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mljgoums 2019, 13(5): 44-49 |
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ramezani S, peeri M, Azarbaijani M A, Dehghan F. Combined Effects of Regular Aerobic Exercise with Vitamin D Supplementation on Some Apoptosis Indices in the Lung of Rats Exposed to Hydrogen Peroxide. mljgoums 2019; 13 (5) :44-49
URL: http://mlj.goums.ac.ir/article-1-1190-en.html
URL: http://mlj.goums.ac.ir/article-1-1190-en.html
1- Dept. of Exercise Physiology, School of Physical Education and Sport Sciences, Central Tehran
2- Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran,IRAN , m.peeri@iauctb.ac.ir
3- physical education-azad university-Tehran-iran
4- Professor of Tehran University, Physiology
2- Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran,IRAN , m.peeri@iauctb.ac.ir
3- physical education-azad university-Tehran-iran
4- Professor of Tehran University, Physiology
Abstract: (4575 Views)
ABSTRACT
Background and Objectives: Exercise is a strong physiological stimulus that can affect apoptosis in the lungs by altering a number of extracellular and intracellular signaling pathways. The present study examined effects of regular aerobic exercise and vitamin D on expression of Bcl-2, Bax and caspase-3 in lung tissues of male rats exposed to hydrogen peroxide.
Methods: Forty-eight adult male Wistar rats were randomly assigned into six groups of eight, including 2 x H 2 O 2, 2H 2 O 2, Vit D (2HD), 2H2O2 + Regular Exercise Training (2HE), 2H2O2 + D3 + E (2HDE) ; Dimethyl sulfoxide (DMSO) and control (C). Subjects in the training groups performed aerobic exercise for eight weeks. Bax, Bcl-2, caspase-3 expression in the lung tissues was measured using RT-PCR.
Results: Bcl-2 expression in the exercise (P = 0.004) and vitamin D (P = 0.006) groups increased significantly compared to the control groups. Bax, Bcl-2 and caspase-3 expression was significantly lower in the exercise group and vitamin D supplementation group compared to the control group. On the other hand, concurrent exercise and vitamin D significantly reduce Bax expression but had no significant effect on Bcl-2 and caspase-3 expression.
Conclusion: Our results demonstrate that regular aerobic exercise along with vitamin D supplementation may play a role in reducing apoptosis in lungs following severe oxidative stress.
Keywords: Apoptosis, Bcl-2, Bax, Caspase3, Aerobic Exercise, Vitamin D
Research Article: Original Paper |
Subject:
Sport Physiology
Received: 2019/02/6 | Accepted: 2019/02/16 | Published: 2019/09/2 | ePublished: 2019/09/2
Received: 2019/02/6 | Accepted: 2019/02/16 | Published: 2019/09/2 | ePublished: 2019/09/2
References
1. Fedirko V, Bostick RM, Flanders WD, Long Q, Shaukat A, Rutherford RE, et al. Effects of Vitamin D and Calcium Supplementation on Markers of Apoptosis in Normal Colon Mucosa: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial. Cancer Prev Res (Phila). 2009; 2(3): 213-23. [DOI:10.1158/1940-6207.CAPR-08-0157]
2. Halliwell B, Gutteridge JMC. Free Radicals in Biology and Medicine. 3rd ed. Oxford University Press, Oxford. 1999; 1-35.
3. Magi B, Bargagli E, Bini L, Rottoli P. Proteome analysis of bronchoalveolar lavage in lung diseases. Proteomics. 2006; 6(23): 6354-69. [DOI:10.1002/pmic.200600303]
4. de Torre C, Ying SX, Munson PJ, Meduri GU, Suffredini AF. Proteomic analysis of inflammatory biomarkers in bronchoalveolar lavage. Proteomics. 2006; 6(13): 3949-57. [DOI:10.1002/pmic.200500693]
5. Aldini G, Yeum J, Niki E, Russell RM. Biomarkers for Antioxidant Defense and Oxidative Damage: Principles and Practical Applications. Ames, IA: Wiley-Blackwell. 2010. [DOI:10.1002/9780813814438]
6. Heidarvand L, Maali-Amiri R. Physio-biochemical and proteome analysis of chickpea in early phases of cold stress. J Plant Physiol. 2013; 170(5): 459-69. [DOI:10.1016/j.jplph.2012.11.021]
7. Franco R, Sanchez-Olea R, Reyes-Reyes EM, Panayiotidis MI. Environmental toxicity, oxidative stress and apoptosis: Menage a trois. Mutat Res. 2009; 674(1-2): 3-22. [DOI:10.1016/j.mrgentox.2008.11.012]
8. Weinmann M, Jendrossek V, Handrick R, Güner D, Goecke B, Belka C. Molecular ordering of hypoxia-induced apoptosis: critical involvement of the mitochondrial death pathway in a FADD/caspase-8 independent manner. Oncogene. 2004; 23(21): 3757-69. [DOI:10.1038/sj.onc.1207481]
9. Wei MC, Lindsten T, Mootha VK, Weiler S, Gross A, Ashiya M, et al. tBID, a membrane-targeted death ligand, oligomerizes BAK to release cytochrome c. Genes Dev. 2000; 14(16): 2060-2071.
