Kashani, L, Okhly, M, Ghaemi, EA, Behnampour, N., Kashani, E, Okhly, HO, et al . Antibacterial Effect of Human Amnion Membrane. mljgoums. 2015; 8 (5) :28-35
URL:
http://goums.ac.ir/mljgoums/article-1-553-en.html
Kashani, L 1,
Okhly, M * 2,
Ghaemi, EA 3,
Behnampour, N. 4,
Kashani, E 5,
Okhly, HO 6,
Fendereski, S. 7,
Bazoori, M 8,
Falsafi, L 9
1- MD , General Practitioner, Infectious Disease Research Center, Golestan University of Medical Science, Gorgan, Iran
2- BSc , BSc of Microbiology, Infectious Disease Research Center, Golestan University of Medical Science, Gorgan, Iran , m_okhly@yahoo.com
3- PhD , Professor of Microbiology, Department of Microbiology, School of Medicine, Golestan University of Medical Science, Gorgan, Iran
4- PhD , Assistant Professor of Biostatistic, Department of Health, School of Health, Golestan University of Medical Sciences, Gorgan, Iran
5- MD , Associate Professor, Department of Gynecology, School of Medecine, Golestan University of Medical Science, Gorgan, Iran
6- BSc , BSc of Nursing, Nursing research Center, Golestan University of Medical Sciences, Gorgan, Iran
7- BSc , BSc of Microbiology, Infectious Disease Research Center, Golestan University of Medical Science, Gorgan, Iran
8- BSc , BSc of Microbiology , Infectious Disease Research Center, Golestan University of Medical Science, Gorgan, Iran
9- MSc , MSc of Computer, Golestan University of Medical Science, Gorgan, Iran
Abstract: (5470 Views)
Abstract Background and Objective: Along with antibiotics, the use of biological methods to combat bacteria is notably considered. A natural barrier such as amniotic membrane is one of the ways of dealing with bacterial infections. The aim of this study was to determine the antibacterial effect of human amniotic membrane. Materials and Methods: This descriptive study was performed in Dezyani teaching Hospital of Gorgan University of Medical Sciences, Iran. To evaluate the antibacterial activity against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli bacteria, 20 amniotic membranes were obtained from postpartum mothers and examined by repeated dilution, diffusion and extraction techniques. Data were collected by observation method and described by mean and standard deviation. Results: The antibacterial activity was found in 15% of the samples against Staphylococcus Aureus and Pseudomonas aeruginosa, while no antibacterial activity was found against E. coli. Given the 15% positive responses, "Diffusion" and "repeated dilution" techniques were more effective in investigating the antibacterial effect of amniotic membrane. Conclusion: The results show the probability of antimicrobial effect of amniotic membrane tissue and it seems that this property can be affected by many factors. Keywords: Amniotic Membrane, Anti-Bacterial Properties, Laboratory Methods
Type of Study:
Original Paper |
Subject:
Special Received: 2014/09/22 | Accepted: 2014/09/22 | Published: 2014/09/22