Volume 14, Issue 3 (May-Jun 2020)                   mljgoums 2020, 14(3): 26-32 | Back to browse issues page


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abedzadeh hajar A, dakhili M, saghazadeh M, aghaei S S, Nazari R. Synergistic Antifungal Effect of Fluconazole Combined with ZnO Nanoparticles against Candida albicans Strains from Vaginal Candidiasis. mljgoums 2020; 14 (3) :26-32
URL: http://mlj.goums.ac.ir/article-1-1221-en.html
1- Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran., Iran
2- Department of Lab Science, Faculty of Medicine, Qom Branch, Islamic Azad University, Qom, Iran., Iran , dr_dakhili@yahoo.com
Abstract:   (3911 Views)
Background and Objective: The prevalence of the infections caused by Candida species has led to a significant increase in their resistance to antifungal compounds. The aim of this study was to i) investigate drug resistance ii) evaluate the incidence of Candida albicans drug resistance pattern in the vaginal samples of women referring to health centers of Qom province, and iii) examine the effect of Zn nanoparticles combined with fluconazole against C. albicans isolates.
           Methods: This experimental, descriptive study was performed on 120 patients of candidiasis. In order to identify Candida albicans, direct experiments, differential culture, Germ tube test and sugar assimilation test (API20C kit) were conducted. The effect of different antifungal drugs and zinc nanoparticles and the synergistic effect of fluconazole with zinc nanoparticles were investigated by disk diffusion method. Minimum Inhibitory Concentration (MICs) of all cases was further specified.
           Results: Of the 120 samples, 50 (41.6%) were identified as Candida albicans. These strains were resistant to certain antifungal drugs while others were semi-sensitive and sensitive. The lowest and the highest mean diameter of inhibition zone in all Candida albicans isolates belonged to ketoconazole (15.64 mm) and fluconazole nano-ZnO (26.76mm), respectively. The lowest and the highest MICs were observed in fluconazole- nano-ZnO and nano-ZnO, respectively.
           Conclusion: The synergistic effect of Zn nanoparticles with fluconazole can be conducive to the treatment of vaginal candidiasis.
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Research Article: Original Paper | Subject: Microbiology
Received: 2019/05/27 | Accepted: 2019/07/31 | Published: 2020/04/30 | ePublished: 2020/04/30

