Volume 12, Issue 6 (Nov - Dec 2018)                   mljgoums 2018, 12(6): 28-33 | Back to browse issues page

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Bagheri R, Rashidlamir A, Attarzadeh Hosseini S R. Effect of Resistance Training with Blood Flow Restriction on Follistatin to Myostatin Ratio, Body Composition and Anaerobic Power of Trained-Volleyball Players. mljgoums. 2018; 12 (6) :28-33
URL: http://mlj.goums.ac.ir/article-1-1133-en.html
1- Department of Sports Physiology, Faculty of Sports Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
Abstract:   (3069 Views)
           Background and objectives: The present study was designed to determine the effect of blood flow restriction training (BFRT) on follistatin to myostatin ratio, body composition and anaerobic power of trained volleyball players.
           Methods: Eighteen trained volleyball players were randomly assigned into two study groups: resistance training with blood flow restriction (BFRT; n=9) and resistance training without blood flow restriction (WBFRT; n=9). The subjects performed trainings three sessions a week, for eight weeks. In each session, barbell squat, leg extension, leg curl and dumbbell lunges were performed in three sets of 15 repetitions, with rest intervals of 30 seconds. Serum follistatin and myostatin concentrations, body composition and anaerobic power were assessed before and after the study. Data were analyzed using the paired sample t-test, Wilcoxon test, independent sample t-test and Mann-Whitney U test. All statistical analyses were done in SPSS (version 22), and a P-value of less than 0.05 was considered statistically significant.
           Results: Follistatin levels increased significantly (P=0.001), while myostatin levels decreased significantly in both groups (P=0.001). Follistatin to myostatin ratio increased significantly in both groups (P=0.001). Although body fat percentage decreased in both groups, it did not differ significantly between the two groups (P=0.28). Moreover, anaerobic power increased significantly in both groups (P=0.001), but this increase was more profound in the BFRT group (P=0.001).
           Conclusion: Based on our findings, blood flow restriction can be applied as remarkable approach to boost body adaptation responses to resistance training.
           KEYWORDS: Resistance training, Myostatin, Follistatin, Blood flow restriction.
Full-Text [PDF 546 kb]   (466 Downloads)    
Type of Study: Original Paper | Subject: Biochemistry
Received: 2018/10/31 | Accepted: 2018/10/31 | Published: 2018/10/31 | ePublished: 2018/10/31

1. Gabbett T, Georgieff B, Domrow N. The use of physiological, anthropometric, and skill data to predict selection in a talent-identified junior volleyball squad. Journal of sports sciences. 2007;25(12):1337-44. [DOI:10.1080/02640410601188777]
2. Loenneke JP, Pujol TJ. The use of occlusion training to produce muscle hypertrophy. Strength & Conditioning Journal. 2009;31(3):77-84. [DOI:10.1519/SSC.0b013e3181a5a352]
3. Shimizu R, Hotta K, Yamamoto S, Matsumoto T, Kamiya K, Kato M, et al. Low-intensity resistance training with blood flow restriction improves vascular endothelial function and peripheral blood circulation in healthy elderly people. European journal of applied physiology. 2016;116(4):749-57. [DOI:10.1007/s00421-016-3328-8]
4. Thomas M, Langley B, Berry C, Sharma M, Kirk S, Bass J, et al. Myostatin, a negative regulator of muscle growth, functions by inhibiting myoblast proliferation. Journal of Biological Chemistry. 2000;275(51):40235-43. [DOI:10.1074/jbc.M004356200]
5. Kawada S, Tachi C, Ishii N. Content and localization of myostatin in mouse skeletal muscles during aging, mechanical unloading and reloading. Journal of Muscle Research & Cell Motility. 2001;22(8):627-33. [DOI:10.1023/A:1016366409691]
6. Lee S-J, McPherron AC. Regulation of myostatin activity and muscle growth. Proceedings of the National Academy of Sciences. 2001;98(16):9306-11. [DOI:10.1073/pnas.151270098]
7. SHIBANUMA M, MASHIMO Ji, MITA A, KUROKI T, NOSE K. Cloning from a mouse osteoblastic cell line of a set of transforming‐growth‐factor‐β1‐regulated genes, one of which seems to encode a follistatin‐related polypeptide. The FEBS Journal. 1993;217(1):13-9.
