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1- Department of Biochemistry, Faculty of Basic Science, Razi University Kermanshah, Iran, Department of Biochemistry, Faculty of Basic Science, Razi University Kermanshah, Iran
2- Veterinary Medicine, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran, Veterinary Medicine, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran , Dr_Abbasi_m@yahoo.com
Abstract:   (944 Views)
Background and Objective: The global prevalence of prostate cancer is remarkably high and it is considered a problematic malignancy in men due to urinary problems and high mortality rates. Various genes in prostate cancer have been examined regarding their diagnostic value. However, KLK2 and KLK3 genes, which have high nucleotide homology, have not been studied in Iran. Hence, the aim of this study was to evaluate the expression of these genes in Iranian patients.
Methods: In this case-control study, three groups consisting of healthy individuals, patients with benign prostatic hyperplasia and patients with prostate cancer were studied. Peripheral blood samples of 2.5 ml were collected from individuals and immediately transferred to the laboratory under appropriate temperature conditions. After cell lysis, mRNA molecules were extracted using an extraction kit and cDNA was synthesized subsequently. Finally, Real-Time PCR was performed and gene expression level was measured in comparison with 18sRNA for each individual.
Results: Based on the results, the expression of both genes was altered; analysis of CT revealed that KLK2 gene expression was specifically increased in patients with cancer, and was significantly different from that of the control group, but not from gene expression in patients with benign prostatic hyperplasia. On the other hand, the relative expression of KLK3 gene in individuals with benign prostatic hyperplasia was higher than that in control and cancer groups. The difference in the expression of KLK3 in control and cancer groups was not significant. The results of ΔCt for KLK2 were similar to the results of CT for this gene while for KLK3, the difference among all three groups was significant.
Conclusions: The results of this study showed that the analysis of KLK2 gene expression has priority over the analysis of KLK3 in diagnosis and differentiation of normal individuals, patients with benign prostatic hyperplasia and patients with prostate cancer. It was also found that KLK3 gene had the highest level of expression in the group of benign prostate hyperplasia and as a result, this gene is not cancer-specific.
Keywords: Prostate Cancer, Benign Prostatic Hyperplasia, Kallikrein, Gene Expression
     
Type of Study: Original Paper | Subject: Special
Received: 2018/07/8 | Accepted: 2018/07/8 | Published: 2018/07/8

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