Volume 12, Issue 5 (Sep-Oct 2018)                   mljgoums 2018, 12(5): 10-15 | Back to browse issues page


XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Yazdi M, Bouzari M, Ghaemi E A. Detection of fim, pap, sfa and afa Adhesin-Encoding Operons in Escherichia coli Strains Isolated from Urinary Tract Infections. mljgoums. 2018; 12 (5) :10-15
URL: http://mlj.goums.ac.ir/article-1-1114-en.html
1- Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran
2- Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran , bouzari@sci.ui.ac.ir
3- Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Abstract:   (4467 Views)
ABSTRACT
             Background and objectives: Urinary tract infections (UTIs) are one of the most common infectious diseases caused by bacteria. The primary etiologic agent of UTIs is Escherichia coli. Uropathogenic E.coli (UPEC) strains have a number of specific virulence factors, which can worsen UTIs. This study was performed to detect fim, pap, sfa and afa genes among E.coli strains isolated from UTIs.
             Methods: A total of 100 E. coli isolates from patients with UTI was collected between June and December 2015 from Mosavi and Sayyad Shirazi hospitals in Gorgan, Iran. All bacterial isolates were identified via standard biochemical testing and Gram straining. Presence of the genes was assessed by polymerase chain reaction.
             Results: The frequency of the fim, pap, sfa and afa genes was 100%, 79%, 69% and 8%, respectively. All isolates contained at least one virulence gene. Prevalence of multiple adhesion genes was 6% for all genes and 65% for three genes (fim, pap and sfa) together. In addition, the frequency of the fim gene was significantly higher than that of the other genes (P<0.0001).
             Conclusion: The results of this study indicate the high prevalence of virulence factors that can enhance pathogenicity of E. coli. Therefore, these factors could be used as diagnostic markers or vaccine targets.
             Keywords: Virulence factors, Urinary tract infection, Uropathogenic Escherichia coli.
Full-Text [PDF 561 kb]   (457 Downloads)    
Type of Study: Review Article |
Received: 2018/08/13 | Accepted: 2018/08/13 | Published: 2018/08/13 | ePublished: 2018/08/13