8. Tortoriello DV, Sidis Y, Holtzman DA, Holmes WE, Schneyer AL. Human follistatin-related protein: a structural homologue of follistatin with nuclear localization. Endocrinology. 2001;142(8):3426-34. [DOI:10.1210/endo.142.8.8319]
9. Santos A, Lamas L, Ugrinowitsch C, Tricoli V, Miyabara E, Soares A, et al. Different resistance-training regimens evoked a similar increase in myostatin inhibitors expression. International journal of sports medicine. 2015;36(09):761-8. [DOI:10.1055/s-0035-1547219]
10. Hosseini SRA, Moienneia N, Rad MM. The effect of two intensities resistance training on muscle growth regulatory myokines in sedentary young women. Obesity Medicine. 2017;5:25-8. [DOI:10.1016/j.obmed.2017.01.004]
11. Brzycki M. Strength testing—predicting a one-rep max from reps-to-fatigue. Journal of Physical Education, Recreation & Dance. 1993;64(1):88-90. [DOI:10.1080/07303084.1993.10606684]
12. Abe T, Yasuda T, Midorikawa T, Sato Y, CF K, Inoue K, et al. Skeletal muscle size and circulating IGF-1 are increased after two weeks of twice daily "KAATSU" resistance training. International Journal of KAATSU Training Research. 2005;1(1):6-12. [DOI:10.3806/ijktr.1.6]
13. Roth SM, Martel GF, Ferrell RE, Metter EJ, Hurley BF, Rogers MA. Myostatin gene expression is reduced in humans with heavy-resistance strength training: a brief communication. Experimental biology and medicine. 2003;228(6):706-9. [DOI:10.1177/153537020322800609]
14. Santos A, Neves Jr M, Gualano B, Laurentino G, Lancha Jr A, Ugrinowitsch C, et al. Blood flow restricted resistance training attenuates myostatin gene expression in a patient with inclusion body myositis. Biology of sport. 2014;31(2):121. [DOI:10.5604/20831862.1097479]
15. Hulmi JJ, Ahtiainen JP, Kaasalainen T, PöLLANEN E, Hakkinen K, Alen M, et al. Postexercise myostatin and activin IIb mRNA levels: effects of strength training. Medicine & Science in Sports & Exercise. 2007;39(2):289-97. [DOI:10.1249/01.mss.0000241650.15006.6e]
16. Allen DL, Unterman TG. Regulation of myostatin expression and myoblast differentiation by FoxO and SMAD transcription factors. American Journal of Physiology-Cell Physiology. 2007;292(1):C188-C99. [DOI:10.1152/ajpcell.00542.2005]
17. Loenneke JP, Abe T, Wilson JM, Ugrinowitsch C, Bemben MG. Blood flow restriction: how does it work? Frontiers in physiology. 2012;3:392. [DOI:10.3389/fphys.2012.00392]
18. Schiaffino S, Dyar KA, Ciciliot S, Blaauw B, Sandri M. Mechanisms regulating skeletal muscle growth and atrophy. The FEBS journal. 2013;280(17):4294-314. [DOI:10.1111/febs.12253]
19. Sandri M, Sandri C, Gilbert A, Skurk C, Calabria E, Picard A, et al. Foxo transcription factors induce the atrophy-related ubiquitin ligase atrogin-1 and cause skeletal muscle atrophy. Cell. 2004;117(3):399-412. [DOI:10.1016/S0092-8674(04)00400-3]
20. Kimball SR, Farrell PA, Jefferson LS. Invited Review: Role of insulin in translational control of protein synthesis in skeletal muscle by amino acids or exercise. Journal of Applied Physiology. 2002;93(3):1168-80. [DOI:10.1152/japplphysiol.00221.2002]