10. Wei MC, Zong WX, Cheng EH, Lindsten T, Panoutsakopoulou V, Ross AJ, et al. Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death. Science. 2001; 292(5517): 727-730. [DOI:10.1126/science.1059108]
11. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell. 1997; 91(4): 479-489. [DOI:10.1016/S0092-8674(00)80434-1]
12. Srinivasula SM, Ahmad M, Fernandes-Alnemri T, Alnemri ES. Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization. Mol Cell. 1998; 1(7): 949-957. [DOI:10.1016/S1097-2765(00)80095-7]
13. Fuchs-Tarlovsky V. Role of antioxidants in cancer therapy. Nutrition. 2012; 29(1):15-21. [DOI:10.1016/j.nut.2012.02.014]
14. Barker T, Martins TB, Hill HR, Kjeldsberg CR, Dixon BM, Schneider ED, et al. Circulating proinflammatory cytokines are elevated and peak power output correlates with 25-hydroxyvitamin D in vitamin D insufficient adults. Eur J Appl Physiol. 2013; 113(6): 1523-34. [DOI:10.1007/s00421-012-2582-7]
15. Holick MF. Vitamin D deficiency. The New England Journal of Medicine. 2007; 357: 266-281. [DOI:10.1056/NEJMra070553]
16. Polidoro L1, Properzi G, Marampon F, Gravina GL, Festuccia C, Di Cesare E, et al. Vitamin D Protects Human Endothelial Cells from H2O2 Oxidant Injury Through the Mek/Erk-Sirt1 Axis Activation. J Cardiovasc Transl Res. 2013; 6(2): 221-31. [DOI:10.1007/s12265-012-9436-x]
17. Tashkin DP, Celli B, Senn S, Burkhart D, Kesten S, Menjoge S, et al. A 4-year trial of tiotropium in chronic obstructive pulmonary disease. N Engl J Med. 2008; 359(15): 1543-54. [DOI:10.1056/NEJMoa0805800]
18. Kuepper T, Morrison A, Gieseler U, Schoeffl V. Sport climbing with pre-existing cardiopulmonary medical conditions. Int J Sports Med. 2009; 30(6): 395-402. [DOI:10.1055/s-0028-1112143]
19. Vieira R, de Andrade V, Duarte A, Dos Santos A, Mauad T, Martins M, et al. Aerobic conditioning and allergic pulmonary inflammation in mice. II. Effects on lung vascular and parenchymal inflammation and remodeling. Am J Physiol Lung Cell Mol Physiol. 2008; 295(4): L670-9. [DOI:10.1152/ajplung.00465.2007]
20. Fisher-Wellman K, Bloomer RJ. Acute exercise and oxidative stress: a 30 year history. Dynamic Medicine. 2009; 8:1. [DOI:10.1186/1476-5918-8-1]
21. Quadrilatero J, Alway SE, Dupont-Versteegden EE. Skeletal muscle apoptotic response to physical activity: potential mechanisms for protection. Appl Physiol Nutr Metab. 2011; 36(5): 608-17. [DOI:10.1139/h11-064]
22. Li SF, Liu HX, Zhang YB, Yan YC, Li YP. The protective effects of alpha-ketoacids against oxidative stress on rat spermatozoa in vitro. Asian J Androl. 2010; 12(2): 247-56. doi: 10.1038/aja.2009.78. [DOI:10.1038/aja.2009.78]
23. Makino A, Skelton MM, Zou AP, Cowley AW Jr. Increased renal medullary H2O2 leads to hypertension. Hypertension. 2003; 42(1): 25-30. [DOI:10.1161/01.HYP.0000074903.96928.91]
24. Husain, Kazim, Stephen R Hazelrigg. Oxidative injury due to chronic nitric oxide synthase inhibition in rat: effect of regular exercise on the heart. Biochim Biophys Acta. 2002; 1587(1): 75-82. [DOI:10.1016/S0925-4439(02)00070-4]
25. Halder, Sunil K, Chakradhari Sharan, Ayman Al-Hendy. 1, 25-dihydroxyvitamin D3 treatment shrinks uterine leiomyoma tumors in the Eker rat model. Biol Reprod. 2012; 86(4): 116. [DOI:10.1095/biolreprod.111.098145]
26. Novelli EL, Diniz YS, Galhardi CM, Ebaid GM, Rodrigues HG, Mani F,et al. Anthropometrical parameters and markers of obesity in rats. Lab anim. 2007; 41(1): 111-119. [DOI:10.1258/002367707779399518]