References
1. Hojati Z, Zamanzad B, Hashemzadeh M, Molaie R, Gholipour A. The FimH gene in uropathogenic Escherichia coli strains isolated from patients with urinary tract infection. Jundishapur J Microbiol. 2015; 8 (2): e17520. doi: 10.5812/jjm.17520. [DOI:10.5812/jjm.17520]
2. Tiba MR, Yano T, Leite DdS. Genotypic characterization of virulence factors in Escherichia coli strains from patients with cystitis. Rev Inst Med Trop Sao Paulo. 2008; 50(5): 255-60. [DOI:10.1590/S0036-46652008000500001]
3. Karimian A, Momtaz H, Madani M. Detection of uropathogenic Escherichia coli virulence factors in patients with urinary tract infections in Iran. Afr J Microbiol Res. 2012; 6(39): 6811-6. DOI: 10.5897/AJMR12.1462. [DOI:10.5897/AJMR12.1462]
4. Kulkarni R, Dhakal BK, Slechta ES, Kurtz Z, Mulvey MA, Thanassi DG. Roles of putative type II secretion and type IV pilus systems in the virulence of uropathogenic Escherichia coli. PLoS One. 2009;4(3):e4752. doi: 10.1371/journal.pone.0004752. [DOI:10.1371/journal.pone.0004752]
5. Saki A, Mirzaee M. Frequency of Fimbrial Virulence Genes (fim, pap, sfa) in Escherichia Coli Isolated from the Patients with Urinary Tract Infections. J Shahid Sadoughi Univ Med Sci. 2017; 24(11): 913-923.
6. Bien J, Sokolova O, Bozko P. Role of uropathogenic Escherichia coli virulence factors in development of urinary tract infection and kidney damage. Int J Nephrol. 2012; 2012:681473. [DOI:10.1155/2012/681473]
7. Rahdar M, Rashki A, Miri HR, Ghalehnoo MR. Detection of pap, sfa, afa, foc, and fim adhesin-encoding operons in uropathogenic Escherichia coli isolates collected from patients with urinary tract infection. Jundishapur J Microbiol. 2015; 8(8): e22647. doi: 10.5812/jjm.22647. [DOI:10.5812/jjm.22647]
8. Donnenberg MS, Welch RA. Virulence determinants of uropathogenic Escherichia coli: Urinary tract infections: molecular pathogenesis and clinical management. ASM Press, Washington, DC; 1996.
9. Johnson JR. Virulence factors in Escherichia coli urinary tract infection. Clin Microbiol Rev. 1991; 4(1): 80-128. [DOI:10.1128/CMR.4.1.80]
10. Tajbakhsh E, Ahmadi P, Abedpour-Dehkordi E, Arbab-Soleimani N, Khamesipour F. Biofilm formation, antimicrobial susceptibility, serogroups and virulence genes of uropathogenic E. coli isolated from clinical samples in Iran. Antimicrob Resist Infect Control. 2016; 5(1):11. [DOI:10.1186/s13756-016-0109-4]
11. Snyder JA, Lloyd AL, Lockatell CV, Johnson DE, Mobley HL. Role of phase variation of type 1 fimbriae in a uropathogenic Escherichia coli cystitis isolate during urinary tract infection. Infect Immun. 2006; 74(2): 1387-93. [DOI:10.1128/IAI.74.2.1387-1393.2006]
12. Knöbl T, Gomes T, Vieira M, Bottino J, Ferreira A. Detection of pap, sfa and fim adhesin-encoding operons in avian pathogenic Escherichia coli. Int J Appl Res Vet Med. 2004;2:135-141.
13. Ott M, Hoschützky H, Jann K, Van Die I, Hacker J. Gene clusters for S fimbrial adhesin (sfa) and F1C fimbriae (foc) of Escherichia coli: comparative aspects of structure and function. J Bacteriol. 1988;170(9):3983-90. [DOI:10.1128/jb.170.9.3983-3990.1988]
14. Le Bouguenec C, Archambaud M, Labigne A. Rapid and specific detection of the pap, afa, and sfa adhesin-encoding operons in uropathogenic Escherichia coli strains by polymerase chain reaction. J Clin Microbiol. 1992;30(5):1189-93.
15. Shetty A, Kumar S, Shekar M, Shetty A, Karunasagar I. Prevalence of adhesive genes among uropathogenic Escherichia coli strains isolated from patients with urinary tract infection in Mangalore. Indian J Med Microbiol. 2014;32(2):175. [DOI:10.4103/0255-0857.129812]
16. Dash SK, Chakraborty SP, Mandal D, Roy S. Isolation and characterization of multi drug resistant uropathogenic Escherichia coli from urine sample of urinary tract infected patients. Int J Life Sci Pharma Res. 2012;2:25-39.
17. Bagheri H, Ghaemi A, Aslani M, Mozafari N, Livani S, Dadgar T. The Prevalence of Enteroaggregative Escherichia coli in cases of Diarrhea in Gorgan, IRAN. Med Lab J. 2008;2(1):70.
18. Pusz P, Bok E, Mazurek J, Stosik M, Baldy-Chudzik K. Type 1 fimbriae in commensal Escherichia coli derived from healthy humans. Acta Biochim Pol. 2014;61(2):389-92.
19. Blanco M, Blanco J, Alonso M, Mora A, Balsalobre C, Munoa F, et al. Detection of pap, sfa and afa adhesin-encoding operons in uropathogenic Escherichia coli strains: relationship with expression of adhesins and production of toxins. Res Microbiol. 1997;148(9):745-55. [DOI:10.1016/S0923-2508(97)82450-3]
20. Nazemi A, Naderi M, Jafarpour M, Mirinargesi M, Sharifi SH. The Detection of Fimbrial Pathogenic Genes in E.coli Strains Isolated from Patients with Urinary Tract Infection. Med Lab J. 2010; 4(2):31–7.
21. Antão E-M, Wieler LH, Ewers C. Adhesive threads of extraintestinal pathogenic Escherichia coli. Gut Pathog. 2009;1(1):22. [DOI:10.1186/1757-4749-1-22]
22. Farshad S, Emamghorashi F. The prevalence of virulence genes of E. coli strains isolated from children with urinary tract infection. Saudi J Kidney Dis Transpl. 2009;20(4):613.
23. Garofalo CK, Hooton TM, Martin SM, Stamm WE, Palermo JJ, Gordon JI, et al. Escherichia coli from urine of female patients with urinary tract infections is competent for intracellular bacterial community formation. Infect Immun. 2007;75(1):52-60. [DOI:10.1128/IAI.01123-06]
24. Watts RE, Hancock V, Ong C-LY, Vejborg RM, Mabbett AN, Totsika M, et al. Escherichia coli isolates causing asymptomatic bacteriuria in catheterized and noncatheterized individuals possess similar virulence properties. J Clin Microbiol. 2010;48(7):2449-58. [DOI:10.1128/JCM.01611-09]
25. Silveira WDd, Benetti F, Lancellotti M, Ferreira A, Solferini VN, Brocchi M. Biological and genetic characteristics of uropathogenic Escherichia coli strains. Rev Inst Med Trop Sao Paulo. 2001;43(6):303-10. [DOI:10.1590/S0036-46652001000600001]
26. Dobrindt U, Blum-Oehler G, Hartsch T, Gottschalk G, Ron EZ, Fünfstück R, et al. S-Fimbria-Encoding Determinant sfa I Is Located on Pathogenicity Island III536 of UropathogenicEscherichia coli Strain 536. Infect Immun. 2001;69(7):4248-56. [DOI:10.1128/IAI.69.7.4248-4256.2001]

Add your comments about this article : Your username or Email:
CAPTCHA code

Send email to the article author


© 2007 All Rights Reserved | Medical Laboratory Journal