Abstract

Background and Objectives: Pseudomonas aeruginosa is the most common organism, which is separated from the burn infections.  Due to increased antibiotic resistance, there are many problems to deal with the infections caused by Pseudomonas aeruginosa. This study aimed to determine the resistance to antibiotics against clinical isolates of Pseudomonas using phenotype methods.

Material and Methods: 100 strains of Pseudomonas aeruginosa were collected from the burn patients in Taleghani hospital in Ahwaz, Iran, during a six-month period. After phenotypically initial identification, antibiotic sensitivity of isolated strains  to conventional antibiotics against Pseudomonas aeruginosa was determined using a disk diffusion technique,  and Phenotypic screening for MBLs production was  performed.

Results: the maximum percentage was related to   wound infection and the frequencies of the resistance to  imipenem, meropenem, piperacillin, piperacillin-tazobactam, ceftazidime, gentamicin, amikacin, and ciprofloxacin, doripenem, ertapenem and colistin sulphate, were 70%, 53%, 83%, 67%, 91%, 88%, 84%, 84%, 33%, 90%, and 0%,  respectively. The use of CD Test methods was approved for determining resistance to Carbapenems.

Conclusion: antibiotic resistance to Pseudomonas aeruginosa is increasing and colistin sulphate is the most effective antibiotic.

Keywords: Pseudomonas Aeruginosa; Burn Infection; Antibiotic Resistance.