Volume 12, Issue 4 (Jul-Aug 2018)                   mljgoums 2018, 12(4): 7-11 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ebrahimi A, Daryalal Y, Mahzounieh M, Lotfalian S. Effects of Sub-Minimum Inhibitory Concentrations of Silver Nanoparticles on Some Virulence Factors of Staphylococcus aureus . mljgoums. 2018; 12 (4) :7-11
URL: http://mlj.goums.ac.ir/article-1-1091-en.html
1- Department of Pathobiology, School of Veterinary Science, Shahrekord University, Shahrekord, Iran , A_kahrizsangi@yahoo.com
2- Department of Pathobiology, Veterinary College, Shahrekord University, Shahrekord, Iran
3- Department of Pathobiology, School of Veterinary Science, Shahrekord University, Shahrekord, Iran
4- Department of Pathobiology, School of Veterinary Science, Shahrekord University, Shahrekord, , Iran
Abstract:   (7286 Views)
         Background and Objectives: Silver nanoparticles (AgNPs) have physical and surface properties that could threaten human and environmental health. AgNPs are classified as ‘very toxic’ to eukaryotic organisms and are less toxic to bacteria. The aim of the present study was to study the effects of different sub-minimum inhibitory concentrations (MICs) of AgNPs on some virulence factors of Staphylococcus aureus as a pathogenic bacterial model.
         Methods: Tube double serial dilution method was used to determine MIC of AgNPs against standard strain and ten field isolates of S. aureus. Tube cultures of isolates in LB broth were supplemented with different concentrations of AgNPs and were incubated at 37 °C with constant shaking under aerobic conditions. Samples from each tube were streaked on blood agar plates and assay for hemolysins, coagulase and DNase production were performed.
         Results: The MIC of AgNPs against all examined isolates was determined as 50 µg/mL. The results showed that 1/2, 1/4 and 1/8 MIC of AgNPs had no negative effect on DNase and coagulase production but inhibited alpha- and beta-hemolysin production in most isolates (64-91%). In addition, production of delta-hemolysin was inhibited by 1/2 MIC of AgNPs.
         Conclusion: The effects of sub-MIC of AgNPs on bacterial growth appear at 4-8 hours post-exposure and then the bacteria follow a normal growth trend. This toxic effect may affect ecosystems species.
         Keywords: Silver particles, Minimum inhibitory concentration, Virulence factors, Staphylococcus aureus.
Full-Text [PDF 616 kb]   (889 Downloads)    
Type of Study: Original Paper |
Received: 2018/06/3 | Accepted: 2018/06/3 | Published: 2018/06/3 | ePublished: 2018/06/3