References
1. Criseo G, Scordino F, Romeo O. Current methods for identifying clinically important cryptic Candida species. J Microbiol Methods. 2015; 111: 50-6. doi: 10.1016/j.mimet.2015.02.004. [DOI:10.1016/j.mimet.2015.02.004] [PubMed] [Google Scholar]
2. Rajabibazl M, Rasaee MJ, Nouri FM, Farahnejad Z. Development of agglutination test for detection of isolated mannoprotein antigen from Candida Albicans. 2013; 11(2): 89-95. [PubMed] [Google Scholar]
3. Ho K-m, Cheng T-s. Common superficial fungal infections-a short review. Med Bull. 2010; 15(11): 23-7. [Google Scholar]
4. Mohammadzadeh Rostami F, Shalibeik S, Rabi Nezhad Mousavi M. Molecular Characterization and Antibiotic Resistance Pattern of Nosocomial Clinical Isolates in Southeast of Iran. Medical Laboratory Journal. 2020 Jan 10;14(1):20-8. [DOI:10.29252/mlj.14.1.20] [Google Scholar]
5. Nasrollahi Z, Yadegari MH. Antifungal activity of caffeine in combination with fluconazole against Candida albicans. Infection, Epidemiology and Microbiology. 2016; 2(2): 18-21. [DOI:10.18869/modares.iem.2.2.18] [Google Scholar]
6. De Pádua RF, Guilhermetti E, Svidzinski TE. In vitro activity of antifungal agents on yeasts isolated from vaginal secretion. Acta Scientiarum. 2003; 25(1): 51-4. [Google Scholar]
7. Higa LH, Schilrreff P, Perez AP, Morilla MJ, Romero EL. The intervention of nanotechnology against epithelial fungal diseases. Journal of Biomaterials and Tissue Engineering. 2013; 3(1): 70-88. [DOI:10.1166/jbt.2013.1065] [Google Scholar]
8. Schmiedel Y, Zimmerli S. Common invasive fungal diseases: an overview of invasive candidiasis, aspergillosis, cryptococcosis, and Pneumocystis pneumonia. Swiss medical weekly. 2016;146:w14281. [DOI:10.4414/smw.2016.14281] [PubMed] [Google Scholar]
9. Kurtzman C, Fell JW, Boekhout T. The yeasts: a taxonomic study. 5ed: Elsevier. 2011; 1-292. [PubMed] [Google Scholar]
10. Hashemi S. Prevalence of candida and non-candida yeasts isolated from patients with yeast fungal infections in Tehran labs. Tehran Univ Med J. 2011; 69 (1) :55-62 [Google Scholar]
11. Evans EGV, Richardson MD. Medical mycology. A practical approach: IRL press; 1989. [Google Scholar]
12. Hooper RW, Ashcraft DS, Pankey GA. In vitro synergy with fluconazole plus doxycycline or tigecycline against clinical Candida glabrata isolates. Medical mycology. 2018; 57(1): 122-6. [DOI:10.1093/mmy/myy008] [PubMed] [Google Scholar]
13. Pathak AK, Jain NR, Joshi R. Antibiogram of Candida species isolated from mono and multi-species oral candidal carriage using disk diffusion method. Saudi Journal for Health Sciences. 2012; 1(3): 132-138. DOI: 10.4103/2278-0521.106082. [DOI:10.4103/2278-0521.106082] [Google Scholar]
14. Espinel-Ingroff A, Arthington-Skaggs B, Iqbal N, Ellis D, Pfaller M, Messer S, et al. Multicenter evaluation of a new disk agar diffusion method for susceptibility testing of filamentous fungi with voriconazole, posaconazole, itraconazole, amphotericin B, and caspofungin. J Clin Microbiol. 2007; 45(6): 1811-20. DOI: 10.1128/JCM.00134-07. [DOI:10.1128/JCM.00134-07] [PubMed] [Google Scholar]
15. Sharma RK, Ghose R. Synthesis of zinc oxide nanoparticles by homogeneous precipitation method and its application in antifungal activity against Candida albicans. Ceramics International. 2015; 41(1): 967-75. [DOI:10.1016/j.ceramint.2014.09.016] [Google Scholar]
16. Jehad Yousef M, Enas Danial N. In vitro antibacterial activity and minimum inhibitory concentration of zinc oxide and nano-particle zinc oxide against pathogenic strains. Journal of Health Sciences. 2012; 2(4): 38-42. [DOI:10.5923/j.health.20120204.04] [Google Scholar]
17. Stojković D, Soković M, Glamočlija J, Džamić A, Ćirić A, Ristić M, et al. Chemical composition and antimicrobial activity of Vitex agnus-castus L. fruits and leaves essential oils. Food Chemistry. 2011; 128(4): 1017-22. [DOI:10.1016/j.foodchem.2011.04.007] [Google Scholar]
18. Namazi A, Sahati F, Adibpour M, Mazloomi AS, Mohammad alizadeh S, Babapour J. Prevalence and Predisposing Factors to Candidiasis Infection in Women Supported by Health Centers of Tabriz, 2004. JSSU. 2007; 15(1): 54-60 [Google Scholar]
19. Feglo P, Narkwa P. Prevalence and antifungal susceptibility patterns of yeast isolates at the Komfo Anokye Teaching Hospital (KATH), Kumasi, Ghana. British Microbiology Research Journal. 2012; 2(1):10. [DOI:10.9734/BMRJ/2012/861] [Google Scholar]
20. Falahati M, Sharifinia S, Foroumadi A, Bolouri F, Akhlagh L, Yazdan Parast A, et al. Drug resistance pattern in Candida species isolated from vaginitis. Razi Journal of Medical Sciences. 2009; 16(65): 40-45. [Google Scholar]
21. Pakshir K, Akbarzadeh M, Bonyadpour B, Mohagheghzadeh A. In vitro activity and comparison of clotrimazol, fluconazol and nystatin against Candida vaginitis isolates in Shiraz, 2008. JRUMS. 2010; 9(3): 210-220. [Google Scholar]
22. Nazeri M, Mesdaghinia E, Moravej SAR, Atabakhshiyan R, Soleymani F. Prevalence of vulvovaginal candidiasis and frequency of candida species in women. Journal of Mazandaran University of Medical Sciences. 2012; 21(86): 254-62. [Google Scholar]
23. Abedzadeh AH, Dakhili M, Haghighi N, Khalilian M. An Investigation of the Prevalence of Candida Species in Women Referring to Health Center of Qom Province, Iran. Qom University Of Medical Sciences Journal. 2016; 9(12): 65-71. [Google Scholar]
24. Panchal P, Katara R, Mehta R, Soni S, Nanera A, Trivedi N, et al. Microbiological study of various candida species and its antifungal sensitivity testing isolated from antenatal women with vaginitis, in tertiary care teaching hospital, western india. International Journal of Microbiology Research. 2013; 5(6): 486. [DOI:10.9735/0975-5276.5.6.486-489] [Google Scholar]
25. Pfaller MA, Messer SA, Hollis RJ, Boyken L, Tendolkar S, Kroeger J, et al. Variation in susceptibility of bloodstream isolates of Candida glabrata to fluconazole according to patient age and geographic location in the United States in 2001 to 2007. J Clin Microbiol. 2009; 47(10): 3185-90. doi: 10.1128/JCM.00946-09. [DOI:10.1128/JCM.00946-09] [PubMed] [Google Scholar]
26. Shirzi NL, Shams Ghahfarokhi M, Yadgari MH. Evaluation of disk diffusion and broth microdilution methods for fluconazole susceptibility testing in one group of Candida spp. in Tehran. Daneshvar Medicine. 2008; 15(73): 51-58. [Google Scholar]
27. Kennedy H, Shankland G, Bagg J, Chalmers E, Gibson B, Williams C. Fluconazole and itraconazole susceptibilities of Candida spp. isolated from oropharyngeal specimens and blood cultures of paediatric haematology/oncology patients. Mycoses. 2006; 49(6): 457-62 DOI: 10.1111/j.1439-0507.2006.01272.x. [DOI:10.1111/j.1439-0507.2006.01272.x] [PubMed] [Google Scholar]
28. Pasquet J, Chevalier Y, Couval E, Bouvier D, Bolzinger M-A. Zinc oxide as a new antimicrobial preservative of topical products: Interactions with common formulation ingredients. Int J Pharm. 2015; 479(1): 88-95. doi: 10.1016/j.ijpharm.2014.12.031. [DOI:10.1016/j.ijpharm.2014.12.031] [PubMed] [Google Scholar]
29. Hosseini SS, Mohammadi SR, Joshaghani HR, Eskandari M. Antifungal effect of sodium dodecil sulfate and nano particle ZnO on growth inhibition of standard strain of Candida albicans. J Gorgan Univ Med Sci. 2011; 12(4): 64-69. [Google Scholar]

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