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:: Volume 2, Number 2 (Autumn – Winter 2009[PERSIAN] 2008) ::
mljgoums 2008, 2(2): 0-0 Back to browse issues page
Prevalence of Campylobacter jejuni in Diarrhea samples in Gorgan ,East north of Iran
N Ziaei *1, N Amir Mozafari2, H Kouhsari3, A Moradi4, A Tabarai5, T Dadgar4, S Livani6, M Arab Ahmadi6
1- (MSC) Ms in Microbiology,Lahijan Azad , neda.ziaee61@gmail.com
2- (phD) Associate professor in Molecular Biology,
3- (MID)
4- MSC
5- (phD)
6- ( BSc )
Abstract:   (18122 Views)
Abstract Background and Objectives: Diarrhea is one of the most common diseases in the world. Campylobacter jejuni is one of the prevalent agents of bacterial diarrhea in most of developing countries. It is usually ignored in routine laboratory test in our country, because it has a difficult investigation method. This article aims to determine the prevalence of Campylobacter jejuni, in diarrhea samples in Gorgan City (East north of Iran) by PCR Method. Material and Methods: This descriptive study was performed on 455 diarrheal samples during one year (2005-06).255 out of them were cultured on Preston media (Himedia co.) on 42°c. DNA Extracted by phenol cholorophorm method was directly carried out on stool samples.16srDNA hipo and asp primers for detection of Campylobacter genus, C.jejuni and C.coli species were used, respectively. In addition, universal primer of 16srDNA was used for control of PCR method. Results: no sample was positive for Campylobacter in culture .only three samples were positive for Campylobacter genus and C.jejuni specific primer but none of them were positive for C.coli .99 samples were positive by universal primer of 16srDNA . Conclusion: The results indicate that C.jejuni isn't a prevalent agent in diarrhea in our region. Key words: Campylobacter jejuni -Gorgan- Diarrhea
Keywords: Key words: Campylobacter jejuni -Gorgan- Diarrhea
Full-Text [PDF 114 kb]   (2189 Downloads)    
Type of Study: Research | Subject: Special
Received: 2011/08/27 | Accepted: 2014/01/12 | Published: 2014/01/12
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Ziaei N, Amir Mozafari N, Kouhsari H, Moradi A, Tabarai A, Dadgar T, et al . Prevalence of Campylobacter jejuni in Diarrhea samples in Gorgan ,East north of Iran. mljgoums. 2008; 2 (2)
URL: http://goums.ac.ir/mljgoums/article-1-45-en.html
Volume 2, Number 2 (Autumn – Winter 2009[PERSIAN] 2008) Back to browse issues page
مجله علوم آزمایشگاهی دانشکده پیراپزشکی گرگان Medical Laboratory Journal
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