Detection of Methicillin-Resistant Staphylococcus Aureus by Phenotypical and Molecular Methods among Clinical Isolates

Zare, Z,; Solhjoo, K,; Norooznejad, MJ,; Kazemi, A,

Volume 8, Issue 4 (supplement Issue[PERSIAN] 2015) mljgoums 2015, 8(4): 7-13 | Back to browse issues page

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Zare, Z, Solhjoo, K, Norooznejad, MJ, Kazemi, A. Detection of Methicillin-Resistant Staphylococcus Aureus by Phenotypical and Molecular Methods among Clinical Isolates . mljgoums 2015; 8 (4) :7-13
URL: http://mlj.goums.ac.ir/article-1-530-en.html

Detection of Methicillin-Resistant Staphylococcus Aureus by Phenotypical and Molecular Methods among Clinical Isolates

Zare, Z¹

, Solhjoo, K

², Norooznejad, MJ¹

, Kazemi, A³

1- Islamic Azad University, Jahrom Branch, Jahrom, Iran
2- Department of Microbiology, Faculty of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran , solhiouk@yahoo.com
3- Faculty of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran

Abstract: (16152 Views)

Abstract Background and Objective: Increasing prevalence of methicillin resistant Staphylococcus aureus strains (MRSA) with their multidrug resistance potential causes difficulties in the treatment of infections due to these bacteria. Hence, the detection and determination of the frequency of MRSA strains via phenotypical and molecular methods is necessary in different parts of the county. Material and Methods: In this cross- sectional study, 150 Staphylococcus aureus strains were collected from different clinical samples in the hospitals located in Shiraz and Jahrom, Iran. To detect methicillin resistant Staphylococcus aureus strains, we used phenotypical methods such as disc diffusion and minimum inhibitory concentration by E-Test, and PCR molecular method for mass gene. Results: The frequency of methicillin resistant Staphylococcus aureus was 63 strains (42%) using disc diffusion and E-Test. while in PCR method, in addition to 63 strains, nine other isolates, which were sensitive to oxacillin by disc diffusion and E-Test, possessed also mecA gene. By and large, 72 isolates (48%) had methicillin resistance gene. Conclusion: Given the results of phenotypical and molecular methods, the frequency of methicillin resistant Staphylococcus aureus was relatively high in this area. Thus, the MRSA strains can be detectable as soon as possible by accurate and sensitive methods such as PCR to determinate the effective antibiotics. Keywords: Methicillin Resistant Staphylococcus Aureus, MRSA, MecA Gene, PCR

Keywords: Methicillin Resistant Staphylococcus Aureus, MRSA, MecA Gene, PCR

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Research Article: Original Paper |
Received: 2014/08/31 | Accepted: 2014/08/31 | Published: 2014/08/31 | ePublished: 2014/08/31