21. Jensky NE, Sims JK, Dieli-Conwright CM, Sattler FR, Rice JC, Schroeder ET. Exercise does not influence myostatin and follistatin mRNA expression in young women. Journal of strength and conditioning research/National Strength & Conditioning Association. 2010;24(2):522. [DOI:10.1519/JSC.0b013e3181c8664f]
22. Monikh K, Kashef M, Azad A, Ghasemnian A. Effects of 6 weeks resistance training on Body Composition, serum Leptin and muscle strength in non-athletic men. The Horizon of Medical Sciences. 2015;21(2):135-40. [DOI:10.18869/acadpub.hms.21.2.135]
23. Kwon HR, Han KA, Ku YH, Ahn HJ, Koo B-K, Kim HC, et al. The effects of resistance training on muscle and body fat mass and muscle strength in type 2 diabetic women. Korean diabetes journal. 2010;34(2):101-10. [DOI:10.4093/kdj.2010.34.2.101]
24. Nicklas BJ, Wang X, You T, Lyles MF, Demons J, Easter L, et al. Effect of exercise intensity on abdominal fat loss during calorie restriction in overweight and obese postmenopausal women: a randomized, controlled trial–. The American journal of clinical nutrition. 2009;89(4):1043-52. [DOI:10.3945/ajcn.2008.26938]
25. Kim JE, Chen J. Regulation of peroxisome proliferator–activated receptor-γ activity by mammalian target of rapamycin and amino acids in adipogenesis. Diabetes. 2004;53(11):2748-56. [DOI:10.2337/diabetes.53.11.2748]
26. Porstmann T, Santos CR, Griffiths B, Cully M, Wu M, Leevers S, et al. SREBP activity is regulated by mTORC1 and contributes to Akt-dependent cell growth. Cell metabolism. 2008;8(3):224-36. [DOI:10.1016/j.cmet.2008.07.007]
27. Park S, Kim JK, Choi HM, Kim HG, Beekley MD, Nho H. Increase in maximal oxygen uptake following 2-week walk training with blood flow occlusion in athletes. European journal of applied physiology. 2010;109(4):591-600. [DOI:10.1007/s00421-010-1377-y]
28. A abdolmaleki NB, A hemmatfat. Effect of strength training with and without vascular occlusion on anaerobic power of athletes and non-athletes. Quarterly Journal of Sport Sciences. 2014.
29. Bodine SC, Stitt TN, Gonzalez M, Kline WO, Stover GL, Bauerlein R, et al. Akt/mTOR pathway is a crucial regulator of skeletal muscle hypertrophy and can prevent muscle atrophy in vivo. Nature cell biology. 2001;3(11):1014. [DOI:10.1038/ncb1101-1014]
30. Loenneke J, Fahs C, Wilson J, Bemben M. Blood flow restriction: the metabolite/volume threshold theory. Medical hypotheses. 2011;77(5):748-52. [DOI:10.1016/j.mehy.2011.07.029]
31. Takarada Y, Nakamura Y, Aruga S, Onda T, Miyazaki S, Ishii N. Rapid increase in plasma growth hormone after low-intensity resistance exercise with vascular occlusion. Journal of applied physiology. 2000;88(1):61-5. [DOI:10.1152/jappl.2000.88.1.61]
32. Kiang JG, Tsokos GC. Heat shock protein 70 kDa: molecular biology, biochemistry, and physiology. Pharmacology & therapeutics. 1998;80(2):183-201. [DOI:10.1016/S0163-7258(98)00028-X]
33. Lang F, Busch GL, Ritter M, Volkl H, Waldegger S, Gulbins E, et al. Functional significance of cell volume regulatory mechanisms. Physiological reviews. 1998;78(1):247-306. [DOI:10.1152/physrev.1998.78.1.247]
34. Silvagno F, Xia H, Bredt DS. Neuronal nitric-oxide synthase-, an alternatively spliced isoform expressed in differentiated skeletal muscle. Journal of Biological Chemistry. 1996;271(19):11204-8. [DOI:10.1074/jbc.271.19.11204]

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