Volume 10, Issue 6 (Nov-Dec-2016 2016)                   mljgoums 2016, 10(6): 38-42 | Back to browse issues page

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Hashemzadeh Farhang H, Shahbazi P. Production of Specific Egg Yolk Antibody (IgY) against Cryptosporidium parvum Oocysts. mljgoums 2016; 10 (6) :38-42
URL: http://mlj.goums.ac.ir/article-1-920-en.html
Production of Specific Egg Yolk Antibody (IgY) against Cryptosporidium parvum Oocysts
Hossein Hashemzadeh Farhang1 , Parisa Shahbazi 2
1- Department of Pathobiology, Tabriz Branch
2- Department of Pathobiology, Faculty of Veterinary Medicine , p.shahbazi56@ gmail.com
Abstract:   (10816 Views)

ABSTRACT

         Background and Objectives: Cryptosporidium parvum is a coccidian protozoan that causes diarrhea in immunocompromised humans and newborn animals. The severity of the disease depends on the immunological status of the affected. Cryptosporiosis can have lethal effects on immunocompromised individuals such as AIDS patients. About 10% of AIDS patients die following an infection with C. parvum. Since there is no efficient treatment for cryptosporidiosis, there is an urgent need to search for more effective and safer alternatives. IgY is an avian immunoglobulin found in egg yolks. Due to its several advantages, IgY technology has been successfully used in biomedical research on humans and animals in the recent years. In this study, the specific chicken egg yolk antibody (IgY) against C. parvum whole oocyst antigens was produced and examined.

         Methods: The effect of specific chicken egg yolk antibody (IgY) against whole oocyst antigens was examined. IgY sample was obtained from eggs of chickens immunized with C. parvum whole oocyst antigens and analyzed with lysate of C. parvum oocysts by dot blot assay.

         Results: The IgY was produced with concentration of 9.7 mg/ml. This antibody was able to recognize the whole oocyst antigens until the dilution of 1:1000, but the best dilution for other immunoassays was 1:500.

         Conclusion: Since chicken egg yolk is a cheap and convenient source for mass production of specific antibodies, the use of IgY against whole oocyst antigens could be considered a suitable candidate for passive immunization against cryptosporidiosis in humans and animals.

         Keywords: Cryptosporidium parvum, IgY, Oocysts.

