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Background & Objective: Wound infection in post-operation is one of the side effect that bothering the patients’ quality of life. This study was done to determine the effect of subdermal irrigation with normal saline on the wound infection in abdominal surgery of obstetrics and gynecology. Materials & Methods: A randomized clinical trial of subdermal irrigation with normal saline versus no irrigation was performed on 200 patients who were candidate for abdominal surgery of obstetrics and gynecology. Contaminated wound was excluded. In the case group (100 patients) after facial repair and complete hemostasis, subdermic tissue was irrigated with 1000-milliliter normal saline and skin was closed. In the control group (100 patients) after facial repair and complete hemostasis skin was closed without subdermal irrigation. All of the patients were followed until complete repair of skin. Results: 2 groups were similar in age, BMI, duration of surgery and the type of operation. Wound infection was observed in 1% of case group and in 7% of control groups, which was statistically significant (P<0.05). Conclusion: Subdermal irrigation with normal saline is effective, simple, safe and not expensive which can reduce the wound infection and can be offered particularly in high risk patients for wound infection.

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Background&Objective: The ways of prevention of wound infections are too much, and some references mentioned that one of those ways is dressing. Dressing can cause a sort of spiritual transquility for patients, too. In the other side, doing it causes some kinds of limitations such as preventing of taking a bath or spending expenses. So, we decided to consider the influence of continuation of dressing, on the rate of wound infection, after first 48 hours post operation.
Materials&Methods: In this research, we studied on 150 patients who were under the same surgeries, So that in the half of them, after 48 hours dressing were removed, and in the half remaining, dressing were changed daily for one week. In both groups, in the third, seventh and thirtieth day after surgery were inspected and examined by surgeon, in the case for having or absence of symptoms and signs of infection. In subgrouping patients we considered some factors include age, sex, type of operation and predisposing diseases. The 30 th day post operation was the end of our study and information analyzed in the computer with SPSS software.
Results: The average age in the group of without dressing was 35.12±20.19 and in the group with full dressing was 37.61±18.78 years. There were 74 men and 76 women. There was Just one wound infection in our study, who had dressing for one week. The case was woman without serious disease, and a half day before surgery was admitted in the hospital. In the group without dressing, we did not have any wound infection. 5.3% were nervous in the no dressing group and the 17.3% of patients with dressing were anxious of having their bath with delay.
Conclusion: According to the no impression of dressing on the rate of wound infection in the clean surgeries after first two days and also lack of considerable anxiety in the non dressing group, we recommend picking up the clean wounds dressing after 48 hours and make patients feel guaranteed.

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Background and Objective: Considering the significant incidence of nosocomial infections in hospitalized patients, this study was done to determine the prevalence of Staphylococcus aureus strains isolated from wound infection and drug sensitivity pattern, Tehran-Iran. Materials and Methods: In this descriptive study, Staphylococcus aureus isolated and identified according to standard procedures from the wound infections of 614 patients referred to Baqiyatallah hospital, Tehran-Iran during 2006-07. The samples were examined and antibiogram was performed by disc diffusion method on Mueller Hinton agar with 12 antibiotics. Results: 100 (16.28%) of wound infection of Staphylococcus aureus was isolated from 614 patients. The infection rate in men was twice compared to women. The highest rate 29 (29%) was observed in people aged 40 to 60 group. Also specimen's patients with immunosuppressive diseases (28 cases), surgical site infection (16 cases) and normal wounds (13 cases) were considered to be most prevalent isolates. Antibiotic sensitivity testing revealed that 96 (96%) of isolates were sensitive to vancomycin, 95 (95%) and 92 (92%) were resistant to penicillin and cotrimoxazole, respectively. Our result showed that 43% of strains were resistant at 11 antibiotics. Conclusion: This study showed that the prevalence of Staphylococcus aureus was 16.28% of samples, with 43% antibiotic resistance. The highest sensitivity was toward to vancomycin.

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Background and Objective: Wound infection treatment, particularly in chronic and bacterial poly cases, is difficult and entails heavy costs. This study was done to determine the prevalence of poly bacterial infection and antimicrobial susceptibility of wound samples from different wards. Methods: In this descriptive study, wound sampling was prepared from 336 patients admitted to different wards of Baqiatallah Hospital in Tehran, Iran. Identification was performed based on biochemical tests including oxidase test, TSI, IMVIC, lysine decarboxylase, phenylalanine deaminase, urea, motility, catalase, coagulase, mannitol fermentation, optochin sensitivity, susceptibility to bacitracin and sulfamethoxazole, growth in Bile esculin and DNase production. Antibiotic resistance pattern of isolates was determined using disk diffusion method for 14 important antibiotics. Results: 294 samples were positive for bacterial culture, from which 364 isolates including 11 different isolates were obtained. Out of 294 positive samples, 245 samples were mono bacterial and 54 were poly bacterial including two-bacterial (45 samples), three-bacterial (7 samples), and four-bacteral (2 samples). S. aureus (29.7%), Enterococci (15.6%), and E. coli (15.6%) were the most prevalent isolates. S. aureus-Enterococci pattern was the most common two-bacterial pattern (33%), and majority of polybacterial patterns belonging to gram negative bacteria was in surgery ward (32.5%). Antibiogram results showed high levels of antibiotic resistance in the isolates. Imipenem and amikacin were the most effective antibiotics against Gram negative isolates, and vancomycin for Gram positive isolates. Also, 71% of S. aureus isolates were resistant to oxacillin. Conclusion: Variation of bacterial isolates was similar to other studies. Most of poly-bacterial wound infections were due to common nosocomial pathogens and their high rates of antibiotic resistance are extremely alarming.