1. Park S, Lee YK, Jung M, Kim KH, Chung N, Ahn EK, et al. Cellular toxicity of various inhalable metal nanoparticles on human alveolar epithelial cells. Inhalation Toxicology. 2007;19(sup1): 59-65. DOI:10.1080/08958370701493282. [DOI:10.1080/08958370701493282]
2. Boenigk J, Beisser D, Zimmermann S, Bock C, Jakobi J, Grabner D, et al. Effects of silver nitrate and silver nanoparticles on a planktonic community: general trends after short-term exposure. PloS one. 2014; 9(4):e95340. DOI: 10.1371/journal.pone.0095340. [DOI:10.1371/journal.pone.0095340]
3. Bondarenko O, Juganson K, Ivask A, Kasemets K, Mortimer M, Kahru A. Toxicity of Ag, CuO and ZnO nanoparticles to selected environmentally relevant test organisms and mammalian cells in vitro: a critical review. Archives of Toxicology. 2013; 87(7): 1181-200. DOI: 10.1007/s00204-013-1079-4. [DOI:10.1007/s00204-013-1079-4]
4. Quinn PJ, Markey BK, Leonard FC, FitzPatrick ES, Fanning S, Hartigan P. Veterinary microbiology and microbial disease. John Wiley & Sons. 2011; 539- 560.
5. Ebrahimi A, Ghasemi M, Ghasemi B. Some virulence factors of Staphylococci isolated from wound and skin infections in Shahrekord, IR Iran. Jundishapur Journal of Microbiology. 2014;7(4). e9225. DOI: 10.5812/jjm.9225. [DOI:10.5812/jjm.9225]
6. Murray PR, Baron EJ, Jorgensen J, Landry M, Pfaller M. Manual of clinical microbiology. 9th ed. 2006; 1-1267.
7. Hebert G, Hancock GA. Synergistic hemolysis exhibited by species of staphylococci. Journal of clinical microbiology. 1985; 22(3): 409-15.
8. Wayne P. Clinical and Laboratory Standarts Institute: Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that grow aerobically. Approved Standart M7-A7, CLSI, USA; 2006. DOI: 10.1007/s13273-010-0018-1. [DOI:10.1007/s13273-010-0018-1]
9. Ohlsen K, Ziebuhr W, Koller K-P, Hell W, Wichelhaus TA, Hacker J. Effects of subinhibitory concentrations of antibiotics on alpha-toxin (hla) gene expression of methicillin-sensitive and methicillin-resistant Staphylococcus aureus isolates. Antimicrobial agents and chemotherapy. 1998; 42(11): 2817-23.
10. Nallanthighal S, Chan C, Bharali DJ, Mousa SA, Vásquez E, Reliene R. Particle coatings but not silver ions mediate genotoxicity of ingested silver nanoparticles in a mouse model. NanoImpact. 2017; 5: 92-100. DOI: 10.1016/j.impact.2017.01.003. [DOI:10.1016/j.impact.2017.01.003]
11. Hwang ET, Lee JH, Chae YJ, Kim YS, Kim BC, Sang BI, et al. Analysis of the Toxic Mode of Action of Silver Nanoparticles Using Stress‐Specific Bioluminescent Bacteria. Small. 2008; 4(6): 746-50. DOI:10.1002/smll.200700954. [DOI:10.1002/smll.200700954]
12. Shin S-H, Ye M-K, Kim H-S, Kang H-S. The effects of nano-silver on the proliferation and cytokine expression by peripheral blood mononuclear cells. International journal of immunopharmacology. 2007; 7(13): 1813-8. DOI: 10.1016/j.intimp.2007.08.025. [DOI:10.1016/j.intimp.2007.08.025]
13. Soto K, Garza K, Murr L. Cytotoxic effects of aggregated nanomaterials. Acta Biomaterialia. 2007; 3(3): 351-8. DOI:10.1016/j.actbio.2006.11.004. [DOI:10.1016/j.actbio.2006.11.004]
14. Wijnhoven SW, Peijnenburg WJ, Herberts CA, Hagens WI, Oomen AG, Heugens EH, et al. Nano-silver–a review of available data and knowledge gaps in human and environmental risk assessment. Nanotoxicology. 2009; 3(2): 109-38. DOI: 10.1080/17435390902725914. [DOI:10.1080/17435390902725914]
15. Amini B, Baghchesaraie H, Haji Ojagh Faghihi M. Effect of different sub MIC concentrations of penicillin, vancomycin and ceftazidime on morphology and some biochemical properties of Staphylococcus aureus and Pseudomonas aeruginosa isolates. Iranian Journal of Microbiology. 2009; 1(1): 43-7.
16. Doss S, Tillotson G, Amyes S. Effect of sub‐inhibitory concentrations of antibiotics on the virulence of Staphylococcus aureus. Journal of applied bacteriology . 1993; 75(2):123-8. [DOI:10.1111/j.1365-2672.1993.tb02756.x]
17. Gambino M, Marzano V, Villa F, Vitali A, Vannini C, Landini P, Cappitelli F. Effects of sublethal doses of silver nanoparticles on Bacillus subtilis planktonic and sessile cells. Journal of applied microbiology. 2015; 118(5): 1103-15. DOI: 10.1111/jam.12779. [DOI:10.1111/jam.12779]
18. Yang Y, Alvarez PJ. Sublethal concentrations of silver nanoparticles stimulate biofilm development. Environmental Science and Technology Letters. 2015; 2(8): 221-6. DOI: 10.1021/acs.estlett.5b00159. [DOI:10.1021/acs.estlett.5b00159]

Add your comments about this article : Your username or Email:

Send email to the article author

© 2007 All Rights Reserved | Medical Laboratory Journal