2010; 21(2): 363.
3. Sia IG, Patel R. New strategies for prevention and therapy of cytomegalovirus infection and disease in solid-organ transplant recipients. Clinical microbiology reviews. 2000;13(1): 83-121. [DOI:10.1128/CMR.13.1.83-121.2000]
4. Ocak S, Duran N, Eskiocak AF. Seroprevalence of cytomegalovirus antibodies in haemodialysis patients. Turkish Journal of Medical Sciences. 2006; 36(3):155-158.
5. Abou-El-Yazed E, El-Hoseny IM, Kasim K, El-Sadek AA, Morsy, Amar A. Prevalence of cytomegalovirus infection among patients undergoing hemodialysis. Egyptian Journal of Immunology. 2008; 15(2): 33-41.
6. Pescovitz M. Review of the CMV in renal transplantation. Saudi Journal of Kidney Diseases and Transplantation. 2007;18(4):505.
7. Grassi M, Mammarella A, Sagliaschi G, Granati L, Musca A, Traditi F, et al. Persistent hepatitis G virus (HGV) infection in chronic hemodialysis patients and non-B, non-C chronic hepatitis. Clinical chemistry and laboratory medicine. 2001; 39(10): 956-60. [DOI:10.1515/CCLM.2001.154]
8. Ross S, Novak Z, Pati S, Boppana S. Diagnosis of Cytomegalovirus Infections. Infectious disorders drug targets. 2011; 11(5): 466-474. [DOI:10.2174/187152611797636703]
9. Saygun I, Yapar M, Özdemir A, Kubar A, Slots J. Human cytomegalovirus and Epstein-Barr virus type 1 in periodontal abscesses. Oral microbiology and immunology. 2004; 19(2): 83-7. [DOI:10.1046/j.0902-0055.2002.00118.x]
10. Mencarelli F, Marks SD. Non-viral infections in children after renal transplantation. Pediatric Nephrology. 2012;27(9):1465-76. [DOI:10.1007/s00467-011-2099-z]
11. Kelishadi M, Mojerloo M, Hashemi P, Tabarraei A. Routine Screening for Cytomegalovirus Infection in Immunosuppressed Patients: Can the Diagnostic Tests Alone Be Reliable? Jundishapur J Chronic Dis Care. 2016; 5(1): e32041. [DOI:10.17795/jjcdc-32041]
12. Revello MG, Gerna G. Diagnosis and management of human cytomegalovirus infection in the mother, fetus, and newborn infant. Clinical microbiology reviews. 2002; 15(4): 680-715. [DOI:10.1128/CMR.15.4.680-715.2002]
13. Nafar M, Roshan A, Pour-Reza-Gholi F, Samadian F, Ahmadpoor P, Samavat S, et al. Prevalence and risk factors of recurrent cytomegalovirus infection in kidney transplant recipients. Iranian journal of kidney diseases. 2014; 8(3): 231-5. رفرنس های متنی مثل خروجی کراس رف را در اینجا وارد کرده و تایید کنید