1- PhD Department of Veterinary Basic Science, College of Veterinary Science, Science and Research branch, Islamic Azad University, Tehran, Iran, Department of Veterinary Basic Science, College of Veterinary Science, Science and Research branch, Islamic Azad University, Tehran, Iran 2- PhD Golestan Research Center of Gastroenterology and Hepatology-GRCGH, Golestan University of Medical Sciences, Gorgan, Iran, Golestan Research Center of Gastroenterology and Hepatology-GRCGH, Golestan University of Medical Sciences, Gorgan, Iran 3- PhD Department of Botany, Gorgan Branch, Islamic Azad University, Gorgan, Iran, Department of Botany, Gorgan Branch, Islamic Azad University, Gorgan, Iran 4- PhD Golestan Research Center of Gastroenterology and Hepatology-GRCGH, Golestan University of Medical Sciences, Gorgan, Iran, Golestan Research Center of Gastroenterology and Hepatology-GRCGH, Golestan University of Medical Sciences, Gorgan, Iran , firstname.lastname@example.org
Abstract: (541 Views)
ABSTRACT Background and Objectives: Capparis spinosa has been used in traditional medicine for various applications including treatment of liver disorders and cancer. We studied the effects of this plant on cell proliferation and morphological characteristics of PLC/PRF/5 liver cancer cell line. Methods: After preparing ethanolic extract of the plant, the inhibitory effect of the extract was assessed using MTT assay, and morphological changes were assessed by an inverted microscope. Results: C. spinosa ethanolic extract exhibited anti-cancer effects in a concentration-dependent manner. Half-maximal inhibitory concentration of the extract was 1051±4.21 mg/mL. Morphological changes including cell shrinkage, reduction of cell volume and nuclear condensation confirmed the inhibitory effect of C. spinosa on PLC/PRF/5 cells. Conclusion: According to the results of this study, extract of C. spinosa seems to be suitable for prevention and treatment of liver cancer. Further studies on animal models could verify the efficiency of the extract against cancer cells.
Type of Study: Original Paper |
Subject: Special Received: 2017/08/8 | Accepted: 2017/08/8 | Published: 2017/08/8
1. Llovet JM, Ricci S, Mazzaferro V, Hilgard P, Gane E, Blanc JF, et al. Sorafenib in advanced hepatocellular carcinoma. The New England journal of medicine. 2008; 359(4): 378-90. DOI: 10.1056/NEJMoa0708857. [DOI:10.1056/NEJMoa0708857]
2. El-Serag HB. Hepatocellular carcinoma. The New England journal of medicine. 2011; 365(12): 1118-27. DOI: 10.1056/NEJMra1001683. [DOI:10.1056/NEJMra1001683]
3. Marrero JA, Feng Z, Wang Y, Nguyen MH, Befeler AS, Roberts LR, et al. Alpha-fetoprotein, des-gamma carboxyprothrombin, and lectin-bound alpha-fetoprotein in early hepatocellular carcinoma. Gastroenterology. 2009; 137(1): 110-8. doi: 10.1053/j.gastro.2009.04.005. [DOI:10.1053/j.gastro.2009.04.005]
4. McMichael AJ. Food, nutrition, physical activity and cancer prevention. Authoritative report from World Cancer Research Fund provides global update. Public health nutrition. 2008; 11(7): 762-3. [DOI:10.1017/S1368980008002358]
5. Newman DJ, Cragg GM. Natural products as sources of new drugs over the 30 years from 1981 to 2010. Journal of natural products. 2012; 75(3): 311-35. doi: 10.1021/np200906s. [DOI:10.1021/np200906s]
6. Eddouks M, Lemhadri A, Michel JB. Caraway and caper: potential anti-hyperglycaemic plants in diabetic rats. Journal of ethnopharmacology. 2004; 94(1): 143-8. DOI: 10.1016/j.jep.2004.05.006. [DOI:10.1016/j.jep.2004.05.006]
7. Jiang HE, Li X, Ferguson DK, Wang YF, Liu CJ, Li CS. The discovery of Capparis spinosa L. (Capparidaceae) in the Yanghai Tombs (2800 years b.p.), NW China, and its medicinal implications. Journal of ethnopharmacology. 2007; 113(3): 409-20. [DOI:10.1016/j.jep.2007.06.020]
8. Kulisic-Bilusic T, Schmoller I, Schnabele K, Siracusa L, Ruberto G. The anticarcinogenic potential of essential oil and aqueous infusion from caper (Capparis spinosa L.). Food chemistry. 2012; 132(1): 261-7. doi: 10.1016/j.foodchem.2011.10.074. [DOI:10.1016/j.foodchem.2011.10.074]
9. Eddouks M, Lemhadri A, Michel JB. Hypolipidemic activity of aqueous extract of Capparis spinosa L. in normal and diabetic rats. Journal of ethnopharmacology. 2005; 98(3): 345-50. [DOI:10.1016/j.jep.2005.01.053]
10. Panico AM, Cardile V, Garufi F, Puglia C, Bonina F, Ronsisvalle G. Protective effect of Capparis spinosa on chondrocytes. Life sciences. 2005; 77(20): 2479-88. [DOI:10.1016/j.lfs.2004.12.051]
11. Tabor E. Hepatocellular carcinoma: global epidemiology. Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver. 2001; 33(2): 115-7. [DOI:10.1016/S1590-8658(01)80062-1]
12. Srivatanakul P, Sriplung H, Deerasamee S. Epidemiology of liver cancer: an overview. Asian Pacific journal of cancer prevention. APJCP. 2004; 5(2): 118-25.
13. Lam SK, Ng TB. A protein with antiproliferative, antifungal and HIV-1 reverse transcriptase inhibitory activities from caper (Capparis spinosa) seeds. Phytomedicine. international journal of phytotherapy and phytopharmacology. 2009; 16(5): 444-50. doi: 10.1016/j.phymed.2008.09.006. [DOI:10.1016/j.phymed.2008.09.006]
14. Aghel N, Mombeini A. Hepatoprotective activity of Capparis spinosa root bark against ccl4 induced hepatic damage in mice. Iranian journal of pharmaceutical research : IJPR. 2007; 6(4): 285-290.
15. Sheikh M, Eshraghi HR, Khoshnia M, Mazandarani M, Moradi A. Cytotoxicity effect of Capparis spinosa L. on the HepG2 human hepatocellular carcinoma cell line. Indian J Tradit Knowle. 2017; 6(1): 230-8. رفرنس های متنی مثل خروجی کراس رف را در اینجا وارد کرده و تایید کنید