27. Plant DR1, Gregorevic P, Warmington SA, Williams DA, Lynch GS. Endurance training adaptations modulate the redox-force relationship of rat isolated slow-twitch skeletal muscles. Clinical and experimental pharmacology and physiology. 2003; 30(1-2): 77-81. [DOI:10.1046/j.1440-1681.2002.03794.x]
28. Weiner P, Berar-Yanay N, Davidovich A, Magadle R, Weiner M. Specific inspiratory muscle training in patients with mild asthma with high consumption of inhaled beta(2)- agonists. Chest. 2000; 117(3): 722-7. [DOI:10.1378/chest.117.3.722]
29. Schieber M, Chandel NS. ROS function in redox signaling and oxidative stress. Curr Biol. 2014; 24(10): R453-62. [DOI:10.1016/j.cub.2014.03.034]
30. Wattenberg B, Lithgow T. Targeting of C-terminal (tail)-anchored proteins: understanding how cytoplasmic activities are anchored to intracellular membranes. Traffic. 2001; 2(1): 66-71. [DOI:10.1034/j.1600-0854.2001.20108.x]
31. Fernandes T, Magalhaes FC, Everton C. Aerobic exercise training inhibits skeletal muscular apoptotic signaling mediated by VEGF -VEGR2 in spontaneously hypertensive rats. Exercise and Sports Sciences 2012; 18)6): 412-418. [DOI:10.1590/S1517-86922012000600014]
32. Phaneuf S, leeuwenburgh Ch. Apoptosis and exercise. Medicine and Science in Sports and Exercise. 2001; 33(3): 393-39633. [DOI:10.1097/00005768-200103000-00010]
33. Bagci E, Vodovotz Y, Billiar T, Ermentrout G, Bahar I. Bistability in apoptosis: roles of bax ،bcl-2، and mitochondrial permeability transition pores. Biophysical Journal. 2006;90(5):1546-59. [DOI:10.1529/biophysj.105.068122]
34. Arslan S, Erdem S, Sivri A, Hasçelik Z, Tan E. Exercise-induced apoptosis of rat skeletal muscle and the effect of meloxicam. Rheumatology International. 2002; 21(4): 133-6. [DOI:10.1007/s00296-001-0156-9]
35. Packer N, Pervaiz N, Hoffman-Goetz L. Does exercise protect from cognitive decline by altering brain cytokine and apoptotic protein levels? A systematic review of the literature. Exerc Immunol Rev. 2010; 16: 138-62.
36. Fisher G, Schwartz DD, Quindry J, Barberio MD, Foster EB, Jones KW, et al. Lymphocyte enzymatic antioxidant responses to oxidative stress following high-intensity interval exercise. J Appl Physiol (1985). 2011; 110(3): 730-7. [DOI:10.1152/japplphysiol.00575.2010]
37. Podhorska-Okolow M، Dziegiel P ،Gomulkiewicz A، Kisiela D، Dolinska-Krajewska B، Jethon Z، et al. Exercise-induced apoptosis in rat kidney is mediated by both angiotensin II AT1 and AT2 receptors. Histol Histopathol. 2006; 21(5): 459-66.
38. Quadrilatero J, Bombardier E, Norris SM, Talanian JL, Palmer MS, Logan H, et al. Prolonged moderate-intensity aerobic exercise does not alter apoptotic signaling and DNA fragmentation in human skeletal muscle. American Journal of Physiology-Endocrinology and Metabolism. 2010; 298(3): E534-E547. [DOI:10.1152/ajpendo.00678.2009]
39. McMillan EM, Graham DA, Rush JW, Quadrilatero J. Decreased DNA fragmentation and apoptotic signaling in soleus muscle of hypertensive rats following 6 weeks of treadmill training. Journal of Applied Physiology. 2012; 113(7): 1048-1057. [DOI:10.1152/japplphysiol.00290.2012]
40. Buzina R, Suboticanec K. Vitamin C status and physical working capacity in adolescents. Int J Vitam Nutr Res. 1985; 7: 157-66.
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.