Keywords: Cryptosporidium parvum, IgY, Oocysts.
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Research Article: Original Paper |
Received: 2016/01/25 | Accepted: 2016/01/25 | Published: 2016/09/25 | ePublished: 2016/09/25
References
1. Xu YQ, Liu ZC. Therapeutic potential of adult bone marrow stem cells in liver disease and delivery approaches. Stem cell reviews. 2008; 4(2): 101-12. doi: 10.1007/s12015-008-9019-z. [DOI:10.1007/s12015-008-9019-z]
2. Baksh D, Song L, Tuan R. Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy. Journal of cellular and molecular medicine. 2004; 8(3): 301-16. PMID:15491506. [DOI:10.1111/j.1582-4934.2004.tb00320.x]
3. Pournasr B, Farzaneh Z, Shahsanvani M, Baharvand H. Liver development and in vitro differentiation of embryonic stem cells to hepatocytes. Yakhteh Medical Journal. 2010; 11(4): 348-73. [persian]
4. Banas A, Yamamoto Y, Teratani T, Ochiya T. Stem cell plasticity: learning from hepatogenic differentiation strategies. Developmental Dynamics. 2007; 236(12): 3228-41. [DOI:10.1002/dvdy.21330]
5. Teramoto K, Asahina K, Kumashiro Y, Kakinuma S, Chinzei. Hepatocyte differentiation from embryonic stem cells and umbilical cord blood cells. Journal of hepato-biliary-pancreatic surgery. 2005; 12(3): 196-202. PMID:15995807. [DOI:10.1007/s00534-005-0980-5]
6. Sugaya K. Potential use of stem cells in neuroreplacement therapies for neurodegenerative diseases. International review of cytology. 2003; 228: 1-30. PMID:14667041
7. Chapel A BJ, Bensidhoum M, Fouillard L, Young RG, Frick J, Demarquay C, et al. Mesenchymal stem cells home to injured tissues when co-infused with hematopoietic cells to treat a radiation-induced multi-organ failure syndrome. The journal of gene medicine. 2003; 5(12):1028-38. [DOI:10.1002/jgm.452]
8. Vater C, Kasten P, Stiehler M. Culture media for the differentiation of mesenchymal stromal cells. Acta biomaterialia. 2011; 7(2): 463-77. doi: 10.1016/j.actbio.2010.07.037. [DOI:10.1016/j.actbio.2010.07.037]
9. Mafi R, Hindocha S, Mafi P, Griffin M, Khan W. Suppl 2: Sources of Adult Mesenchymal Stem Cells Applicable for Musculoskeletal Applications-A Systematic Review of the Literature. The open orthopaedics journal. 2011; 5: 242. doi: 10.2174/1874325001105010242. [DOI:10.2174/1874325001105010242]
10. Bunnell BA, Flaat M, Gagliardi C, Patel B, Ripoll C. Adipose-derived stem cells: isolation, expansion and differentiation. Methods. 2008; 45(2): 115-20. doi: 10.1016/j.ymeth.2008.03.006. [DOI:10.1016/j.ymeth.2008.03.006]
11. Parolini O, Alviano F, Bergwerf I, Boraschi D, De Bari C, De Waele P, et al. Toward cell therapy using placenta-derived cells: disease mechanisms, cell biology, preclinical studies, and regulatory aspects at the round table. Stem cells and development. 2010;19(2): 143-54. doi: 10.1089/scd.2009.0404. [DOI:10.1089/scd.2009.0404]
12. Zhang Y, Li C, Jiang X, Zhang S, Wu Y, Liu B, et al. Human placenta-derived mesenchymal progenitor cells support culture expansion of long-term culture-initiating cells from cord blood CD34+ cells. Experimental hematology. 2004; 32(7): 657-64. [DOI:10.1016/j.exphem.2004.04.001]
13. Solomon A, Wajngarten M, Alviano F, Anteby I, Elchalal U, Pe'er J, et al. Suppression of inflammatory and fibrotic responses in allergic inflammation by the amniotic membrane stromal matrix. Clinical & Experimental Allergy. 2005; 35(7): 941-8. [DOI:10.1111/j.1365-2222.2005.02285.x]
14. Ilancheran S, Michalska A, Peh G, Wallace EM, Pera M, Manuelpillai U. Stem cells derived from human fetal membranes display multilineage differentiation potential. Biology of reproduction. 2007; 77(3): 577-88. [DOI:10.1095/biolreprod.106.055244]
15. NematiSh BH. Differentiation of human bone marrow mesenchymal stem cells to neural- like cells in vitro. Tehran University Medical JournalNov 2009;Vol. 67, No. 8 .527-534.
16. Eslaminejad MB, Nikmahzar A, Taghiyar L, Nadri S, Massumi M. Murine mesenchymal stem cells isolated by low density primary culture system. Development, growth & differentiation. 2006; 48(6): 361-70. [DOI:10.1111/j.1440-169X.2006.00874.x]
17. Solati Sarvandi S, Joghataei MT, Parivar K, Khosravi M, Sarveazad A, Sanadgol N. In vitro differentiation of rat mesenchymal stem cells to hepatocyte lineage. Iran J Basic Med Sci. 2015; 18: 89-97.
18. Raoufi A, Amini A, Azadbakht M, Abouzari M. In vitro differentiation of human umbilical vein mesenchymal stem cells into hepatocyte-like cells identified by cellular uptake of indocyanine green. Khoon. 2010; 7(1): 34-40. [Persian]
19. Ko O, Gerson S, Cooper B, Dyhouse S, Haynesworth S, Caplan A, et al. Rapid hematopoietic recovery after confusion of autologous-blood stem cells in advanced breast cancer patients receiving high dose chemotherapy. J Clin Oncol. 2000; 18(2): 307-16. [DOI:10.1200/JCO.2000.18.2.307]
20. Petite H, Viateau V, Bensaid W, Meunier A, de Pollak C, Bourguignon M, et al. Tissue-engineered bone regeneration. Nature biotechnology. 2000; 18(9): 959-63. [DOI:10.1038/79449]
21. Quarto R, Mastrogiacomo M, Cancedda R, Kutepov SM, Mukhachev V, Lavroukov A, et al. Repair of large bone defects with the use of autologous bone marrow stromal cells. New England Journal of Medicine2001;344(5):385-6. [DOI:10.1056/NEJM200102013440516]
22. Grinnemo K, Mansson A, Dellgren G, Klingberg D, Wardell E, Drvota V, et al. Xenoreactivity and engraftment of human mesenchymal stem cells transplanted into infarcted rat myocardium. The Journal of thoracic and cardiovascular surgery. 2004; 127(5): 1293-300. PMID:15115985. [DOI:10.1016/j.jtcvs.2003.07.037]
23. Horwitz EM GP, Koo WK, Marx JC, NeelMD,mcNallRY,MuulL,Hofmann T. Isolated allogenicbone marrow- drivedmesenchymal cells engraftand stimulate growth in children with osteogenesisimperfecta: Implications for cell therapy of bone. Proc Natl Acad Sci USA. 2002; 99: 8932-37. [DOI:10.1073/pnas.132252399]
24. Niknejad H, Peirovi H, Ahmadiani A, Ghanavi J, Jorjani M. Differentiation Factors That Influence Neuronal Markers Expression In Vitro From Human Amniotic Epithelial Cells. Eur Cell Mater. 2010; 19: 22-9. [DOI:10.22203/eCM.v019a03]
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